NCT01644565

Brief Summary

The purpose of the study is to determine if immunization with a chimeric E. coli protein, dsc14CfaE-sCT2/LTB5, is safe and immunogenic when administered by vaccination under the skin.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
57

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started Aug 2012

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 11, 2012

Completed
8 days until next milestone

First Posted

Study publicly available on registry

July 19, 2012

Completed
13 days until next milestone

Study Start

First participant enrolled

August 1, 2012

Completed
1.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2014

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2015

Completed
4.1 years until next milestone

Results Posted

Study results publicly available

July 8, 2019

Completed
Last Updated

February 12, 2021

Status Verified

February 1, 2021

Enrollment Period

1.8 years

First QC Date

July 11, 2012

Results QC Date

November 30, 2016

Last Update Submit

February 10, 2021

Conditions

Outcome Measures

Primary Outcomes (1)

  • Safety - Occurrence of Adverse Events

    Occurrence of related and unrelated to vaccine AE's

    1 year

Secondary Outcomes (2)

  • Number of Participants With Immune Responses to Vaccine Antigens

    baseline and post dose

  • Antigen-Specific IgA Geometric Mean Titers

    Day 0, 21,42, 56, 70

Study Arms (9)

Group A-1

EXPERIMENTAL

Recombinant fimbrial adhesin dscCfaE: 1 ug of dscCfaE ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dscCfaE

Group A-2

EXPERIMENTAL

Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5: 2.6 ug of Chimera ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5

Group A-3

EXPERIMENTAL

Modified E. coli heat labile enterotoxin LTR192G: 100 ng of LTR192G ID on study days 0, 21 and 42

Biological: Modified E. coli heat labile enterotoxin LTR192G

Group B-1

EXPERIMENTAL

Recombinant fimbrial adhesin dscCfaE and Modified E. coli heat labile enterotoxin LTR192G: 1 ug of dscCfaE + 100 ng of LTR192G ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dscCfaEBiological: Modified E. coli heat labile enterotoxin LTR192G

Group B-2

EXPERIMENTAL

Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5 and Modified E. coli heat labile enterotoxin LTR192G: 2.6 ug of Chimera + 100 ng of LTR192G ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5Biological: Modified E. coli heat labile enterotoxin LTR192G

Group C-1

EXPERIMENTAL

Recombinant fimbrial adhesin dscCfaE and Modified E. coli heat labile enterotoxin LTR192G: 5 ug of dscCfaE + 100 ng of LTR192G ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dscCfaEBiological: Modified E. coli heat labile enterotoxin LTR192G

Group C-2

EXPERIMENTAL

Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5 and Modified E. coli heat labile enterotoxin LTR192G: 12.9 ug of Chimera + 100 ng of LTR192G ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dsc14CfaE-sCTA2/LTB5Biological: Modified E. coli heat labile enterotoxin LTR192G

Group D-1

EXPERIMENTAL

Recombinant fimbrial adhesin dscCfaE and Modified E. coli heat labile enterotoxin LTR192G: 25 ug dscCfaE + 100 ng LTR192G ID on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dscCfaEBiological: Modified E. coli heat labile enterotoxin LTR192G

Group D-2

EXPERIMENTAL

Recombinant fimbrial adhesin dscCfaE and Modified E. coli heat labile enterotoxin LTR192G: 1250 ug dscCfaE + 50 ng LTR192G TCI on study days 0, 21 and 42

Biological: Recombinant fimbrial adhesin dscCfaEBiological: Modified E. coli heat labile enterotoxin LTR192G

Interventions

Also known as: Chimera
Group A-2Group B-2Group C-2
Also known as: dscCfaE
Group A-1Group B-1Group C-1Group D-1Group D-2
Also known as: LTR192G
Group A-3Group B-1Group B-2Group C-1Group C-2Group D-1Group D-2

Eligibility Criteria

Age18 Years - 45 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy, adult, male or female, age 18 to 45 years (inclusive) at the time of enrollment.
  • Completion and review of comprehension test (achieved \> 70% accuracy).
  • Signed informed consent document.
  • Available for the required follow-up period and scheduled clinic visits.
  • Women: Negative pregnancy test with understanding (through informed consent process) to not become pregnant during the study or within three (3) months following study completion.

You may not qualify if:

  • Health problems (for example, chronic medical conditions such as psychiatric conditions, diabetes mellitus, hypertension or any other conditions that might place the subjects at increased risk of adverse events. Study clinicians, in consultation with the PI, will use clinical judgment on a case-by-case basis to assess safety risks under this criterion. The PI will consult with the Research Monitor as appropriate.
  • Clinically significant abnormalities on physical examination.
  • Use of immunosuppressive medications (systemic corticosteroids or chemotherapeutics that may influence antibody development), or immunosuppressive illness, including IgA deficiency (defined by serum IgA below the detectable limit).
  • Women who are pregnant or planning to become pregnant during the study period plus 3 months beyond the last study safety visit and currently nursing women.
  • Participation in research involving another investigational product (defined as receipt of investigational product or exposure to invasive investigational device) 30 days before planned date of first vaccination or anytime through the last study safety visit.
  • Positive blood test for HBsAg, HCV, HIV-1.
  • Clinically significant abnormalities on basic laboratory screening.
  • History of chronic skin disease (clinician judgment).
  • History of atopy such as active eczema.
  • Acute skin infection/eruptions on the upper arms including fungal infections, severe acne or active contact dermatitis.
  • Allergies that may increase the risk of AEs.
  • Regular use (weekly or more often) of antidiarrheal, anti-constipation, or antacid therapy.
  • Abnormal stool pattern (fewer than 3 stools per week or more than 3 stools per day) on a regular basis; loose or liquid stools on other than an occasional basis.
  • Prior exposure to ETEC or Vibrio cholera.
  • History of microbiologically confirmed ETEC or cholera infection.
  • +3 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Walter Reed Army Institute of Research Clinical trial Center

Silver Spring, Maryland, 20910, United States

Location

MeSH Terms

Conditions

Escherichia coli Infections

Condition Hierarchy (Ancestors)

Enterobacteriaceae InfectionsGram-Negative Bacterial InfectionsBacterial InfectionsBacterial Infections and MycosesInfections

Results Point of Contact

Title
Ramiro L. Gutierrez, MD, MPH
Organization
Enteric Diseases Department, Naval Medical Research Center

Study Officials

  • Ramiro L. Gutierrez, MD, MPH

    Enteric Diseases Department, Naval Medical Research Center

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
FED
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 11, 2012

First Posted

July 19, 2012

Study Start

August 1, 2012

Primary Completion

June 1, 2014

Study Completion

June 1, 2015

Last Updated

February 12, 2021

Results First Posted

July 8, 2019

Record last verified: 2021-02

Locations