NCT03150121

Brief Summary

Screening programs for high-grade ovarian carcinoma failed to reduce disease-specific mortality, since they do not offer early enough detection of the disease. Most cases of high grade ovarian cancer develop in the fallopian tubes, hence the universal recommendation for high-risk populations (e.g., BRCA1/2 mutation carriers) is to undergo risk-reducing bilateral salpingo-oophorectomy (RRBSO) around the age of 40. The aims of this trial are: (1) to identify novel early-stage disease biomarkers using liquid biopsies obtained through uterine lavage, and (2) to optimize the technique a of uterine lavage and the processing of the samples for ultimate implementation as a routine diagnostic test for high risk populations.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
500

participants targeted

Target at P75+ for not_applicable ovarian-cancer

Timeline
Completed

Started Jun 2014

Longer than P75 for not_applicable ovarian-cancer

Geographic Reach
1 country

4 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 29, 2014

Completed
2.8 years until next milestone

First Submitted

Initial submission to the registry

May 3, 2017

Completed
9 days until next milestone

First Posted

Study publicly available on registry

May 12, 2017

Completed
5.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2022

Completed
2 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2024

Completed
Last Updated

February 26, 2020

Status Verified

February 1, 2020

Enrollment Period

8.5 years

First QC Date

May 3, 2017

Last Update Submit

February 24, 2020

Conditions

Outcome Measures

Primary Outcomes (1)

  • Ability of uterine lavage-based biomarkers to detect early stage ovarian cancer

    Sensitivity and specificity of proteomic and genomic biomarkers measured in uterine lavage liquid biopsies. The samples will be analyzed in a blinded fashion. The pathological data of the patients will be reviewed and the sensitivity/specificity of each biomarker will be calculated

    7 years

Secondary Outcomes (2)

  • Complications rate of uterine lavage procedure in high risk population

    7 years

  • Burden of uterine lavage procedure as routine diagnostic test for high risk population

    7 years

Study Arms (3)

Ovarian cancer patients

EXPERIMENTAL

High grade ovarian/fallopian tube/primary peritoneal carcinoma patients with current active disease, at any stage and histological type, who have not yet undergone debulking surgery.

Procedure: Uterine lavageProcedure: Blood sampleDevice: Uterine lavage catheter

Non-malignant controls

ACTIVE COMPARATOR

Patients with non-malignant gynecological conditions indicating surgical procedure, either salpingo-oophorectomy, hysterectomy or hysteroscopy.

Procedure: Uterine lavageProcedure: Blood sampleDevice: Uterine lavage catheter

High risk population

EXPERIMENTAL

Healthy women with genetically high risk for developing ovarian cancer, who have not undergone risk reducing procedure.

Procedure: Uterine lavageProcedure: Blood sampleDevice: Uterine lavage catheter

Interventions

Uterine lavage is performed using an intrauterine insemination catheter or rigid pipelle uterine sampler. The catheter is inserted into the cervical canal. 10mL of saline are infused into the uterine cavity and fallopian tubes and immediately retrieved.

Also known as: Tubo-uterine lavage
High risk populationNon-malignant controlsOvarian cancer patients
Blood samplePROCEDURE

5-10mL of blood will be drawn from participants.

High risk populationNon-malignant controlsOvarian cancer patients

Uterine lavage is performed using an intrauterine insemination catheter or rigid pipelle uterine sampler. The catheter is inserted into the cervical canal. 10mL of saline are infused into the uterine cavity and fallopian tubes and immediately retrieved.

Also known as: Intrauterine insemination catheter
High risk populationNon-malignant controlsOvarian cancer patients

Eligibility Criteria

Age18 Years - 80 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsRelevant only for female participants
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Subject must be at least 18 years of age.
  • Subject must have all gynecological organs, including: vagina, cervix, uterus, fallopian tubes and ovaries.
  • For the proof-of-principle cohort:
  • Subject must have been evaluated for a gynecologic condition, for which the treating physician has determined that a surgical intervention is required. The surgical procedure may be either: hysterectomy, surgical hysteroscopy, or salpingo-oophorectomy.
  • The eligible gynecological diagnoses include: high grade ovarian, fallopian tube or primary peritoneal carcinoma, or any non-malignant gynecologic condition.
  • For the high risk cohort:
  • Subject must have an increased risk for developing ovarian cancer, as determined by genetic testing for BRCA1 or BRCA2 mutations, or by family history of at least one first degree relative that has been diagnosed with high grade ovarian cancer.

You may not qualify if:

  • Subject is pregnant or is currently attempting to conceive.
  • Subject has undergone resection of the uterus, fallopian tubes or ovaries.
  • Subject is unable to read, understand and sign the informed consent form.
  • Subject refuses to allow access to medical records or pathology reports.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (4)

Shaare Zedek Medical Center

Jerusalem, 91031, Israel

NOT YET RECRUITING

Meir Medical Center

Kfar Saba, 44281, Israel

RECRUITING

Rabin Medical Center

Petah Tikva, 49414, Israel

RECRUITING

Sheba Medical Center

Ramat Gan, 52621, Israel

RECRUITING

Related Publications (1)

  • Bahar-Shany K, Barnabas GD, Deutsch L, Deutsch N, Glick-Saar E, Dominissini D, Sapoznik S, Helpman L, Perri T, Blecher A, Katz G, Yagel I, Rosenblatt O, Shai D, Brandt B, Meyer R, Mohr-Sasson A, Volodarsky-Perel A, Zilberman I, Armon S, Jakobson-Setton A, Eitan R, Kadan Y, Beiner M, Josephy D, Brodsky M, Friedman E, Anafi L, Molchanov Y, Korach J, Geiger T, Levanon K. Proteomic signature for detection of high-grade ovarian cancer in germline BRCA mutation carriers. Int J Cancer. 2023 Feb 15;152(4):781-793. doi: 10.1002/ijc.34318. Epub 2022 Oct 23.

MeSH Terms

Conditions

Ovarian NeoplasmsFallopian Tube NeoplasmsCystadenocarcinoma, Serous

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Endocrine Gland NeoplasmsNeoplasms by SiteNeoplasmsOvarian DiseasesAdnexal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital Neoplasms, FemaleUrogenital NeoplasmsGenital DiseasesEndocrine System DiseasesGonadal DisordersFallopian Tube DiseasesCystadenocarcinomaAdenocarcinomaCarcinomaNeoplasms, Glandular and EpithelialNeoplasms by Histologic TypeNeoplasms, Cystic, Mucinous, and Serous

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • Keren Levanon, MD,PhD

    Sheba Medical Center

    PRINCIPAL INVESTIGATOR
  • Yfat Kadan, MD

    Meir Medical Center

    PRINCIPAL INVESTIGATOR
  • Ram Eitan, MD

    Rabin Medical Center

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
PARALLEL
Sponsor Type
OTHER GOV
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 3, 2017

First Posted

May 12, 2017

Study Start

June 29, 2014

Primary Completion

December 31, 2022

Study Completion

December 31, 2024

Last Updated

February 26, 2020

Record last verified: 2020-02

Data Sharing

IPD Sharing
Will not share

Locations