Subgingival Microbiota Following Mechanical Treatment and it's Effect on Peri-implant Sub-mucosa in Periodontitis Patients

NCT03090282 | Completed

• 1 other identifier: PKUSSIRB-201525102
• Study Type: observational
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The investigators planed to observe the microbiome of an aggressive periodontitis patient, during mechanical treatment, surgery(if indicated) and implant placement. The investigators designed to observe the changes after mechanical treatment.Then, the investigators will compare the sub-mucosa microbiome of implant and the subgingival community. The investigators expected a different community after treatment, and there could be similarity between implant and nature teeth.

Conditions

Aggressive Periodontitis, Generalized

Keywords

mechanical treatment; implant

Outcome Measures

Primary Outcomes (4)

• changes of diversity of subgingival community (compare the sub-mucosa microbiome of implant and the subgingival community)

  the diversity of subgingival community before and after mechanical treatment

  Baseline, 6 weeks after mechanical treatment

• changes of diversity of subgingival community (compare the sub-mucosa microbiome of implant and the subgingival community)

  the diversity of submucosa community before and after implatation

  before implantation, 1 months after second stage surgery, and every month after prosthetic treatment until 2 years.

• changes of prevalence and abundance of OTUs

  compute and compare the prevalence and abundance of OTUs

  Baseline, 6 weeks after mechanical treatment

• changes of prevalence and abundance of OTUs

  compute and compare the prevalence and abundance of OTUs

  before implantation, 1 months after second stage surgery, and every month after prosthetic treatment until 2 years.

Secondary Outcomes (3)

• changes of bleeding index

  Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years.

• changes of probing depth

  Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years.

• clinical attachment loss

  Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years.

Interventions

mechanical treatment(mainly scaling and root planing), periodontal surgery(if indicated), implant placement. PROCEDURE

The investigators shall follow the regular procedure to these patients.

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

aggressive periodontitis patients with defection of dentition or shall extracted hopeless teeth, and intended to implant treatment.

You may qualify if:

• Subjects were between 18-35 years old and diagnosed with generalized aggressive periodontitis (GAgP) according to the 1999 International Classification of Periodontal Diseases (Armitage 1999);
• At least 20 teeth in the first appointment and at least 3 teeth(other than first molars and central incisors)with a probing depth(PD) ≥ 6mm, clinical attachment level (CAL)≥5mm;
• The clinical diagnosis was confirmed by evidence of inter-proximal bone loss on full-mouth periapical radiographs;
• Family history of aggressive periodontitis.

You may not qualify if:

• Uncontrolled systemic conditions including diabetes and hypertension;
• Pregnant or lactating;
• Smoking;
• Received periodontal treatment within the previous 6 months or antibiotic medication during the previous 3 months.

Sponsors & Collaborators

• Peking University: lead

Study Sites (1)

Peking university hospital of stomatology

Beijing, Beijing Municipality, 100081, China

Location

Related Publications (1)

• Liu G, Luan Q, Chen F, Chen Z, Zhang Q, Yu X. Shift in the subgingival microbiome following scaling and root planing in generalized aggressive periodontitis. J Clin Periodontol. 2018 Apr;45(4):440-452. doi: 10.1111/jcpe.12862. Epub 2018 Mar 6.

  PMID: 29266363 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

some bacteria DNA from subgingival. No need for human DNA.

MeSH Terms

Conditions

Aggressive Periodontitis

Interventions

Root Planing

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Intervention Hierarchy (Ancestors)

Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Officials

• Yu xiaoqian

  Peking University Hospital of Stomatology

  STUDY CHAIR

• Liu guojing

  Peking University Hospital of Stomatology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: March 9, 2017
• First Posted: March 24, 2017
• Study Start: April 1, 2016
• Primary Completion: December 1, 2020
• Study Completion: March 1, 2022
• Last Updated: March 29, 2023

Record last verified: 2023-03

Locations

CN (1)