GCF HIF-1α, VEGF and TNF-α Levels in G-AgP Patients Before and After Periodontal Treatment.

NCT04020315 | Completed

• 1 other identifier: AggressiveHIF
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Hypoxia-inducible angiogenic pathway involving hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF) and tumour necrosis factor- alpha (TNF-α) may regulate the several biological processes related to inflammation. Generalized aggressive periodontitis (G-AgP) is a rare but highly destructive form of inflammatory periodontal disease. The present study aimed to assess the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) HIF-1α, VEGF and TNF-α levels in G-AgP patients. 20 G-AgP and 20 periodontally healthy subjects were enrolled. At baseline, GCF samples were collected and whole mouth clinical periodontal parameters were recorded. G-AgP patients received non-surgical periodontal treatment. Clinical parameters and GCF cytokines were re-measured at 1 and 3 months after treatment. GCF HIF-1α, VEGF and TNF-α levels were analyzed by ELISA. Data were analyzed using appropriate statistical tests.

Conditions

Aggressive Periodontitis, Generalized; Periodontal Health

Keywords

aggressive periodontitis; gingival crevicular fluid; hypoxia; cytokines

Outcome Measures

Primary Outcomes (1)

• Gingival crevicular fluid protein content change

  pg/ml

  Change from baseline to 1 month and 3 months after treatment

Study Arms (1)

Generalized aggressive periodontitis

EXPERIMENTAL

Non-surgically performed scaling and root planing.

Procedure: Non-surgically performed scaling and root planing

Interventions

Non-surgically performed scaling and root planing PROCEDURE

Non-surgically performed scaling and root planing

Generalized aggressive periodontitis

Eligibility Criteria

• Age: 25 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• aged 25 to 45 years
• non-smokers with no history of smoking
• having at least 20 natural teeth.

You may not qualify if:

• having any diagnosed medical disorders such as diabetes mellitus, cardiovascular diseases, rheumatoid arthritis, immunological and mucocutaneous diseases
• usage of antibiotics, non-steroidal anti-inflammatory drugs and immunosuppressive agents within the past 6 months
• having any non-inflammatory destructive periodontal disease
• nonsurgical/surgical periodontal therapy received in the past year
• having a restorative and endodontic treatment requirement
• having orthodontic appliances or a removable partial denture
• pregnant/ lactating/ postmenopausal females.

Sponsors & Collaborators

• Aydin Adnan Menderes University: lead

Study Sites (1)

Adnan Menderes University, Faculty of Dentistry, Department of Periodontology

Aydin, 09100, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Aggressive Periodontitis; Hypoxia

Interventions

Root Planing

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: July 12, 2019
• First Posted: July 16, 2019
• Study Start: December 1, 2017
• Primary Completion: August 30, 2018
• Study Completion: September 15, 2018
• Last Updated: July 17, 2019

Record last verified: 2019-07

Locations

TU (1)