NCT02997969

Brief Summary

The purpose of this study is to evaluate the safety and immune response to an HIV clade C DNA vaccine and to an MF59-adjuvanted clade C Env protein in healthy, HIV-uninfected adults.

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
132

participants targeted

Target at P75+ for phase_1 hiv-infections

Timeline
Completed

Started Jun 2016

Typical duration for phase_1 hiv-infections

Geographic Reach
2 countries

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 21, 2016

Completed
6 months until next milestone

First Submitted

Initial submission to the registry

December 16, 2016

Completed
4 days until next milestone

First Posted

Study publicly available on registry

December 20, 2016

Completed
1.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 11, 2018

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

July 11, 2018

Completed
Last Updated

June 21, 2019

Status Verified

June 1, 2019

Enrollment Period

2.1 years

First QC Date

December 16, 2016

Last Update Submit

June 19, 2019

Conditions

Keywords

HIV

Outcome Measures

Primary Outcomes (10)

  • Frequency of severe local and systemic reactogenicity signs and symptoms (pain, tenderness, erythema, induration, fever, malaise/fatigue, myalgia, headache, nausea, vomiting, chills, arthralgia)

    Measured through Month 12

  • Frequency of adverse events (AEs)

    By body system, Medical Dictionary for Regulatory Activities (MedDRA) preferred term, severity, and assessed relationship to study products

    Measured through Month 12

  • Frequency of serious adverse events (SAEs), adverse events of special interest (AESIs), and new chronic conditions (requiring medical intervention for 30 days or more)

    Measured through Month 12

  • Composite of safety laboratory measures: white blood cells (WBC), neutrophils, lymphocytes, hemoglobin, platelets, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), and creatinine

    Measured through Month 12

  • Frequency of AEs leading to early participant withdrawal or early discontinuation of study products administration

    Measured through Month 12

  • HIV-specific total immunoglobulin G (IgG) binding antibody response breadth and magnitude as assessed by multiplex assay

    Measured through Month 6.5

  • Anti-V1/V2 scaffold IgG binding antibody responses as assessed by multiplex assay

    Measured through Month 6.5

  • Presence of neutralizing antibody responses against HIV-1 isolates

    Measured through Month 6.5

  • HIV-specific CD4+ T-cell responses as assessed by flow cytometry

    Measured through Month 6.5

  • HIV-specific CD8+ T-cell responses as assessed by flow cytometry

    Measured through Month 6.5

Study Arms (6)

Group 1: DNA + Placebo + Protein/MF59

ACTIVE COMPARATOR

Participants will receive the DNA-HIV-PT123 vaccine in the left deltoid at months 0, 1, 3, and 6. They will receive placebo in the right deltoid at months 0 and 1, and the Protein/MF59 vaccine at months 3 and 6. All injections are via needle and syringe.

Biological: DNA-HIV-PT123 vaccineBiological: Protein/MF59 vaccineBiological: Placebo

Group 2: DNA + Placebo + Protein/MF59

ACTIVE COMPARATOR

Participants will receive the DNA-HIV-PT123 vaccine in the left deltoid at months 0, 1, and 6. They will receive placebo in both deltoids at month 3, and the Protein/MF59 vaccine at months 0, 1, and 6. All injections are via needle and syringe.

Biological: DNA-HIV-PT123 vaccineBiological: Protein/MF59 vaccineBiological: Placebo

Group 3: Placebo

PLACEBO COMPARATOR

Participants will receive placebo in both deltoids at months 0, 1, 3, and 6. All injections are via needle and syringe.

Biological: Placebo

Group 4: DNA + Placebo + Protein/MF59

ACTIVE COMPARATOR

Participants will receive the DNA-HIV-PT123 vaccine via Biojector in the left deltoid at months 0, 1, 3, and 6. They will receive placebo in the right deltoid at months 0 and 1, and the Protein/MF59 vaccine at months 3 and 6, via needle and syringe.

Biological: DNA-HIV-PT123 vaccineBiological: Protein/MF59 vaccineBiological: Placebo

Group 5: DNA + Placebo + Protein/MF59

ACTIVE COMPARATOR

Participants will receive the DNA-HIV-PT123 vaccine at months 0, 1, and 6, and placebo at month 3, in the left deltoid via Biojector. They will receive placebo at month 3, and the Protein/MF59 vaccine at months 0, 1, and 6, in the right deltoid via needle and syringe.

Biological: DNA-HIV-PT123 vaccineBiological: Protein/MF59 vaccineBiological: Placebo

Group 6: Placebo

PLACEBO COMPARATOR

Participants will receive placebo in the left deltoid via Biojector, and in the right deltoid via needle and syringe, at months 0, 1, 3, and 6.

Biological: Placebo

Interventions

Contains a mixture of 3 DNA plasmids in a 1:1:1 ratio, each at 1.33 mg: 1) clade C ZM96 gag, 2) clade C ZM96 gp140, and 3) clade C CN54 pol-nef, delivered at a total dose 4 mg administered as 1 mL intramuscularly (IM)

Group 1: DNA + Placebo + Protein/MF59Group 2: DNA + Placebo + Protein/MF59Group 4: DNA + Placebo + Protein/MF59Group 5: DNA + Placebo + Protein/MF59

clade C TV1.C gp120 Env and clade C 1086.C gp120 Env, each at a dose of 100 mcg, mixed with MF59 adjuvant, administered as 0.5 mL IM

Also known as: Bivalent Subtype C gp120/MF59
Group 1: DNA + Placebo + Protein/MF59Group 2: DNA + Placebo + Protein/MF59Group 4: DNA + Placebo + Protein/MF59Group 5: DNA + Placebo + Protein/MF59
PlaceboBIOLOGICAL

Sodium Chloride, 0.9%, administered by IM injection at volumes to match the active products

Also known as: Sodium Chloride, 0.9%
Group 1: DNA + Placebo + Protein/MF59Group 2: DNA + Placebo + Protein/MF59Group 3: PlaceboGroup 4: DNA + Placebo + Protein/MF59Group 5: DNA + Placebo + Protein/MF59Group 6: Placebo

Eligibility Criteria

Age18 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • General and Demographic Criteria
  • Age of 18 to 40 years
  • Access to a participating HVTN CRS and willingness to be followed for the planned duration of the study
  • Ability and willingness to provide informed consent
  • Assessment of understanding: volunteer demonstrates understanding of this study; provides answers to a questionnaire prior to first vaccination with verbal demonstration of understanding of all questionnaire items answered incorrectly
  • Agrees not to enroll in another study of an investigational research agent
  • Good general health as shown by medical history, physical exam, and screening laboratory tests
  • HIV-Related Criteria:
  • Willingness to receive HIV test results
  • Willingness to discuss HIV infection risks and amenable to HIV risk reduction counseling.
  • Assessed by the clinic staff as being at "low risk" for HIV infection and committed to maintaining behavior consistent with low risk of HIV exposure through the last required protocol clinic visit.
  • Hemogram/Complete blood count (CBC)
  • Hemoglobin ≥ 11.0 g/dL for volunteers who were born female, ≥ 13.0 g/dL for volunteers who were born male
  • White blood cell count = 3,300 to 12,000 cells/mm\^3
  • Total lymphocyte count ≥ 800 cells/mm\^3
  • +25 more criteria

You may not qualify if:

  • General
  • Blood products received within 120 days before first vaccination
  • Investigational research agents received within 30 days before first vaccination
  • Body mass index (BMI) ≥ 40; or BMI ≥ 35 with 2 or more of the following: systolic blood pressure \> 140 mm Hg, diastolic blood pressure \> 90 mm Hg, current smoker, known hyperlipidemia
  • Intent to participate in another study of an investigational research agent during the planned duration of the HVTN 111 study
  • Pregnant or breastfeeding Vaccines and other Injections
  • HIV vaccine(s) received in a prior HIV vaccine trial. For volunteers who have received control/placebo in an HIV vaccine trial, the HVTN 111 PSRT will determine eligibility on a case-by-case basis.
  • Non-HIV experimental vaccine(s) received within the last 5 years in a prior vaccine trial. Exceptions may be made for vaccines that have subsequently undergone licensure by the FDA. For volunteers who have received control/placebo in an experimental vaccine trial, the HVTN 111 PSRT will determine eligibility on a case-by-case basis. For volunteers who have received an experimental vaccine(s) greater than 5 years ago, eligibility for enrollment will be determined by the HVTN 111 PSRT on a case-by-case basis.
  • Live attenuated vaccines other than influenza vaccine received within 30 days before first vaccination or scheduled within 14 days after injection (eg, measles, mumps, and rubella \[MMR\]; oral polio vaccine \[OPV\]; varicella; yellow fever)
  • Influenza vaccine or any vaccines that are not live attenuated vaccines and were received within 14 days prior to first vaccination (eg, tetanus, pneumococcal, Hepatitis A or B)
  • Allergy treatment with antigen injections within 30 days before first vaccination or that are scheduled within 14 days after first vaccination Immune System
  • Immunosuppressive medications received within 168 days before first vaccination. (Not excluded from participation: \[1\] corticosteroid nasal spray; \[2\] inhaled corticosteroids; \[3\] topical corticosteroids for mild, uncomplicated dermatitis; or \[4\] a single course of oral/parenteral corticosteroids at doses \< 2 mg/kg/day and length of therapy \< 11 days with completion at least 30 days prior to enrollment.
  • Serious adverse reactions to vaccines or to vaccine components such as eggs, egg products, or neomycin, including history of anaphylaxis and related symptoms such as hives, respiratory difficulty, angioedema, and/or abdominal pain. (Not excluded: a volunteer who had a nonanaphylactic adverse reaction to pertussis vaccine as a child.)
  • Immunoglobulin received within 60 days before first vaccination
  • Autoimmune disease
  • +27 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (5)

Aurum Tembisa CRS

Johannesburg, Gauteng, 1632, South Africa

Location

Isipingo CRS

Westville, KwaZulu-Natal, 3630, South Africa

Location

Aurum Institute Klerksdorp CRS

Klerksdorp, North West, 2571, South Africa

Location

National Institute for Medical Research (NIMR) - Mbeya Medical Research Center (MMRC) Network CRS

Mbeya, Tanzania

Location

Matero Reference Clinic CRS

Lusaka, Zambia

Location

Related Publications (3)

  • Moodie Z, Li SS, Giorgi EE, Williams LD, Dintwe O, Carpp LN, Chen S, Seaton KE, Sawant SS, Zhang L, Heptinstall J, Liu S, Grunenberg N, Tomaka F, Rerks-Ngarm S, Pitisuttithum P, Nitayaphan S, Ake JA, Vasan S, Pantaleo G, Frank I, Baden LR, Goepfert PA, Keefer M, Chirenje M, Hosseinipour MC, Mngadi K, Laher F, Garrett N, Bekker LG, De Rosa S, Andersen-Nissen E, Kublin JG, Lu S, Gilbert PB, Gray GE, Corey L, McElrath MJ, Tomaras GD. A polyvalent DNA prime with matched polyvalent protein/GLA-SE boost regimen elicited the most robust and broad IgG and IgG3 V1V2 binding antibody and CD4+ T cell responses among 13 HIV vaccine trials. Emerg Microbes Infect. 2025 Dec;14(1):2485317. doi: 10.1080/22221751.2025.2485317. Epub 2025 Apr 7.

  • Hanass-Hancock J, Carpenter B, Reddy T, Nzuza A, Gaffoor Z, Goga A, Andrasik M. Participants' characteristics and motivations to screen for HIV vaccine and monoclonal antibody trials in KwaZulu-Natal, South Africa. Trials. 2021 Dec 11;22(1):897. doi: 10.1186/s13063-021-05792-7.

  • Hosseinipour MC, Innes C, Naidoo S, Mann P, Hutter J, Ramjee G, Sebe M, Maganga L, Herce ME, deCamp AC, Marshall K, Dintwe O, Andersen-Nissen E, Tomaras GD, Mkhize N, Morris L, Jensen R, Miner MD, Pantaleo G, Ding S, Van Der Meeren O, Barnett SW, McElrath MJ, Corey L, Kublin JG; HVTN 111 Protocol Team. Phase 1 Human Immunodeficiency Virus (HIV) Vaccine Trial to Evaluate the Safety and Immunogenicity of HIV Subtype C DNA and MF59-Adjuvanted Subtype C Envelope Protein. Clin Infect Dis. 2021 Jan 23;72(1):50-60. doi: 10.1093/cid/ciz1239.

MeSH Terms

Conditions

HIV Infections

Interventions

Sodium Chloride

Condition Hierarchy (Ancestors)

Blood-Borne InfectionsCommunicable DiseasesInfectionsSexually Transmitted Diseases, ViralSexually Transmitted DiseasesLentivirus InfectionsRetroviridae InfectionsRNA Virus InfectionsVirus DiseasesGenital DiseasesUrogenital DiseasesImmunologic Deficiency SyndromesImmune System Diseases

Intervention Hierarchy (Ancestors)

ChloridesHydrochloric AcidChlorine CompoundsInorganic ChemicalsSodium Compounds

Study Officials

  • Mina Hosseinipour

    UNC Project- Lilongwe

    STUDY CHAIR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 16, 2016

First Posted

December 20, 2016

Study Start

June 21, 2016

Primary Completion

July 11, 2018

Study Completion

July 11, 2018

Last Updated

June 21, 2019

Record last verified: 2019-06

Data Sharing

IPD Sharing
Will not share

Locations