NCT02959762

Brief Summary

Animal studies have found that vitamin K-dependent proteins matrix Gla protein and osteocalcin beneficially influence lipid and glucose metabolism, respectively. However, this concept has not been tested in humans at risk for dyslipidemia and diabetes risk. Vitamin K supplementation presents an opportunity to test the hypothesized link between the vitamin K-dependent proteins and markers of lipid and glucose metabolism. The investigators will conduct an 8-week vitamin K intervention (to manipulate carboxylation of matrix Gla protein and osteocalcin) and determine its effects on markers of dyslipidemia and diabetes risk. Sixty obese children will be randomly allocated to either the control group receiving placebo or the low-dose (45 mcg/d) or high-dose group (90 mcg/d) receiving vitamin K (menaquinone-7).

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
30

participants targeted

Target at below P25 for not_applicable obesity

Timeline
Completed

Started Oct 2016

Longer than P75 for not_applicable obesity

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 1, 2016

Completed
10 days until next milestone

First Submitted

Initial submission to the registry

October 11, 2016

Completed
29 days until next milestone

First Posted

Study publicly available on registry

November 9, 2016

Completed
4.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2020

Completed
29 days until next milestone

Study Completion

Last participant's last visit for all outcomes

December 30, 2020

Completed
Last Updated

November 20, 2019

Status Verified

November 1, 2019

Enrollment Period

4.2 years

First QC Date

October 11, 2016

Last Update Submit

November 18, 2019

Conditions

Keywords

G01 [Biological Sciences]G02.513 [Nutrition]G06.696.259 [Child Nutrition]G07.553.481.398.571 [Obesity]G06 [Biochemical Phenomena, Metabolism, and Nutrition]G12.392.617 [Insulin Resistance]G06.696.259.500 [Adolescent Nutrition]A06 [Endocrine System]A07 [Cardiovascular System]C18.452.297.681 [Obesity in Diabetes]C18.452.555 [Insulin Resistance]C18 [Nutritional and Metabolic Diseases]C18.452.494 [Hyperlipidemia]C18.452.460 [Hyperglycemia]C14 [Cardiovascular Diseases]D11.786.875.844 [Vitamin K 2]D02.806.550.750 [Vitamin K 2]E02.293 [Diet Therapy]E02 [Therapeutics]F04.096.544.215.508.428 [Primary Prevention]N01.224.425.525 [Nutritional Status]

Outcome Measures

Primary Outcomes (3)

  • Change in serum lipid concentrations

    To determine if the vitamin K-induced change in matrix Gla protein carboxylation improves fasting lipid panel (triglycerides, total cholesterol, HDL-cholesterol, and LDL-cholesterol) in a dose-dependent manner.

    8 weeks

  • Change in insulin sensitivity

    To determine if the vitamin K-induced change in osteocalcin carboxylation effects insulin sensitivity in a dose-dependent manner. Insulin sensitivity will be calculated from plasma insulin and glucose concentrations measured during a two-hour oral glucose tolerance test by using the oral glucose minimal model.

    8 weeks

  • Change in beta-cell function

    To determine if the vitamin K-induced change in osteocalcin carboxylation effects beta-cell function in a dose-dependent manner. Beta-cell function, as assessed by dynamic beta-cell responsitivity, will be calculated from plasma glucose and C-peptide concentrations measured during a two-hour oral glucose tolerance test by using the oral C-peptide minimal model.

    8-weeks

Secondary Outcomes (3)

  • Change in coagulation

    8 weeks

  • Change in arterial stiffness (pulse wave velocity)

    8 weeks

  • Change in endothelial function (flow-mediated dilation)

    8 weeks

Study Arms (3)

Placebo-Control

PLACEBO COMPARATOR

The placebo-control group will take two placebo softgel capsules every day for 8 weeks.

Dietary Supplement: Placebo-Control

Low-Dose Vitamin K2 (45-mcg/d)

ACTIVE COMPARATOR

The low-dose vitamin K2 group will take one 45-mcg vitamin K2 softgel capsule and one placebo softgel capsule every day for 8 weeks.

Dietary Supplement: Low-Dose Vitamin K2 (menaquinone-7; 45-mcg/d)

High-Dose Vitamin K2 (90-mcg/d)

ACTIVE COMPARATOR

The high-dose vitamin K2 group will take two 45-mcg vitamin K2 softgel capsules every day for 8 weeks.

Dietary Supplement: High-Dose Vitamin K2 (menaquinone-7; 90 mcg/d)

Interventions

Placebo-ControlDIETARY_SUPPLEMENT

two placebo softgel capsules per day (for 8 weeks) containing no vitamin K2 (menaquinone-7)

Placebo-Control

one 45-mcg vitamin K2 (menaquinone-7) softgel capsule per day and one placebo softgel per day (containing no menaquinone-7) for 8 weeks

Also known as: menaquinone-7
Low-Dose Vitamin K2 (45-mcg/d)

two 45-mcg vitamin K2 (menaquinone-7) softgel capsules per day for 8 weeks

Also known as: menaquinone-7
High-Dose Vitamin K2 (90-mcg/d)

Eligibility Criteria

Age8 Years - 17 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17)

You may qualify if:

  • Age 8 to 17 years
  • Body mass index equal to or greater than 85th percentile for age and sex
  • Subject and parent/guardian understands the study protocol and agrees to comply with it
  • Informed Consent Form signed by the parent/guardian and assent signed by the subject

You may not qualify if:

  • Subjects using vitamin supplements containing vitamin k
  • Subjects with (a history of) metabolic or gastrointestinal diseases including hepatic disorders
  • Subjects presenting chronic degenerative and/or inflammatory diseases
  • Subjects receiving systemic treatment or topical treatment likely to interfere with evaluation of the study parameters (salicylates, antibiotics)
  • Subjects receiving corticosteroid treatment
  • Subjects using oral anticoagulants
  • Subjects with a history of soy allergy
  • Subjects who have participated in a clinical study more recently than one month before the current study

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Medical College of Georgia; Augusta University

Augusta, Georgia, 30912, United States

Location

Related Publications (1)

  • Douthit MK, Fain ME, Nguyen JT, Williams CF, Jasti AH, Gutin B, Pollock NK. Phylloquinone Intake Is Associated with Cardiac Structure and Function in Adolescents. J Nutr. 2017 Oct 1;147(10):1960-1967. doi: 10.3945/jn.117.253666.

    PMID: 28794209BACKGROUND

MeSH Terms

Conditions

ObesityInsulin ResistanceNutritional and Metabolic DiseasesHyperlipidemiasHyperglycemiaCardiovascular DiseasesMetabolic Diseases

Interventions

Vitamin K 2menaquinone 7

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsHyperinsulinismGlucose Metabolism DisordersDyslipidemiasLipid Metabolism Disorders

Intervention Hierarchy (Ancestors)

Vitamin KNaphthoquinonesNaphthalenesPolycyclic Aromatic HydrocarbonsHydrocarbons, AromaticHydrocarbons, CyclicHydrocarbonsOrganic ChemicalsPhytolDiterpenesTerpenesQuinonesPolycyclic Compounds

Study Officials

  • Norman K Pollock, PhD

    Department of Pediatrics, Medical College of Georgia, Augusta University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

October 11, 2016

First Posted

November 9, 2016

Study Start

October 1, 2016

Primary Completion

December 1, 2020

Study Completion

December 30, 2020

Last Updated

November 20, 2019

Record last verified: 2019-11

Data Sharing

IPD Sharing
Will share

1. Approximate date of when the data will be shared? 2019-06-28 2. Where will the data be made available? The de-identified data will be made available for research purposes only by contacting the principal investigator. 3. Please explain any limits to data sharing that might be required. Even though the final research data will be stripped of identifiers prior to release for sharing, the investigators believe that there remains the possibility of deductive disclosure of subjects with unusual characteristics. Thus, the investigators will make the data available to users only under a data-sharing agreement that provides for: (1) a commitment to using the data only for research purposes and not to identify any individual participant; (2) a commitment to securing the data using appropriate computer technology; and (3) a commitment to destroying or returning the data after analyses are completed.

Locations