Antioxidants Combined With Cryotherapy on Inflammatory Response After Resistance Exercise in Untrained Volunteers

NCT02902315 | Completed

• 1 other identifier: UFSantaMaria2
• Study Type: interventional
• Target: 26
• Locations: 1 country
• Sites: 1

Brief Summary

Acutely resistance exercise induces inflammatory responses and leukocytosis arising of oxidative stress, that clinically manifested by pain and/or delayed onset muscle soreness (DOMS). Beginners in resistance exercise programs are more vulnerable to the effects of oxidative stress as they exhibit lower antioxidant capacity, greater lipid peroxidation and present increased perception of pain after exercises that may lead to abandonment the exercises practice. Vitamins C and E are exogenous antioxidants which are able to prevent damages caused by oxidative stress. Cryotherapy decreases temperature and reduced generation of reactive oxygen species. The aim of the present research are to investigate the effects of the concomitant of vitamins and of cryotherapy on leukocytosis, inflammatory markers, oxidative stress parameters and pain in untrained individuals submitted to a resistance exercise session.

Conditions

Inflammation; Oxidative Stress; Pain

Keywords

Muscle soreness; Free radicals; Ascorbic acid; Alpha-Tocopherol; Cryotherapy; Exercise tolerance; Resistance training

Outcome Measures

Primary Outcomes (1)

• Delayed onset muscle soreness (points)

  Twenty-four hours after the exercise session, the pain or delayed onset muscle soreness (DOMS) was evaluated by visual analog scale (0 - 10 points).

  Assessed 24 hours after resistance exercise session

Secondary Outcomes (11)

• Erythrocytes (/ mm3)

  Baseline (before), and at 0, 30 and 120 minutes after exercises.

• Platelets (/ mm3)

  Baseline (before), and at 0, 30 and 120 minutes after exercises.

• Leukogram (/ mm3)

  Baseline (before), and at 0, 30 and 120 minutes after exercises.

• C reactive protein (mg/dL)

  Baseline (before), and at 0, 30 and 120 minutes after exercises.

• Creatine kinase (U/L)

  Baseline (before), and at 0, 30 and 120 minutes after exercises.

Study Arms (4)

1ª Session of resistance exercises

ACTIVE COMPARATOR

Exercise session are based on previous studies (TEIXEIRA et al., 2012; TEIXEIRA et al., 2014) and will be randomized according to the sequence of exercises (extensor bench, squat and leg press) and the interventions by drawing sealed brown envelopes. Forty minutes before basal blood collection and of the exercise session the volunteers will receive placebo (two pills wheat flour) and the remaining procedures will be conserved. The exercise sessions were comprised of four series of 10 maximum repetitions, with an interval of one minute between series and two minutes between exercises. Before the 10 maximum repetitions test and data collection, standard instructions will be given concerning the experimental procedure and execution technique of the exercises.

Other: Cryotherapy; Other: Vitamins C and E; Other: Vitamins C and E associated with cryotherapy; Other: Placebo

2ª Session of resistance exercises

ACTIVE COMPARATOR

Exercise session are based on previous studies (TEIXEIRA et al., 2012; TEIXEIRA et al., 2014) and will be randomized according to the sequence of exercises (extensor bench, squat and leg press) and the interventions by drawing sealed brown envelopes. Forty minutes before basal blood collection and of the exercise session the volunteers will receive placebo (two pills wheat flour) and the remaining procedures will be conserved. The exercise sessions were comprised of four series of 10 maximum repetitions, with an interval of one minute between series and two minutes between exercises. Before the 10 maximum repetitions test and data collection, standard instructions will be given concerning the experimental procedure and execution technique of the exercises.

Other: Cryotherapy; Other: Vitamins C and E; Other: Vitamins C and E associated with cryotherapy; Other: Placebo

3ª Session of resistance exercises

ACTIVE COMPARATOR

Exercise session are based on previous studies (TEIXEIRA et al., 2012; TEIXEIRA et al., 2014) and will be randomized according to the sequence of exercises (extensor bench, squat and leg press) and the interventions by drawing sealed brown envelopes. Forty minutes before basal blood collection and of the exercise session the volunteers will receive placebo (two pills wheat flour) and the remaining procedures will be conserved. The exercise sessions were comprised of four series of 10 maximum repetitions, with an interval of one minute between series and two minutes between exercises. Before the 10 maximum repetitions test and data collection, standard instructions will be given concerning the experimental procedure and execution technique of the exercises.

Other: Cryotherapy; Other: Vitamins C and E; Other: Vitamins C and E associated with cryotherapy; Other: Placebo

4ª Session of resistance exercises

ACTIVE COMPARATOR

Exercise session are based on previous studies (TEIXEIRA et al., 2012; TEIXEIRA et al., 2014) and will be randomized according to the sequence of exercises (extensor bench, squat and leg press) and the interventions by drawing sealed brown envelopes. Forty minutes before basal blood collection and of the exercise session the volunteers will receive placebo (two pills wheat flour) and the remaining procedures will be conserved. The exercise sessions were comprised of four series of 10 maximum repetitions, with an interval of one minute between series and two minutes between exercises. Before the 10 maximum repetitions test and data collection, standard instructions will be given concerning the experimental procedure and execution technique of the exercises.

Other: Cryotherapy; Other: Vitamins C and E; Other: Vitamins C and E associated with cryotherapy; Other: Placebo

Interventions

Cryotherapy OTHER

The intervention will consist of the application of hypothermia by immersion of the lower limbs of water on individuals to 15ºC for a period of 10 minutes immediately after the exercise protocol.

Also known as: Hypothermia

1ª Session of resistance exercises; 2ª Session of resistance exercises; 3ª Session of resistance exercises; 4ª Session of resistance exercises

Vitamins C and E OTHER

The session of resistance exercises with intervention will be based on the supplementation with vitamin C (1000 mg) and vitamin E (800 IU) by oral intake (100mL of water) 40 minutes before basal blood collection.

Also known as: Ascorbic acid, alfa-tocopherol

1ª Session of resistance exercises; 2ª Session of resistance exercises; 3ª Session of resistance exercises; 4ª Session of resistance exercises

Vitamins C and E associated with cryotherapy OTHER

The session of resistance exercises with intervention will be based on the supplementation with vitamin C (1000 mg) and vitamin E (800 IU) by oral intake (100mL of water) 40 minutes before basal blood collection. The intervention will consist of the application of hypothermia by immersion of the lower limbs of water on individuals to 15ºC for a period of 10 minutes immediately after the exercise protocol.

Also known as: Vitamins C and E, Hypothermia

1ª Session of resistance exercises; 2ª Session of resistance exercises; 3ª Session of resistance exercises; 4ª Session of resistance exercises

Placebo OTHER

The volunteers from placebo exercise session will receive two pills (containing wheat flour) and the remaining procedures were conserved.

Also known as: Control

1ª Session of resistance exercises; 2ª Session of resistance exercises; 3ª Session of resistance exercises; 4ª Session of resistance exercises

Eligibility Criteria

• Age: 20 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age between 20 and 35 years, body mass index (MBI: kg/m2) lower than 30, non-smokers, did not practice physical and/or regular physical exercises (less than two times per week), presented no previous diagnosis of chronic diseases (rheumatic, cardiovascular, metabolic, neurological, oncologic, immunological or hematologic disorders), is not engaged in any diet programs and is not making use of medication.

You may not qualify if:

• Inflammatory response (ultrasensitive C reactive protein \>3 mg/dL), hyperglycemia (\>100 mg/dL), leukocytosis, hyperthermia (\>38ºC), changes in the systemic blood pressure (\>140/90 mmHg) and/or any symptom of pain or discomfort

Sponsors & Collaborators

• Universidade Federal de Santa Maria: lead

Study Sites (1)

Universidade Federal do Rio Grande

Rio Grande, Rio Grande do Sul, 96200-190, Brazil

Location

Related Publications (16)

• Carvalho N, Puntel G, Correa P, Gubert P, Amaral G, Morais J, Royes L, da Rocha J, Soares F. Protective effects of therapeutic cold and heat against the oxidative damage induced by a muscle strain injury in rats. J Sports Sci. 2010 Jul;28(9):923-35. doi: 10.1080/02640414.2010.481722.

  PMID: 20544483 BACKGROUND

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  PMID: 16858479 BACKGROUND

• Bryer SC, Goldfarb AH. Effect of high dose vitamin C supplementation on muscle soreness, damage, function, and oxidative stress to eccentric exercise. Int J Sport Nutr Exerc Metab. 2006 Jun;16(3):270-80. doi: 10.1123/ijsnem.16.3.270.

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  PMID: 18379219 BACKGROUND

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  PMID: 11080266 BACKGROUND

• Finaud J, Lac G, Filaire E. Oxidative stress : relationship with exercise and training. Sports Med. 2006;36(4):327-58. doi: 10.2165/00007256-200636040-00004.

  PMID: 16573358 BACKGROUND

• Lewis PB, Ruby D, Bush-Joseph CA. Muscle soreness and delayed-onset muscle soreness. Clin Sports Med. 2012 Apr;31(2):255-62. doi: 10.1016/j.csm.2011.09.009. Epub 2011 Nov 23.

  PMID: 22341015 BACKGROUND

• Nakajima T, Kurano M, Hasegawa T, Takano H, Iida H, Yasuda T, Fukuda T, Madarame H, Uno K, Meguro K, Shiga T, Sagara M, Nagata T, Maemura K, Hirata Y, Yamasoba T, Nagai R. Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise. Eur J Appl Physiol. 2010 Nov;110(5):905-13. doi: 10.1007/s00421-010-1572-x. Epub 2010 Jul 17.

  PMID: 20640440 BACKGROUND

• Rietjens SJ, Beelen M, Koopman R, VAN Loon LJ, Bast A, Haenen GR. A single session of resistance exercise induces oxidative damage in untrained men. Med Sci Sports Exerc. 2007 Dec;39(12):2145-51. doi: 10.1249/mss.0b013e318157936d.

  PMID: 18046185 BACKGROUND

• Silva LA, Pinho CA, Silveira PC, Tuon T, De Souza CT, Dal-Pizzol F, Pinho RA. Vitamin E supplementation decreases muscular and oxidative damage but not inflammatory response induced by eccentric contraction. J Physiol Sci. 2010 Jan;60(1):51-7. doi: 10.1007/s12576-009-0065-3. Epub 2009 Oct 27.

  PMID: 19859781 BACKGROUND

• Teixeira ADO, Franco OS, Borges MM, et al.. The importance of adjustments for changes in plasma volume in the interpretation of hematological and inflmmatory responses after resistance exercise. J Exerc Physiol 17:72-83, 2014.

  BACKGROUND

• Teixeira ADO, Paulitsch FDS, Umpierre MDM, et al.. Inflammatory response after session of resistance exercises in untrained volunteers. Acta Sci Heal Sci 37:31-39, 2015. doi: 10.4025/actascihealthsci.v37i1.24149

  BACKGROUND

• Schaser KD, Disch AC, Stover JF, Lauffer A, Bail HJ, Mittlmeier T. Prolonged superficial local cryotherapy attenuates microcirculatory impairment, regional inflammation, and muscle necrosis after closed soft tissue injury in rats. Am J Sports Med. 2007 Jan;35(1):93-102. doi: 10.1177/0363546506294569. Epub 2006 Dec 1.

  PMID: 17197574 BACKGROUND

• Traber MG, Stevens JF. Vitamins C and E: beneficial effects from a mechanistic perspective. Free Radic Biol Med. 2011 Sep 1;51(5):1000-13. doi: 10.1016/j.freeradbiomed.2011.05.017. Epub 2011 May 25.

  PMID: 21664268 BACKGROUND

• Wilcock IM, Cronin JB, Hing WA. Physiological response to water immersion: a method for sport recovery? Sports Med. 2006;36(9):747-65. doi: 10.2165/00007256-200636090-00003.

  PMID: 16937951 BACKGROUND

MeSH Terms

Conditions

Inflammation; Pain; Myalgia

Interventions

Cryotherapy; Ascorbic Acid

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms; Neurologic Manifestations; Signs and Symptoms; Muscular Diseases; Musculoskeletal Diseases; Neuromuscular Diseases; Nervous System Diseases; Musculoskeletal Pain

Intervention Hierarchy (Ancestors)

Therapeutics; Sugar Acids; Acids, Acyclic; Carboxylic Acids; Organic Chemicals; Hydroxy Acids; Carbohydrates

Study Officials

• Luis U Signori, PhD

  Universidade Federal de Santa Maria

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: PREVENTION
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD

Study Record Dates

• First Submitted: August 19, 2016
• First Posted: September 15, 2016
• Study Start: January 4, 2017
• Primary Completion: July 10, 2017
• Study Completion: July 12, 2017
• Last Updated: July 17, 2017

Record last verified: 2017-07

Data Sharing

• IPD Sharing: Will not share

Locations

BR (1)