Factors That Influence Adipose Derived Regenerative Cells' Yield and Viability

NCT02651233 | Completed

• 1 other identifier: CCSerbia
• Study Type: observational
• Target: 46
• Locations: 0 countries
• Sites: N/A

Brief Summary

Human adipose tissue has been shown to contain a group of cells that possess extensive proliferative capacity and the ability to differentiate into multiple cell lineages. Cells isolated from the fatty portion are termed processed lipoaspirate cells that contain adipose-derived regenerative cells (ADRCs). ADRCs are most conveniently extracted from tissue during an elective cosmetic liposuction procedure but may also be obtained from resected adipose tissue. This study is aimed to verify if there are differences between the number and viability of cells obtained from patients with different age, sex, Body Mass Index (BMI), smoking and physical activity, within the different amount of processed fat.

Conditions

Problem of Aging; Smoking; Physical Activity; Gender

Outcome Measures

Primary Outcomes (1)

• Body Mass Index (Calculated in kg/m2)

  Calculated in kg/m2

  1h before the surgery

Secondary Outcomes (2)

• ADRCs yield (Calculated in number of cells per gram of tissue)

  2h after the surgery

• ADRCs viability (Calculated in percentage)

  2h after the surgery

Interventions

ADRCs therapy OTHER

Patients are submitted to manual liposuction and extraction of ADRCS is done with closed system. All patients were candidates for subcutaneous transplantation of autologous cell enriched adipose tissue for different indications.

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

All patients were healthy individuals, both genders with request for subcutaneous transplantation of autologous cell enriched adipose tissue for different indications

Sponsors & Collaborators

• Clinical Centre of Serbia: lead

Related Publications (3)

• Buschmann J, Gao S, Harter L, Hemmi S, Welti M, Werner CM, Calcagni M, Cinelli P, Wanner GA. Yield and proliferation rate of adipose-derived stromal cells as a function of age, body mass index and harvest site-increasing the yield by use of adherent and supernatant fractions? Cytotherapy. 2013 Sep;15(9):1098-105. doi: 10.1016/j.jcyt.2013.04.009. Epub 2013 Jun 22.

  PMID: 23800730 RESULT

• Mojallal A, Lequeux C, Shipkov C, Duclos A, Braye F, Rohrich R, Brown S, Damour O. Influence of age and body mass index on the yield and proliferation capacity of adipose-derived stem cells. Aesthetic Plast Surg. 2011 Dec;35(6):1097-105. doi: 10.1007/s00266-011-9743-7. Epub 2011 May 26.

  PMID: 21614659 RESULT

• Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, Tosca MC, Gaetani P, Klinger M, Villani S, Ferretti VV, Vigo D, Torre ML. Nonexpanded mesenchymal stem cells for regenerative medicine: yield in stromal vascular fraction from adipose tissues. Tissue Eng Part C Methods. 2010 Dec;16(6):1515-21. doi: 10.1089/ten.TEC.2010.0214. Epub 2010 Sep 6.

  PMID: 20486782 RESULT

MeSH Terms

Conditions

Smoking; Motor Activity; Coitus

Condition Hierarchy (Ancestors)

Behavior; Sexual Behavior

Study Officials

• Katarina Andjelkov, MD PhD

  BelPrime Clinic

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Clinical professor, principal investigator

Study Record Dates

• First Submitted: January 2, 2016
• First Posted: January 8, 2016
• Study Start: March 1, 2014
• Primary Completion: April 1, 2014
• Study Completion: April 1, 2015
• Last Updated: January 8, 2016

Record last verified: 2016-01

Data Sharing

• IPD Sharing: Will not share