The Effects of Various Cooking Oils on Health Related Biomarkers in Healthy Subjects
1 other identifier
interventional
17
0 countries
N/A
Brief Summary
Fats and oils play important roles in maintaining human nutrition and health through providing energy, essential fatty acids, and acting as modulators of many biological processes (signal transduction, immunity and inflammation). Due to differences in the fatty acid composition and content of antioxidants of individual cooking oils, the degree of oxidative and thermolytic reactions may vary oil by oil. It is lack of human feeding study to investigate the molecular mechanisms on how and which deep-fried oil exerts its adverse effects. The investigators are also lack of biomarkers for monitoring deep-fried oil exposure. Therefore, the purpose of this study is to compare how human body responds differently to several popular uncooked and deep-fried oils with varied fatty acid compositions with respect of oxylipin profile, inflammatory markers, non-targeted metabolomics, and transcriptomics. The investigators will recruit 20 volunteers, provided them once a week the milk shakes prepared from 60g of olive oil, soybean oil, palm oil, camellia oil, tallow (butter), and deep-fried oils of the last 4, respectively; in comparison with a no-fat milk shake control. The experiments lasted for 10 weeks.。Each time; serum, plasma, whole blood and urine samples were collected at baseline, after 2 hours, and after 4 hours. The investigators anticipate to find biomarker(s) for deep-frying, and contribute to the understanding of molecular mechanisms on how deep-fried oils exert adverse effects toward health through integrative omics or so-called system biology approaches.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable
Started Sep 2013
Typical duration for not_applicable
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
September 1, 2013
CompletedPrimary Completion
Last participant's last visit for primary outcome
September 1, 2015
CompletedStudy Completion
Last participant's last visit for all outcomes
September 1, 2015
CompletedFirst Submitted
Initial submission to the registry
September 15, 2015
CompletedFirst Posted
Study publicly available on registry
November 6, 2015
CompletedNovember 6, 2015
October 1, 2015
2 years
September 15, 2015
November 5, 2015
Conditions
Outcome Measures
Primary Outcomes (1)
The changs of metabolomics profile between before and after oil consumption
This is a metabolomics study. We measured retention time (min), mass and abundance of serum metabolites with LC-QTOF at 3 time points. Then, we compared the abundance of metabolites between different oil consumption time ( before and after ) and assessed the health effects of cooking oils.
baseline to 2 hours and 4 hours after oil consumption
Study Arms (10)
fat-free milkshake
PLACEBO COMPARATOROlive oil
ACTIVE COMPARATORsoybean oil
EXPERIMENTALfried soybean oil
EXPERIMENTALpalm oil
EXPERIMENTALfried palm oil
EXPERIMENTALcamellia oil
EXPERIMENTALfried camellia oil
EXPERIMENTALtallow
EXPERIMENTALfried tallow
EXPERIMENTALInterventions
participants drinks the milk shakes prepared from 60g of fried soybean oil
participants drinks the milk shakes prepared from 60g of fried palm oil
participants drinks the milk shakes prepared from 60g of fried camellia oil
Eligibility Criteria
You may qualify if:
- Non-smoking, non-drinker, usual dietary intake
- BMI : 18.5\~27 kg/m2
You may not qualify if:
- Over the past two weeks have suffered from acute illness.
- None of the subjects was taking any supplemental vitamins, antioxidants or medication at that time.
- Taking steroids or non-steroidal anti-inflammatory drug, such as Aspirin or Panadol in the past one week.
- Cancer or other severe diseases was diagnosed.
Contact the study team to confirm eligibility.
Sponsors & Collaborators
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Wen-Harn Pan, Ph.D.
Institute of Biomedical Sciences, Academia Sinica
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- NON RANDOMIZED
- Masking
- DOUBLE
- Who Masked
- PARTICIPANT, CARE PROVIDER
- Purpose
- BASIC SCIENCE
- Intervention Model
- CROSSOVER
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Institute of Biomedical Sciences
Study Record Dates
First Submitted
September 15, 2015
First Posted
November 6, 2015
Study Start
September 1, 2013
Primary Completion
September 1, 2015
Study Completion
September 1, 2015
Last Updated
November 6, 2015
Record last verified: 2015-10