NCT02370277

Brief Summary

This pilot research trial studies the effects of chemotherapy on intestinal bacteria/organisms (microbiota) in patients newly diagnosed with breast cancer. Change in intestinal microbiota may be associated with weight gain in patients treated with chemotherapy. Weight gain has been also associated with cancer recurrence. Examining the types and quantity of bacterial composition in the stool of breast cancer patients treated with chemotherapy may help determine whether body weight and composition are associated with changes in the intestinal microbiota and allow doctors to plan better treatment to prevent weight gain and possibly disease recurrence.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
36

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Dec 2014

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

December 16, 2014

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

February 17, 2015

Completed
7 days until next milestone

First Posted

Study publicly available on registry

February 24, 2015

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 15, 2016

Completed
2.2 years until next milestone

Study Completion

Last participant's last visit for all outcomes

August 22, 2018

Completed
Last Updated

June 18, 2019

Status Verified

June 1, 2019

Enrollment Period

1.5 years

First QC Date

February 17, 2015

Last Update Submit

June 17, 2019

Conditions

Outcome Measures

Primary Outcomes (1)

  • Change in intestinal microbiota (composition, abundance, and diversity)

    Paired sample t-test will be used to determine whether there are significant changes in microbiota diversity (as measured by the number of taxonomic groups) between pre-treatment levels and 1 and 4 months after the final chemotherapy treatment. Mixed-model linear regression on repeated measures will be used to concurrently adjust for physical activity, dietary intakes, and other factors.

    Baseline to 4 months after final adjuvant (or neoadjuvant) chemotherapy course

Secondary Outcomes (3)

  • Change in body composition based on dual-energy x-ray absorptiometry (DEXA)

    Baseline to 4 months after last chemotherapy

  • Baseline estradiol and estrone levels

    Baseline

  • Change in estrogen levels

    Baseline to up to 4 months after last chemotherapy

Study Arms (3)

Group A (stool collection after adjuvant chemotherapy)

Patients undergo collection of stool samples at baseline (before surgery), at 1 week before initiation of adjuvant chemotherapy (after surgery), and at 1 and 4 months after completion of adjuvant chemotherapy.

Other: Cytology Specimen Collection ProcedureOther: Laboratory Biomarker AnalysisOther: Questionnaire Administration

Group B (stool collection after adjuvant chemotherapy)

Patients undergo collection of stool samples at baseline (after surgery), at 1 week before initiation of adjuvant chemotherapy, and at 1 and 4 months after completion of adjuvant chemotherapy.

Other: Cytology Specimen Collection ProcedureOther: Laboratory Biomarker AnalysisOther: Questionnaire Administration

Group C (stool collection after neoadjuvant chemotherapy)

Patients undergo collection of stool samples at baseline, 1 month after completion of neoadjuvant chemotherapy (before surgery), and at 1 and 4 months after surgery

Other: Cytology Specimen Collection ProcedureOther: Laboratory Biomarker AnalysisOther: Questionnaire Administration

Interventions

Undergo collection of stool samples

Also known as: Cytologic Sampling
Group A (stool collection after adjuvant chemotherapy)Group B (stool collection after adjuvant chemotherapy)Group C (stool collection after neoadjuvant chemotherapy)

Correlative studies

Group A (stool collection after adjuvant chemotherapy)Group B (stool collection after adjuvant chemotherapy)Group C (stool collection after neoadjuvant chemotherapy)

Ancillary studies

Group A (stool collection after adjuvant chemotherapy)Group B (stool collection after adjuvant chemotherapy)Group C (stool collection after neoadjuvant chemotherapy)

Eligibility Criteria

Age18 Years+
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients with newly diagnosed breast cancer will be recruited from the Norris Comprehensive Cancer Center and the Los Angeles County + University of Southern California Medical Center.

You may qualify if:

  • Newly diagnosed ductal carcinoma in situ or invasive breast cancer (stage I, stage IIA, stage IIB, and stage IIIA breast cancer)
  • A treatment group including surgery followed by standard treatment of adjuvant chemotherapy such as doxorubicin and cyclophosphamide followed by paclitaxel, docetaxel and cyclophosphamide, or a treatment group including neoadjuvant chemotherapy followed by surgery at the Norris or LAC+USC Medical Center
  • Women of child-bearing potential agree to pregnancy test to confirm she is not pregnant
  • Provide informed consent

You may not qualify if:

  • Metastatic, recurrent, synchronous or metachronous breast cancer
  • History of other cancers (other than non-melanoma skin cancer)
  • History of autoimmune celiac or inflammatory bowel disease
  • Past bariatric surgery
  • Current or recent pregnancy or nursing (within past 12 months)
  • Past treatment with chemotherapy
  • Recent use (within past month) of more than 3 days of antibiotics use
  • Current use of probiotic supplements

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

USC Norris Comprehensive Cancer Center

Los Angeles, California, 90033, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Stool, blood, and urine

MeSH Terms

Conditions

Carcinoma, Intraductal, NoninfiltratingBreast Neoplasms

Condition Hierarchy (Ancestors)

AdenocarcinomaCarcinomaNeoplasms, Glandular and EpithelialNeoplasms by Histologic TypeNeoplasmsBreast Carcinoma In SituCarcinoma in SituNeoplasms, Ductal, Lobular, and MedullaryNeoplasms by SiteBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Study Officials

  • Anna Wu

    University of Southern California

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

February 17, 2015

First Posted

February 24, 2015

Study Start

December 16, 2014

Primary Completion

June 15, 2016

Study Completion

August 22, 2018

Last Updated

June 18, 2019

Record last verified: 2019-06

Locations