NCT02215343

Brief Summary

The main objective of this study is to investigate in detail how a high-(prebiotic)fibre diet and a high-PUFA diet affect the gut microbiota composition in a metabolic challenged population, and if the diet-induced modulation of the gut microbiota mediates changes in metabolic risk markers. Intake of both experimental diets over 4 weeks are expected to induce beneficial changes in the gut microbiota composition and to affect markers for insulin sensitivity, lipid metabolism and inflammation. The investigators hypothesize that the effect of both interventions on the metabolic risk markers will be partly mediated by the diet-induced changes in the gut microbiota composition.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
30

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Aug 2014

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 15, 2014

Completed
17 days until next milestone

Study Start

First participant enrolled

August 1, 2014

Completed
12 days until next milestone

First Posted

Study publicly available on registry

August 13, 2014

Completed
10 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2015

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2015

Completed
Last Updated

May 20, 2020

Status Verified

May 1, 2020

Enrollment Period

10 months

First QC Date

July 15, 2014

Last Update Submit

May 18, 2020

Conditions

Outcome Measures

Primary Outcomes (1)

  • Change in gut microbiota composition

    Gut microbiota composition will be analyzed by use of fecal samples.

    Week 0, 4, 8, 12

Secondary Outcomes (6)

  • Change in markers for insulin sensitivity

    Week 0, 4, 8, 12

  • Change in markers for lipid metabolism

    Week 0, 4, 8, 12

  • Change in markers for inflammation

    Week 0, 4, 8, 12

  • Faecal SCFA and bile acid composition

    Week 0, 4, 8, 12

  • Lipidomics

    Week 0, 4, 8, 12

  • +1 more secondary outcomes

Other Outcomes (3)

  • Change in energy expenditure

    Week 0, 4, 8, 12

  • Gene expression

    Week 0, 4, 8, 12

  • Compliances markers

    Week 0, 4, 8, 12

Study Arms (2)

High fibre diet (wheat bran extract)

EXPERIMENTAL
Dietary Supplement: Wheat bran extract

High PUFA diet (fish oil supplement)

EXPERIMENTAL
Dietary Supplement: Fish oil

Interventions

Wheat bran extractDIETARY_SUPPLEMENT

Patients will be provided with 15g of wheat bran extract (WBE) (Cargill R\&D Centre Europe).

High fibre diet (wheat bran extract)
Fish oilDIETARY_SUPPLEMENT

Patients will be provided with a fish oil supplement (capsules), containing 3-4g of N-3 fatty acids (Axellus A/S, Ishøj, Denmark).

High PUFA diet (fish oil supplement)

Eligibility Criteria

Age18 Years - 60 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • BMI: 25-40 kg/m2
  • Non-smoking
  • Indices of the Metabolic Syndrome defined by the International Diabetes Federation (IDF 2006):
  • Central obesity (waist circumference; men: ≥94 cm and women: ≥80 cm) + any one of the following; raised triglyceride level (≥1.7 mmol/L), reduced HDL cholesterol (men: \<1.03 mmol/L, women: \<1.29 mmol/L), raised BP (systolic ≥130 mm Hg or diastolic ≥85 mm Hg) or raised fasting plasma glucose (≥5.6 mmol/L).

You may not qualify if:

  • Use of antibiotics
  • Weight change \>3 kg 2 months prior to study start
  • Intensive physical training/ elite athlete
  • Medication for dyslipidemia, type 2 diabetes or elevated blood pressure
  • Dietary supplements with pro/prebiotics, dietary fibre or fish oil supplement
  • Lactation, pregnancy or planning of pregnancy during the study
  • Gastro intestinal and liver disorders
  • Chronic inflammatory disorders
  • Diagnosed psychiatric disorder including treatment required depression
  • Surgical treatment of obesity and abdominal surgery

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Nutrition, Exercise and Sports, University of Copenhagen

Frederiksberg, 1958, Denmark

Location

Related Publications (2)

  • Benitez-Paez A, Kjolbaek L, Gomez Del Pulgar EM, Brahe LK, Astrup A, Matysik S, Schott HF, Krautbauer S, Liebisch G, Boberska J, Claus S, Rampelli S, Brigidi P, Larsen LH, Sanz Y. A Multi-omics Approach to Unraveling the Microbiome-Mediated Effects of Arabinoxylan Oligosaccharides in Overweight Humans. mSystems. 2019 May 28;4(4):e00209-19. doi: 10.1128/mSystems.00209-19.

  • Kjolbaek L, Benitez-Paez A, Gomez Del Pulgar EM, Brahe LK, Liebisch G, Matysik S, Rampelli S, Vermeiren J, Brigidi P, Larsen LH, Astrup A, Sanz Y. Arabinoxylan oligosaccharides and polyunsaturated fatty acid effects on gut microbiota and metabolic markers in overweight individuals with signs of metabolic syndrome: A randomized cross-over trial. Clin Nutr. 2020 Jan;39(1):67-79. doi: 10.1016/j.clnu.2019.01.012. Epub 2019 Feb 19.

Related Links

MeSH Terms

Conditions

Overweight

Interventions

Dietary FiberFish Oils

Condition Hierarchy (Ancestors)

OvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Dietary CarbohydratesCarbohydratesFoodDiet, Food, and NutritionPhysiological PhenomenaFood and BeveragesOilsLipids

Study Officials

  • Lesli H Larsen, PhD

    University of Copenhagen

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor, Dr.Med.

Study Record Dates

First Submitted

July 15, 2014

First Posted

August 13, 2014

Study Start

August 1, 2014

Primary Completion

June 1, 2015

Study Completion

June 1, 2015

Last Updated

May 20, 2020

Record last verified: 2020-05

Locations