Effects on Immun System of Anesthesia

NCT02056561 | Completed

• 1 other identifier: MESUT-81
• Study Type: observational
• Target: 45
• Locations: 1 country
• Sites: 1

Brief Summary

Introduction and Aim: Desflurane and sevoflurane are frequently used for maintenance of anesthesia and studies have shown that these anesthetics cause a variety of changes in oxidative stress and antioxidative defense mechanisms. The chemical structure of propofol is similar to some free radical consumers such as endogenous vitamin E and butylated hydroxene toluene. This study aims to compare the effects of sevoflurane, desflurane and propofol infusion anesthesia on the oxidant and antioxidant systems of patients undergoing laparoscopic cholecystectomy. Material and Method: Forty-five patients, ASA (American Society of Anesthesiologists) I-II, score between 18-50 years with planned laparoscopic cholecystectomy under general anesthesia were included in the study. Patients were divided into 3 groups as total intravenous anesthesia (TIVA) (group T n: 15), sevoflurane (group S n: 15) and desflurane (group D n: 15). Propofol 2 mg/kg IV, fentanyl 1 mcg/kg IV, vecuronium 0.1 mg/kg IV given to all groups for anesthesia induction. For maintenance of anesthesia group S were ventilated with 2% sevoflurane, 50% air and 50% O2 mix at 6 L/min flow. Group D were given 6% desflurane 50% air and 50% O2 mix at 6 L/min flow. Group T were given propofol infusions of 12 mg/kg/hr for the first 10 minutes, 9 mg/kg/hr for the second 10 minutes and 6 mg/kg/hr after that. Patients were ventilated with 50% air and 50% O2 mix at 6 L/min flow. Before induction and after the operation venous blood samples were taken to evaluate the levels of glutathion peroxidase, total oxidants and antioxidants.

Conditions

Anesthesia

Keywords

Anesthesia; Oxidants; Antioxidants

Outcome Measures

Primary Outcomes (1)

• Levels of glutathion peroxidase, total oxidants and antioxidants.

  Before induction and after the operation venous blood samples were taken to evaluate the levels of glutathion peroxidase, total oxidants and antioxidants.

  24 hours

Study Arms (3)

TIVA (group T n: 15)

Group T were given propofol infusions of 12 mg/kg/hr for the first 10 minutes, 9 mg/kg/hr for the second 10 minutes and 6 mg/kg/hr after that. Patients were ventilated with 50% air and 50% O2 mix at 6 L/min flow.

Sevoflurane (group S n: 15)

Group S were ventilated with 2% sevoflurane, 50% air and 50% O2 mix at 6 L/min flow.

Desflurane (group D n: 15)

Group D cases were given 6% desflurane 50% air and 50% O2 mix at 6 L/min flow.

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

45 patients ASA I-II between 18-50 years with planned laparoscopic cholecystectomy under general anesthetic were included in the study. Patients were divided into 3 groups on the way to surgery; TIVA (group T n: 15), sevoflurane (group S n: 15) and desflurane (group D n: 15).

You may qualify if:

• ASA I-II with patients
• Between 18-50 years
• Planned laparoscopic cholecystectomy under general anesthetic

You may not qualify if:

• Patients with endocrine dysfunction
• blood transfusion in the previous 2 weeks
• signs of infection and inflammation
• history of preoperative medication use
• anemia
• hemorrhage requiring transfusion during the operation were excluded from the study.

Sponsors & Collaborators

• Duzce University: lead

Study Sites (1)

Duzce Univercity Medical Fauculty

Düzce, 81600, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

Before induction and after the operation venous blood samples were taken.

Study Officials

• yavuz demiraran, MD prof

  duzce university medical faculty

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Target Duration: 3 Days
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor Doctor

Study Record Dates

• First Submitted: January 29, 2014
• First Posted: February 6, 2014
• Study Start: January 1, 2010
• Primary Completion: July 1, 2010
• Study Completion: January 1, 2011
• Last Updated: February 6, 2014

Record last verified: 2014-02

Locations

TU (1)