Determination of Adipocytokine in Synovial Fluid of Osteoarthritis Patients

NCT01993342 | Unknown

• 1 other identifier: Joan03
• Study Type: observational
• Target: 35
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to determine the concentration of different adipocytokine (adiponectin, leptin, resistin, visfatin, osteopontin) and an inflammation classic markers as Interleukine 1 beta, tumor necrosis alfa, interleukine 6 and high sensibility reactive c protein in the synovial fluid of knee osteoarthritis patients, and compare with the inflammation detected with ultrasonography evaluation of the joint. In last years, some studies appeared focusing on the role of adipocytokines in osteoarthritis and they correlated adipocytokines with cartilage degradation and x-ray degree; in our study we will focused in the importance of adipocytokines in the inflammatory changes more than in joint destruction.

Conditions

Osteoarthritis

Keywords

adipocytokines; osteoarthritis; inflammation; ultrasonography

Outcome Measures

Primary Outcomes (1)

• Determination of adipocytokine levels (adiponectin, leptin, resistin, visfatin, osteopontin) in synovial fluid

  We have all the synovial fluid freeze, so now we have to determine the levels of everyone.

  We determine adipocytoquines in one month, all patients in the same determination kit

Secondary Outcomes (2)

• Knee inflammation parameters detected by ultrasonography

  three months

• To determine additional classic parameters of inflammation in synovial fluid as Interleukine 1 beta, Interleukine 6 and tumor necrosis factor alfa

  We determine this inflammatory parameters in one month in all patient at the same time

Study Arms (1)

Inflammatory knee osteoarthritis

We recruited patients with osteoarthritis and synovial effusion to diagnostic the effusion and to confirm that this this synovial fluid had mechanical characteristics. Now we are going to determine the adipocytokines and traditional parameters of inflammation in this synovial fluid to correlate it, and we will associate it with the ultrasound explanation of the knee that we have in our database.

Other: arthrocentesis; Other: ultrasonography of the knee

Interventions

arthrocentesis OTHER

The made a diagnostic arthrocentesis to all patient we included

Inflammatory knee osteoarthritis

ultrasonography of the knee OTHER

We explored the affected knee with an ultrasound explanation in order to describe inflammatory changes based on standard examination.

Inflammatory knee osteoarthritis

Eligibility Criteria

• Age: 50 Years - 81 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Patient with knee osteoarthritis and synovial effusion who attende to a Rheumatologist unit for this problem. We made the visit and anonimously we kept the results of examination and ultrasonography in our database and we freezed synovial fluid. Now we will determine the adipocytokines and classic inflammatory parameters in this synovial fluid

You may qualify if:

• aged 50 to 81
• primary osteoarthritis of the knee
• synovial effusion in examination
• patient who have the results in our database

You may not qualify if:

• any other rheumatic conditions as rheumatoid arthritis or another inflammatory disease
• secondary knee osteoarthritis
• history of knee surgery
• any conditions that at investigator criteria exclude the patient from study procedures

Sponsors & Collaborators

• Parc Taulí Hospital Universitari: lead

Study Sites (1)

Hospital Parc Tauli Sabadell

Sabadell, Barcelona, 08208, Spain

RECRUITING

Biospecimen

Retention: SAMPLES WITHOUT DNA

Synovial fluid

MeSH Terms

Conditions

Osteoarthritis; Inflammation

Interventions

Arthrocentesis

Condition Hierarchy (Ancestors)

Arthritis; Joint Diseases; Musculoskeletal Diseases; Rheumatic Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Paracentesis; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Therapeutics; Surgical Procedures, Operative; Investigative Techniques

Study Officials

• Joan Calvet, Dr

  Hospital Parc Tauli

  PRINCIPAL INVESTIGATOR

• Cristobal Orellana, Dr

  Hospital Parc Tauli

  STUDY DIRECTOR

Central Study Contacts

Joan Calvet, Dr

CONTACT

0034 937231010; joan.calvet.fontova@gmail.com

Cristobal Orellana, Dr

CONTACT

0034 937231010; cristobalorellana1@gmail.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr

Study Record Dates

• First Submitted: November 8, 2013
• First Posted: November 25, 2013
• Study Start: October 1, 2013
• Primary Completion: November 1, 2013
• Study Completion: March 1, 2014
• Last Updated: November 25, 2013

Record last verified: 2013-11

Locations

ES (1)