Fructose-induced Intestinal de Novo Lipogenesis

NCT01792089 | Completed

• 1 other identifier: 193/11
• Study Type: observational
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1 Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Conditions

Obesity

Keywords

de novo lipogenesis; fructose; roux-en-Y gastric bypass; post-prandial triglycerides

Outcome Measures

Primary Outcomes (2)

• Intestinal de novo lipogenesis (sub-study 1)

  Measurement of total TG and 13C palmitate in chylomicrons: The sf \>20 fraction of post-prandial blood samples will be prepared, and non-chylomicrons, apoB100-containing lipoprotein will be removes by immuno-affinity chromatography using a specific anti-apoB100 antibody

  0-8-hour post-prandial

• Postprandial exogenous, labelled palmitate concentration (sub-study 2)

  Measurement of total TG and 13C palmitate in chylomicrons of sf \>20 fraction of post-prandial blood samples will be prepared,

  0-8-hour post-prandial

Secondary Outcomes (1)

• post-prandial triglyceride profile (sub-studies 1 and 2

  0-8 hour post-prandial

Study Arms (3)

non-obese healthy subjects

subjects with BMI\<25 and no known disease

Other: fat+protein meal; Other: Fat + protein + 13C fructose meal; Other: Fat+protein+13C fructose+glucose meal; Other: 13C Fat + whey protein; Other: 13C Fat + whey protein + glucose+ glucose meal

post-gastric bypass

post-obese subjects 12-48 months after Roux-en-Y gastric bypass

Other: fat+protein meal; Other: Fat + protein + 13C fructose meal; Other: 13C Fat + whey protein + glucose+ glucose meal

matched control subjects

Nonobese, healthy subjects of similar age, weight, and gender ratio, than post-bypass subjects

Other: fat+protein meal; Other: Fat + protein + 13C fructose meal; Other: 13C Fat + whey protein + glucose+ glucose meal

Interventions

fat+protein meal OTHER

cream+ whey protein

matched control subjects; non-obese healthy subjects; post-gastric bypass

Fat + protein + 13C fructose meal OTHER

cream+ whey protein +13C fructose

matched control subjects; non-obese healthy subjects; post-gastric bypass

Fat+protein+13C fructose+glucose meal OTHER

Cream + whey protein + glucose:13C fructose mixture

non-obese healthy subjects

13C Fat + whey protein OTHER

cream labelled with 13C palmitate + whey protein

non-obese healthy subjects

13C Fat + whey protein + glucose+ glucose meal OTHER

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

matched control subjects; non-obese healthy subjects; post-gastric bypass

Eligibility Criteria

• Age: 25 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

8 non-obese healthy male and female subjects with BMI \<25 8 obese male and female subjects with BMI\> 35 8 post-gastric bypass male and female subjects 8 healthy subjects matched to post-gastric bypass subjects for age, gender and weight

You may qualify if:

• age 18-50 years
• sex male or female
• less than 2 kg body weight change over the past 3 months subjects

You may not qualify if:

• antidiabetic and hypolipemic drugs
• alcohol consumption \>20g/day
• severe eating disorders
• severe psychological problems
• vegetarian diet or other specific diet
• consumption of illicit substances
• pregnancy
• weight gain or weight loss \> 1 kg in the last 3 months

Sponsors & Collaborators

• University of Lausanne: lead

Study Sites (1)

Centre Hospitalier Universitaire Vaudois

Lausanne, Canton of Vaud, Switzerland

Location

Related Publications (1)

• Theytaz F, de Giorgi S, Hodson L, Stefanoni N, Rey V, Schneiter P, Giusti V, Tappy L. Metabolic fate of fructose ingested with and without glucose in a mixed meal. Nutrients. 2014 Jul 15;6(7):2632-49. doi: 10.3390/nu6072632.

  PMID: 25029210 DERIVED

MeSH Terms

Conditions

Obesity

Interventions

CD36 Antigens; Proteins; Whey Proteins

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Platelet Membrane Glycoproteins; Membrane Glycoproteins; Glycoproteins; Glycoconjugates; Carbohydrates; Fatty Acid Transport Proteins; Membrane Transport Proteins; Carrier Proteins; Amino Acids, Peptides, and Proteins; Membrane Proteins; Receptors, Cell Surface; Receptors, Immunologic; Scavenger Receptors, Class B; Receptors, Scavenger; Receptors, LDL; Receptors, Lipoprotein; Milk Proteins; Animal Proteins, Dietary; Dietary Proteins; Whey; Milk; Dairy Products; Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: February 12, 2013
• First Posted: February 15, 2013
• Study Start: February 1, 2013
• Primary Completion: December 1, 2016
• Study Completion: December 1, 2016
• Last Updated: March 25, 2019

Record last verified: 2019-03

Locations

CH (1)