Study the Effect of Oral Zinc Supplementation on Enzymes of Nitric Oxide Pathway

NCT01684059 | Completed

• 1 other identifier: Babil-2
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

The ability of spermatozoa to produce reactive oxygen species (ROS) has been respected since the 1940's. Oxidative stress limits the functional competence of mammalian spermatozoa via lipid peroxidation, the induction of oxidative DNA damage and the formation of protein adducts. Nitric oxide (NO) is a free radical generated from the oxidation of L-arginine to L-citrulline by reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent NO synthases. Several studies suggest that the overproduction of this free radical and the subsequent excessive exposure to oxidative conditions have a potential pathogenetic implication, which due to the reduction of sperm motility. The present study was conducted to study the effect of Zn supplementation on the levels of NO synthase and arginase in semen of patients with asthenozoospmia.

Conditions

Asthenozoospermia

Outcome Measures

Primary Outcomes (1)

• Nitric oxide synthase activity

  at the end of three months

Secondary Outcomes (1)

• Arginase activity

  at the end of three months

Study Arms (1)

Single group

Single group: each participant receive same intervention (zinc sulfate) throughout study (non-randomized)

Dietary Supplement: zinc sulfate

Interventions

zinc sulfate DIETARY_SUPPLEMENT

every participant took two capsules of zinc sulfate per day for three months (each one 220mg)

Single group

Eligibility Criteria

• Age: 27 Years - 35 Years
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

this study includes 60 fertile male partners from couples who had consulted the infertility clinic of babil hospital of maternity (Hilla city/ IRAQ).

You may qualify if:

• the presence of asthenozoospermia in the semen sample.

You may not qualify if:

• the absence of endocrinopathy,
• varicocele, and
• female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels

Sponsors & Collaborators

• Babylon University: lead
• Ministry of Health, Iraq: collaborator

Study Sites (1)

Babylon university/ college of science

Hillah, Hilla, IQ, Iraq

Location

Related Publications (1)

• Hadwan MH, Almashhedy LA, Alsalman AR. Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients. Reprod Biol Endocrinol. 2014 Jan 3;12:1. doi: 10.1186/1477-7827-12-1.

  PMID: 24383664 DERIVED

MeSH Terms

Conditions

Asthenozoospermia

Interventions

Zinc Sulfate

Condition Hierarchy (Ancestors)

Infertility, Male; Genital Diseases, Male; Genital Diseases; Urogenital Diseases; Infertility; Male Urogenital Diseases

Intervention Hierarchy (Ancestors)

Sulfates; Sulfuric Acids; Sulfur Acids; Sulfur Compounds; Inorganic Chemicals; Zinc Compounds

Study Officials

• mahmoud H. hadwan, phD

  babylon university / Iraq

  PRINCIPAL INVESTIGATOR

• Lamia A. Almashhedy, phD

  Babylon university/Iraq

  STUDY CHAIR

• abdulrrazaq S. Alsalman, phD

  Babylon university/Iraq

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: phD

Study Record Dates

• First Submitted: September 9, 2012
• First Posted: September 12, 2012
• Study Start: July 1, 2011
• Primary Completion: July 1, 2012
• Study Completion: July 1, 2012
• Last Updated: September 12, 2012

Record last verified: 2012-09

Locations

IR (1)