Post-exercise Recovery After Dietary Protein Ingestion in Healthy Young Men (Meat-Milk Study)

NCT01578590 | Completed

• 1 other identifier: METC 11-3-088
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Rationale: The consumption of dietary protein immediately after exercise is necessary to maximally stimulate muscle protein synthesis rates (24, 37). Recent work suggests that the type of protein consumed (e.g., animal vs. plant-derived proteins) during post-exercise recovery can affect the amplitude of acute increases in muscle protein synthesis rates (25, 31). Specifically, consumption of bovine milk proteins immediately after a single bout of resistance exercise stimulates muscle protein synthesis rates greater than consumption of an isonitrogenous soy-protein beverage (31, 37). Importantly, consumption of milk promotes greater hypertrophy than soy after resistance training (10). Thus, it is generally assumed that the acute muscle protein synthetic response predicts long-term training outcomes, such as hypertrophy. Currently, a great amount of work has been carried out to study the effects of consuming milk proteins on muscle protein synthesis rates after resistance exercise (5, 7, 26, 32). However, very little is known about the effects of other types of high-quality animal proteins, such as beef, on stimulating post-exercise muscle protein synthesis rates. Further describing the muscle protein synthetic response after consumption of other types of high-quality animal proteins will provide valuable information for individuals with milk allergies, lactose intolerance, or simply a strong dislike of dairy products. Objective: To investigate whether the in vivo post-resistance exercise muscle protein synthetic response is augmented when minced beef is ingested as compared to an isonitrogenous-matched milk protein beverage in healthy young men. Study design: Crossover, randomized Study population: 12 healthy young males (18-35 y). Intervention: Subjects will perform resistance exercise and consume either a piece of meat (135 grams, 35 g of protein) or an isonitrogenous-matched milk protein beverage on two separate test days. In addition, continuous intravenous tracer infusions will be applied, with plasma and muscle samples collected. A two week 'wash-out' period will be included between trials. Main study parameters/endpoints Primary endpoint: Muscle protein synthetic rate, expressed as fractional synthetic rate (FSR). Secondary endpoints: Rate of protein digestion and absorption and whole body protein balance.

Conditions

Skeletal Muscle Hypertrophy

Outcome Measures

Primary Outcomes (1)

• Muscle protein synthetic rate, expressed as fractional synthetic rate (FSR)

  1 day

Secondary Outcomes (1)

• Rate of protein digestion and absorption and whole body protein balance

  1 day

Study Arms (2)

Subjects will consume lean minced meat

EXPERIMENTAL

Subjects will perform resistance exercise and consume a piece of meat (135 grams, 35 g of protein)

Dietary Supplement: Lean minced meat (beef)

Subjects will consume a milk beverage

ACTIVE COMPARATOR

Subjects will perform resistance exercise and consume a milk protein beverage

Dietary Supplement: Lean minced meat (beef)

Interventions

Lean minced meat (beef) DIETARY_SUPPLEMENT

A piece of meat (135 grams, 35 g of protein) or an isonitrogenous-matched milk protein beverage

Also known as: Meat, Milk

Subjects will consume a milk beverage; Subjects will consume lean minced meat

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Males
• Aged between 18-35 years
• Healthy, recreationally active
• BMI \< 25 kg/m2

You may not qualify if:

• Smoking
• Allergies to milk proteins (whey or casein)
• Vegetarians
• Female
• Arthritic conditions
• A history of neuromuscular problems
• Previous participation in amino acid tracer studies
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).

Sponsors & Collaborators

• Maastricht University Medical Center: lead

Study Sites (1)

University of Maastricht

Maastricht, Limburg, 6229 ER, Netherlands

Location

Related Publications (2)

• Gorissen SHM, Trommelen J, Kouw IWK, Holwerda AM, Pennings B, Groen BBL, Wall BT, Churchward-Venne TA, Horstman AMH, Koopman R, Burd NA, Fuchs CJ, Dirks ML, Res PT, Senden JMG, Steijns JMJM, de Groot LCPGM, Verdijk LB, van Loon LJC. Protein Type, Protein Dose, and Age Modulate Dietary Protein Digestion and Phenylalanine Absorption Kinetics and Plasma Phenylalanine Availability in Humans. J Nutr. 2020 Aug 1;150(8):2041-2050. doi: 10.1093/jn/nxaa024.

  PMID: 32069356 DERIVED

• Burd NA, Gorissen SH, van Vliet S, Snijders T, van Loon LJ. Differences in postprandial protein handling after beef compared with milk ingestion during postexercise recovery: a randomized controlled trial. Am J Clin Nutr. 2015 Oct;102(4):828-36. doi: 10.3945/ajcn.114.103184. Epub 2015 Sep 9.

  PMID: 26354539 DERIVED

MeSH Terms

Interventions

Red Meat; Meat; Milk

Intervention Hierarchy (Ancestors)

Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages; Beverages; Dairy Products

Study Officials

• Luc JC van Loon, Prof. Dr.

  Maastricht University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: SUPPORTIVE CARE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: March 15, 2012
• First Posted: April 17, 2012
• Study Start: May 1, 2012
• Primary Completion: August 1, 2012
• Study Completion: August 1, 2012
• Last Updated: December 2, 2014

Record last verified: 2014-11

Locations

NL (1)