Changes in the Distribution of Mitochondria During Oocyte Maturation

NCT01501292 | Unknown DMC

• 1 other identifier: 0095-11-HYMC
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Cytoplasmatic maturation is an important event in the general maturation of of the oocyte. Mitochondria is one of the major organelles involved in the process of cytoplasmatic maturation. The aim of this study is to investigate the presence and distribution of the mitochondria in the cytoplasma of immature oocytes that are retrieved in the routine in-vitro maturation cycle with intracytoplasmatic sperm injection (ICSI). These oocytes will not undergo ICSI because of their immaturity. Usually, they are discarded. In addition to these immature oocytes the investigators shall also investigate the mitochondria in mature oocytes that underwent ICSI but did not become fertilized and are discarded.

Conditions

Infertility

Outcome Measures

Primary Outcomes (1)

• The patterns of mitochondrial distribution within the cytoplasma of oocytes during nuclear maturation

  Identification the different patterns of mitochondrial distribution in correlation with the 3 stages nuclear maturation in human oocytes

  Three months

Study Arms (2)

Immature oocytes (GV, M-I)

Other: Fixation and staining; Other: Observation by laser microscopy

Mature oocytes (M-II)

Other: Fixation and staining; Other: Observation by laser microscopy

Interventions

Fixation and staining OTHER

Fixation and staining by MitiTracker Green FM

Immature oocytes (GV, M-I); Mature oocytes (M-II)

Observation by laser microscopy OTHER

Observation with Laser scanning confocal microscopy

Immature oocytes (GV, M-I); Mature oocytes (M-II)

Eligibility Criteria

• Age: 18 Years - 44 Years
• Sex: female
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Patients undergoing routine in-vitro fertilization with ICSI

You may qualify if:

• Infertile patients needing IVF treatment

You may not qualify if:

• Patients not requiring the ICSI procedure

Sponsors & Collaborators

• Hillel Yaffe Medical Center: lead

Study Sites (1)

Hillel Yaffe Medical Center

Hadera, 38100, Israel

Location

MeSH Terms

Conditions

Infertility

Interventions

Staining and Labeling

Condition Hierarchy (Ancestors)

Genital Diseases; Urogenital Diseases

Intervention Hierarchy (Ancestors)

Histocytological Preparation Techniques; Cytological Techniques; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Histological Techniques; Investigative Techniques

Central Study Contacts

Adrian Ellenbogen, MD

CONTACT

972-50-6246722; ellenbogen@hy.health.gov.il

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER GOV
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 13, 2011
• First Posted: December 29, 2011
• Study Start: January 1, 2012
• Primary Completion: April 1, 2012
• Study Completion: April 1, 2012
• Last Updated: December 29, 2011

Record last verified: 2011-12

Locations

IL (1)