Biomarkers in Blood and Tissue Samples From Patients With Epstein-Barr Virus-Positive Hodgkin Lymphoma

NCT01490801 | Completed

• 3 other identifiers: AHOD12B1COG-AHOD12B1NCI-2012-00089
• Study Type: observational
• Target: 50
• Locations: 0 countries
• Sites: N/A

Brief Summary

RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in blood and tissue samples from patients with Epstein-Barr virus positive Hodgkin lymphoma.

Conditions

Lymphoma; Nonneoplastic Condition

Keywords

childhood Hodgkin lymphoma; childhood mixed cellularity Hodgkin lymphoma; hemophagocytic lymphohistiocytosis; Epstein-Barr virus infection

Outcome Measures

Primary Outcomes (3)

• Presence of germline ITK mutations

• Influence of ITK mutations on total expression levels or intracellular localization of the ITK protein

• Correlation between ITK mutations with specific clinical or histopathological features of HL

Interventions

DNA analysis GENETIC

RNA analysis GENETIC

cytogenetic analysis GENETIC

in situ hybridization GENETIC

mutation analysis GENETIC

polymerase chain reaction GENETIC

western blotting GENETIC

flow cytometry OTHER

immunohistochemistry staining method OTHER

medical chart review OTHER

Eligibility Criteria

• Age: Up to 9 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17)
• Sampling Method: Non-Probability Sample

Study Population

Diagnosis of Hodgkin lymphoma.

DISEASE CHARACTERISTICS: * Diagnosis of Hodgkin lymphoma (HL) meeting the following criteria: * Epstein-Barr virus-positive (EBV+) HL as assessed by positive EBV serology, elevated levels of EBV genome in the blood or tumor tissue following quantitative polymerase chain reaction (PCR) and/or evidence of EBV positivity of pathology samples (EBER+ or LMP+) and, when possible, mixed cellular histology * Young age (\< 10 years) at diagnosis * Presence of hemophagocytic lymphohistiocytosis (HLH) PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Contact the study team to discuss eligibility requirements. They can help determine if this study is right for you.

Sponsors & Collaborators

• Children's Oncology Group: lead
• National Cancer Institute (NCI): collaborator

Biospecimen

Retention: SAMPLES WITH DNA

Blood and Tissue

MeSH Terms

Conditions

Lymphoma; Hodgkin Disease; Lymphohistiocytosis, Hemophagocytic; Epstein-Barr Virus Infections

Interventions

Cytogenetic Analysis; In Situ Hybridization; Polymerase Chain Reaction; Blotting, Western; Flow Cytometry; Immunohistochemistry

Condition Hierarchy (Ancestors)

Neoplasms by Histologic Type; Neoplasms; Lymphoproliferative Disorders; Lymphatic Diseases; Hemic and Lymphatic Diseases; Immunoproliferative Disorders; Immune System Diseases; Histiocytosis, Non-Langerhans-Cell; Histiocytosis; Herpesviridae Infections; DNA Virus Infections; Virus Diseases; Infections; Tumor Virus Infections

Intervention Hierarchy (Ancestors)

Cytological Techniques; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Investigative Techniques; Genetic Techniques; Staining and Labeling; Histocytological Preparation Techniques; Histological Techniques; Nucleic Acid Hybridization; Nucleic Acid Amplification Techniques; Electrophoresis; Chemistry Techniques, Analytical; Electrochemical Techniques; Immunoblotting; Immunoassay; Immunologic Techniques; Molecular Probe Techniques; Cell Separation; Cytophotometry; Fluorometry; Luminescent Measurements; Photometry; Histocytochemistry

Study Officials

• Kim E. Nichols, MD, BA

  Children's Hospital of Philadelphia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: NETWORK
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 9, 2011
• First Posted: December 13, 2011
• Study Start: December 1, 2011
• Primary Completion: May 1, 2016
• Study Completion: May 1, 2016
• Last Updated: May 17, 2016

Record last verified: 2016-05