NCT01424306

Brief Summary

People with chronic low-grade inflammation have a higher risk for certain diseases such as cardiovascular disease or type 2 diabetes. While it is known that obese people are more likely to show signs of low-grade inflammation than lean individuals, it is unclear what causes this inflammation. In the proposed study, the investigators will examine whether the sugar fructose, when consumed in a sweetened beverage, triggers low-grade inflammation in healthy men and women compared with other caloric sweeteners.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
25

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Oct 2011

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 24, 2011

Completed
2 days until next milestone

First Posted

Study publicly available on registry

August 26, 2011

Completed
1 month until next milestone

Study Start

First participant enrolled

October 1, 2011

Completed
2.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2014

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 1, 2014

Completed
3.4 years until next milestone

Results Posted

Study results publicly available

August 14, 2017

Completed
Last Updated

August 14, 2017

Status Verified

July 1, 2017

Enrollment Period

2.5 years

First QC Date

August 24, 2011

Results QC Date

April 19, 2017

Last Update Submit

July 6, 2017

Conditions

Keywords

InflammationIntestinal permeabilityType 2 diabetes mellitusCardiovascular diseaseObesitySweetened beveragesSugarFructoseGlucoseHigh-fructose corn syrup

Outcome Measures

Primary Outcomes (2)

  • Fasting Plasma C-reactive Protein

    The concentration of C-reactive protein in fasting plasma will be measured by high-sensitivity assay at the beginning (day 1) and end (day 9) of each 8-day dietary period.

    Beginning (day 1) and end (day 9) of each diet period.

  • Fasting Plasma Interleukin-6 on Day 9 of Each Diet Period

    The concentration of interleukin-6 in fasting plasma will be measured by high-sensitivity enzyme-linked immunosorbent assay at the end (day 9) of each 8-day dietary period.

    End (day 9) of each diet period

Secondary Outcomes (11)

  • Fasting Plasma Adiponectin

    End (day 9) of each diet period.

  • Mean Daily Calorie Intake

    The mean daily calorie intake during each of the 8-day diet periods will be calculated.

  • Intestinal Permeability, as Assessed by the 5-hour Urinary Lactulose/Mannitol Test

    End (day 9) of each diet period.

  • Fasting Plasma Zonulin Concentrations

    End (day 9) of each diet period.

  • Fasting Plasma Lipopolysaccharide-binding Protein (LBP)

    End (day 9) of each diet period.

  • +6 more secondary outcomes

Study Arms (3)

Fructose-sweetened beverages

EXPERIMENTAL

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a fructose-sweetened beverage for 8 days.

Other: Fructose-sweetened beverages

Glucose-sweetened beverages

EXPERIMENTAL

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a glucose-sweetened beverage for 8 days.

Other: Glucose-sweetened beverages

High-fructose corn syrup-sweetened beverages

EXPERIMENTAL

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a high-fructose corn syrup-sweetened beverage for 8 days.

Other: High-fructose corn syrup-sweetened beverages

Interventions

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a fructose-sweetened beverage for 8 days. The amount of fructose consumed will be 25% of the subject's estimated daily calorie requirement.

Fructose-sweetened beverages

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a glucose-sweetened beverage for 8 days. The amount of glucose consumed will be 25% of the subject's estimated daily calorie requirement.

Glucose-sweetened beverages

In addition to consuming a standardized diet, subjects will be asked to consume 4 servings per day of a high-fructose corn syrup-sweetened beverage for 8 days. The amount of high-fructose corn syrup consumed will be 25% of the subject's estimated daily calorie requirement.

High-fructose corn syrup-sweetened beverages

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age: 18-65 years;
  • BMI 20-40 kg/m2;
  • Weight stable to within 10 pounds for 6 months prior to entering the study, and at their lifetime maximum weight (or within 30 pounds of it; excluding pregnancy);
  • Ability to be admitted for \~30 minutes on three occasions, and \~6 hours on three occasions to the FHCRC Prevention Center;
  • Ability to provide informed written consent;
  • Willingness to consume only food and beverages provided by the Human Nutrition Laboratory of the FHCRC Prevention Center for three periods of 8 days each.

You may not qualify if:

  • Presence or history of chronic inflammatory, autoimmune or metabolic diseases;
  • Presence of phenylketonuria, hereditary fructose intolerance, fructose malabsorption, or malabsorption syndromes;
  • Abuse of alcohol (\>2 drinks per day), smoking, or use of recreational drugs;
  • Current or recent (within three months) intake of medications likely to interfere with study endpoints (insulin, antidiabetics, β-blockers, anabolic steroids, glucocorticosteroids, daily high-dose non-steroidal anti-inflammatory drugs, warfarin, antibiotics, probiotics);
  • Presence of anemia, recent (within 2 months) history of anemia;
  • Anyone not willing or able to eat the provided food;
  • Current or recent (within 12 months) pregnancy or breastfeeding.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Fred Hutchinson Cancer Research Center

Seattle, Washington, 98109, United States

Location

Related Publications (2)

  • Kuzma JN, Cromer G, Hagman DK, Breymeyer KL, Roth CL, Foster-Schubert KE, Holte SE, Callahan HS, Weigle DS, Kratz M. No difference in ad libitum energy intake in healthy men and women consuming beverages sweetened with fructose, glucose, or high-fructose corn syrup: a randomized trial. Am J Clin Nutr. 2015 Dec;102(6):1373-80. doi: 10.3945/ajcn.115.116368. Epub 2015 Nov 4.

    PMID: 26537945BACKGROUND
  • Kuzma JN, Cromer G, Hagman DK, Breymeyer KL, Roth CL, Foster-Schubert KE, Holte SE, Weigle DS, Kratz M. No differential effect of beverages sweetened with fructose, high-fructose corn syrup, or glucose on systemic or adipose tissue inflammation in normal-weight to obese adults: a randomized controlled trial. Am J Clin Nutr. 2016 Aug;104(2):306-14. doi: 10.3945/ajcn.115.129650. Epub 2016 Jun 29.

    PMID: 27357093BACKGROUND

MeSH Terms

Conditions

Diabetes Mellitus, Type 2Cardiovascular DiseasesObesityInflammation

Condition Hierarchy (Ancestors)

Diabetes MellitusGlucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic DiseasesEndocrine System DiseasesOverweightOvernutritionNutrition DisordersBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsPathologic Processes

Results Point of Contact

Title
Mario Kratz
Organization
Fred Hutchinson Cancer Research Center

Study Officials

  • Mario Kratz, MS, PhD

    Fred Hutchinson Cancer Center

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Member

Study Record Dates

First Submitted

August 24, 2011

First Posted

August 26, 2011

Study Start

October 1, 2011

Primary Completion

April 1, 2014

Study Completion

April 1, 2014

Last Updated

August 14, 2017

Results First Posted

August 14, 2017

Record last verified: 2017-07

Locations