Essential Amino Acids Supplementation and Muscle Protein Synthesis
The Effect of Amino Acid Supplementation on Skeletal Muscle Protein Turnover Following Endurance Exercise
2 other identifiers
interventional
23
1 country
2
Brief Summary
The modern warfighter faces numerous physiological challenges including sleep deprivation, sustained intense physical activity, and caloric restriction, the combined effects of which may result in the loss of lean body mass and decreased physical performance. Dietary interventions may help preserve lean body mass and facilitate recovery from periods of intense physical demand. For example, dietary strategies that increase amino acid availability have been shown to stimulate protein synthesis in skeletal muscle following resistance exercise. Because military tasks also incorporate endurance exercise components, studies regarding the effects of increasing dietary amino acids following endurance exercise are warranted. The objectives of this study are to characterize the effect of endurance exercise on protein synthesis and breakdown as well as the ability of an essential amino acid supplement to influence skeletal muscle protein metabolism and its cellular and molecular regulation following endurance exercise.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P25-P50 for phase_1
Started May 2007
Typical duration for phase_1
2 active sites
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
May 1, 2007
CompletedPrimary Completion
Last participant's last visit for primary outcome
July 1, 2010
CompletedStudy Completion
Last participant's last visit for all outcomes
July 1, 2010
CompletedFirst Submitted
Initial submission to the registry
June 1, 2011
CompletedFirst Posted
Study publicly available on registry
June 6, 2011
CompletedJuly 21, 2017
June 1, 2011
3.2 years
June 1, 2011
July 19, 2017
Conditions
Keywords
Outcome Measures
Primary Outcomes (1)
Change in muscle protein synthesis after exercise or exercise with amino acid supplementation.
An average change over a 210 minute recovery period from a 60 minute endurance exercise session
Secondary Outcomes (1)
Change in intracellular signaling after exercise or exercise with amino acid supplementation.
At 30 minutes and 210 minutes after a 60 minute endurance exercise session
Study Arms (4)
Muscle protein turnover and intracellular signaling at rest
NO INTERVENTIONMuscle protein turnover and intracellular signaling are measured at rest for comparison to post-exercise muscle metabolism.
Muscle metabolism after endurance exercise
ACTIVE COMPARATORPost-exercise muscle protein metabolism was measured to determine if endurance exercise affects muscle metabolism compared to rest.
Muscle Metabolism after endurance exercise
EXPERIMENTALMuscle metabolism response to endurance exercise with essential amino acid supplementation
Muscle anabolism after endurance exercise
EXPERIMENTALMuscle anabolism after endurance exercise with essential amino acid supplementation.
Interventions
10 Gram Essential Amino Acid solutions with different leucine contents consumed during two identical endurance exercise trials
60 minute endurance exercise session
Eligibility Criteria
You may qualify if:
- Men and women aged 18 - 35 years
- Weight stable
- Recreationally active with VO2max of 40 - 50 ml/kg/min
- Refrain from taking any NSAIDS or any aspirin-containing medications
- Supervisor approval if a federal employee
You may not qualify if:
- Disease or medication that affects macronutrient metabolism and/or the ability to participate in strenuous exercise
- Allergies to foods or medications (including, but not limited to, lidocaine or phenylalanine) to be utilized in the study
- Abnormal PT/PTT test or problems with blood clotting
- Present condition of alcoholism or other substance abuse that compromises exercise capacity
- Musculoskeletal injuries that compromise the ability to exercise
- Pregnancy
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (2)
Tufts University
Boston, Massachusetts, 02111, United States
US Army Research Institute of Environmental Medicine
Natick, Massachusetts, 01760, United States
Related Publications (3)
Pasiakos SM, McClung HL, McClung JP, Margolis LM, Andersen NE, Cloutier GJ, Pikosky MA, Rood JC, Fielding RA, Young AJ. Leucine-enriched essential amino acid supplementation during moderate steady state exercise enhances postexercise muscle protein synthesis. Am J Clin Nutr. 2011 Sep;94(3):809-18. doi: 10.3945/ajcn.111.017061. Epub 2011 Jul 20.
PMID: 21775557BACKGROUNDPasiakos SM, McClung HL, McClung JP, Urso ML, Pikosky MA, Cloutier GJ, Fielding RA, Young AJ. Molecular responses to moderate endurance exercise in skeletal muscle. Int J Sport Nutr Exerc Metab. 2010 Aug;20(4):282-90. doi: 10.1123/ijsnem.20.4.282.
PMID: 20739716RESULTScofield DE, McClung HL, McClung JP, Kraemer WJ, Rarick KR, Pierce JR, Cloutier GJ, Fielding RA, Matheny RW Jr, Young AJ, Nindl BC. A novel, noninvasive transdermal fluid sampling methodology: IGF-I measurement following exercise. Am J Physiol Regul Integr Comp Physiol. 2011 Jun;300(6):R1326-32. doi: 10.1152/ajpregu.00313.2010. Epub 2011 Mar 9.
PMID: 21389329RESULT
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Stefan M Pasiakos, Ph.D.
US Army Research Institute of Environmental Medicine
Study Design
- Study Type
- interventional
- Phase
- phase 1
- Allocation
- RANDOMIZED
- Masking
- DOUBLE
- Who Masked
- PARTICIPANT, OUTCOMES ASSESSOR
- Purpose
- BASIC SCIENCE
- Intervention Model
- PARALLEL
- Sponsor Type
- FED
Study Record Dates
First Submitted
June 1, 2011
First Posted
June 6, 2011
Study Start
May 1, 2007
Primary Completion
July 1, 2010
Study Completion
July 1, 2010
Last Updated
July 21, 2017
Record last verified: 2011-06