Effect of Acute Red Raspberry Consumption on Post-prandial Oxidative Stress

NCT01242033 | Completed

• 1 other identifier: 24774
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.

Conditions

Oxidative Stress; Antioxidants

Keywords

red raspberries; post-prandial; oxidative stress; antioxidants; polyphenols; vitamin C; acute dietary intervention

Outcome Measures

Primary Outcomes (1)

• change in antioxidant capacity from baseline (-0.25 h)

  plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE)

  -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake

Secondary Outcomes (2)

• change in lipid peroxidation from baseline (-0.25 h)

  -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake

• change in thiols compared to baseline (-0.25 h)

  -025, 0.25, 0.5, 1, 2, 4 hours post-intake

Study Arms (5)

one cup red raspberries

EXPERIMENTAL

treatment meal consists of one cup red raspberries

Dietary Supplement: red raspberries

two cups red raspberries

EXPERIMENTAL

treatment meal consists of two cups red raspberries

Dietary Supplement: red raspberries

four cups red raspberries

EXPERIMENTAL

treatment meal consists of four cups red raspberries

Dietary Supplement: red raspberries

bread

PLACEBO COMPARATOR

treatment meal consists of two slices white bread

Dietary Supplement: bread controls

vitamin C

ACTIVE COMPARATOR

treatment meal consists of two slices white bread and 200 mg vitamin C in the form of supplemental ascorbic acid

Dietary Supplement: bread controls

Interventions

red raspberries DIETARY_SUPPLEMENT

single serving of one, two or four cups red raspberries

four cups red raspberries; one cup red raspberries; two cups red raspberries

bread controls DIETARY_SUPPLEMENT

two slices white bread alone or with 200 mg vitamin C supplement

bread; vitamin C

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy adults

You may not qualify if:

• blood pressure greater than 120/80 mm Hg
• body mass index greater than 24.9 kg/m\^2
• history of any chronic disease
• currently taking medications
• allergy to fruit

Sponsors & Collaborators

• University of Toronto: lead
• Unity Health Toronto: collaborator

Study Sites (1)

Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital

Toronto, Ontario, M5C 2T2, Canada

Location

Study Officials

• A V Rao, PhD

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: November 8, 2010
• First Posted: November 16, 2010
• Study Start: February 1, 2010
• Primary Completion: March 1, 2010
• Study Completion: March 1, 2010
• Last Updated: December 6, 2010

Record last verified: 2010-11

Locations

CA (1)