NCT01136395

Brief Summary

This project comprises immunological and virological analyses within a prospective clinical study of Rituximab (Rtx)-treated blood group incompatible living donor (LD) renal transplant recipients compared to blood group compatible LD recipients without Rtx induction, and of living donor compared to deceased donor renal transplant recipients treated with tacrolimus (Tacr)/mycophenolate sodium (MPS). Aim of this project is to assess short- and long-term effects of immunosuppressive therapy (Rtx induction) and of living donation on immunological and histological parameters of graft outcome and on viral replication (BK virus (BKV), JC virus (JCV), cytomegalovirus (CMV), Epstein Barr virus (EBV)) with the potential to improve long-term graft outcome and to enable risk estimation of virus disease.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
85

participants targeted

Target at P50-P75 for phase_2

Timeline
Completed

Started Jan 2010

Longer than P75 for phase_2

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2010

Completed
5 months until next milestone

First Submitted

Initial submission to the registry

June 2, 2010

Completed
1 day until next milestone

First Posted

Study publicly available on registry

June 3, 2010

Completed
9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 18, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 18, 2019

Completed
Last Updated

October 8, 2019

Status Verified

October 1, 2019

Enrollment Period

9.5 years

First QC Date

June 2, 2010

Last Update Submit

October 7, 2019

Conditions

Keywords

Kidney TransplantationRituximabLiving DonorABO Incompatible (ABOi) TransplantationImmunologyCytomegalovirus (CMV)Epstein Barr virus (EBV)Polyomavirus

Outcome Measures

Primary Outcomes (14)

  • Impact of Rtx on immune parameters predictive of graft outcome including B cell responses

    immune parameters of graft outcome: see "detailed description"

    5 years posttransplant

  • Impact of living donation on immune parameters predictive of graft outcome including B cell responses

    parameters of graft outcome: see "detailed description"

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: EBV PCR (in blood)

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: CMV PCR (in blood)

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: BKV PCR (in blood)

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: BKV PCR (in urine)

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: JCV PCR (in blood)

    5 years posttransplant

  • Impact of Rtx on virus replication

    outcome measure description: JCV PCR (in urine)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: EBV PCR (in blood)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: CMV PCR (in blood)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: BKV PCR (in blood)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: BKV PCR (in urine)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: JCV PCR (in blood)

    5 years posttransplant

  • Impact of living donation on virus replication

    outcome measure description: JCV PCR (in urine)

    5 years posttransplant

Secondary Outcomes (13)

  • Patient survival

    5 years posttransplant

  • Graft survival

    5 years posttransplant

  • Graft function and proteinuria

    5 years posttransplant

  • Graft function

    5 years posttransplant

  • Graft function

    5 years posttransplant

  • +8 more secondary outcomes

Study Arms (3)

LD kidney transplantation, ABOi

ACTIVE COMPARATOR

Living donor (LD) kidney transplantation, ABO incompatible (ABOi); Immunosuppressive treatment: Tacrolimus (Tacr)/ Mycophenolate sodium (MPS), Basiliximab induction, Rtx induction

Drug: RituximabProcedure: living donor transplantation

LD kidney transplantation, ABOc

ACTIVE COMPARATOR

Living donor (LD) kidney transplantation, ABO compatible (ABOc); Immunosuppressive treatment: Tacr/MPS, Basiliximab induction

Procedure: living donor transplantation

DD kidney transplantation

ACTIVE COMPARATOR

Deceased donor (DD) kidney transplantation, ABO compatible; Immunosuppressive treatment: Tacr/MPS, Basiliximab induction

Procedure: deceased donor transplantation

Interventions

375mg/m2 4 weeks before ABOi LD transplantation

Also known as: Blood group incompatible (ABOi) living donor (LD) renal transplantation, Anti-CD20 (cluster of differentiation 20) Monoclonal Antibody (MoAb)
LD kidney transplantation, ABOi

living donor transplantation (ABO compatible) to be compared with deceased donor transplantation (ABO compatible) in its impact on immunological parameters of graft outcome and on viral replication (CMV, EBV, BK/JC), respectively

Also known as: Blood group compatible (ABOc) LD NTx
LD kidney transplantation, ABOcLD kidney transplantation, ABOi

deceased donor transplantation (ABO compatible) to be compared with living donor transplantation (ABO compatible) in its impact on immunological parameters of graft outcome and on viral replication (CMV, EBV, BK virus (BKV), JC virus (JCV)), respectively

Also known as: Deceased donor (DD) renal transplantation
DD kidney transplantation

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • De-novo kidney transplantation
  • Deceased donors (blood group compatible) and living donors (blood group incompatible / blood group compatible)
  • First, second and third renal transplants
  • Immunized and non-immunized graft recipients
  • Age of recipients 18 years or older
  • Negative pregnancy test before transplantation

You may not qualify if:

  • Contra-indications to use Tacr and MPS, respectively
  • Contra-indications to use Rtx in the group of ABOi LD transplants
  • Chronic hepatitis B, C or HIV infection
  • Recurrent infectious disease
  • Previous hepatitis B, if no prophylactic antiviral therapy is used
  • Previous tuberculosis
  • Hemoglobin\<8,5g/dl, thrombocytes\<80.000/ul or leucocytes\<3000/ul
  • Previous vaccination with a living vaccine \<4 weeks pretransplant
  • Significant enterogastric disease such as diverticulitis (contra-indicates MPS treatment)
  • Children and adolescents (age less than 18 years)
  • Pregnancy and breast-feeding women
  • Refusal of an effective contraception in women capable of bearing children
  • Combined transplantations such as simultaneous islet/kidney transplants

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Internal Medicine, University of Giessen

Giessen, D-35392, Germany

Location

Related Publications (11)

  • Hackstein H, Renner FC, Bohnert A, Nockher A, Frommer T, Bein G, Weimer R. Dendritic cell deficiency in the blood of kidney transplant patients on long-term immunosuppression: results of a prospective matched-cohort study. Am J Transplant. 2005 Dec;5(12):2945-53. doi: 10.1111/j.1600-6143.2005.01101.x.

    PMID: 16303009BACKGROUND
  • Sadeghi M, Daniel V, Weimer R, Wiesel M, Hergesell O, Opelz G. Differential early posttransplant cytokine responses in living and cadaver donor renal allografts. Transplantation. 2003 Apr 27;75(8):1351-5. doi: 10.1097/01.TP.0000063706.52369.ED.

    PMID: 12717229BACKGROUND
  • Staak A, Renner F, Suesal C, Dietrich H, Rainer L, Kamali-Ernst S, Ernst W, Padberg W, Opelz G, Weimer R. Immunoglobulin induction therapy in renal transplant recipients: Effects on immunoglobulin and regulatory antibody levels. Transplant Proc. 2006 Dec;38(10):3483-5. doi: 10.1016/j.transproceed.2006.10.041.

    PMID: 17175311BACKGROUND
  • Weimer R, Melk A, Daniel V, Friemann S, Padberg W, Opelz G. Switch from cyclosporine A to tacrolimus in renal transplant recipients: impact on Th1, Th2, and monokine responses. Hum Immunol. 2000 Sep;61(9):884-97. doi: 10.1016/s0198-8859(00)00152-x.

    PMID: 11053632BACKGROUND
  • Weimer R, Mytilineos J, Feustel A, Preiss A, Daniel V, Grimm H, Wiesel M, Opelz G. Mycophenolate mofetil-based immunosuppression and cytokine genotypes: effects on monokine secretion and antigen presentation in long-term renal transplant recipients. Transplantation. 2003 Jun 27;75(12):2090-9. doi: 10.1097/01.TP.0000058808.37349.23.

    PMID: 12829918BACKGROUND
  • Weimer R, Staak A, Susal C, Streller S, Yildiz S, Pelzl S, Renner F, Dietrich H, Daniel V, Rainer L, Kamali-Ernst S, Ernst W, Padberg W, Opelz G. ATG induction therapy: long-term effects on Th1 but not on Th2 responses. Transpl Int. 2005 Feb;18(2):226-36. doi: 10.1111/j.1432-2277.2004.00047.x.

    PMID: 15691277BACKGROUND
  • Weimer R, Susal C, Yildiz S, Staak A, Pelzl S, Renner F, Dietrich H, Daniel V, Kamali-Ernst S, Ernst W, Padberg W, Opelz G. Post-transplant sCD30 and neopterin as predictors of chronic allograft nephropathy: impact of different immunosuppressive regimens. Am J Transplant. 2006 Aug;6(8):1865-74. doi: 10.1111/j.1600-6143.2006.01407.x. Epub 2006 Jun 9.

    PMID: 16771810BACKGROUND
  • Daniel V, Naujokat C, Sadeghi M, Renner FC, Weimer R, Opelz G. Association of high IFN-gamma plasma levels with low B-cell counts in renal transplant recipients with stable long-term graft function. Clin Transplant. 2010 Mar-Apr;24(2):281-9. doi: 10.1111/j.1399-0012.2009.01067.x. Epub 2009 Aug 27.

    PMID: 19712086BACKGROUND
  • Susal C, Dohler B, Opelz G. Graft-protective role of high pretransplantation IgA-anti-Fab autoantibodies: confirmatory evidence obtained in more than 4000 kidney transplants. The Collaborative Transplant Study. Transplantation. 2000 Apr 15;69(7):1337-40. doi: 10.1097/00007890-200004150-00021.

    PMID: 10798750BACKGROUND
  • Susal C, Pelzl S, Dohler B, Opelz G. Identification of highly responsive kidney transplant recipients using pretransplant soluble CD30. J Am Soc Nephrol. 2002 Jun;13(6):1650-6. doi: 10.1097/01.asn.0000014256.75920.5b.

    PMID: 12039995BACKGROUND
  • Weimer R, Karakizlis H, Renner F, Dietrich H, Daniel V, Susal C, Schuttler C, Kamper D, Leicht D, Worlen M, Milchsack K, Renner L, Stich M, Grone HJ, Hecker A, Horbelt R, Padberg W, Opelz G. Long-term compromised immune regulation after rituximab induction in blood group incompatible (ABOi) living-donor renal transplantation - 5 year results of a prospective pilot study. Front Immunol. 2025 Dec 12;16:1706158. doi: 10.3389/fimmu.2025.1706158. eCollection 2025.

MeSH Terms

Conditions

Virus DiseasesEpstein-Barr Virus Infections

Interventions

RituximabKidney TransplantationAntibodies, Monoclonal

Condition Hierarchy (Ancestors)

InfectionsHerpesviridae InfectionsDNA Virus InfectionsTumor Virus Infections

Intervention Hierarchy (Ancestors)

Antibodies, Monoclonal, Murine-DerivedAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsSerum GlobulinsGlobulinsRenal Replacement TherapyTherapeuticsOrgan TransplantationTransplantationSurgical Procedures, OperativeUrologic Surgical ProceduresUrogenital Surgical Procedures

Study Officials

  • Rolf Weimer, Prof. Dr.

    University of Giessen, Department of Internal Medicine

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
head of renal transplant program, University of Giessen

Study Record Dates

First Submitted

June 2, 2010

First Posted

June 3, 2010

Study Start

January 1, 2010

Primary Completion

June 18, 2019

Study Completion

June 18, 2019

Last Updated

October 8, 2019

Record last verified: 2019-10

Data Sharing

IPD Sharing
Will share

all individual participant data are available to the members of the study group who treat the patients in the Department of Internal Medicine, University Clinic of Giessen (i.e. all participants of the study group working in the Department of Internal Medicine)

Shared Documents
STUDY PROTOCOL, SAP, ICF, CSR, ANALYTIC CODE
Time Frame
data will be available up to 1year post study completion
Access Criteria
seen above

Locations