Study on the Cardioprotection and Humoral Mechanism of Limb Ischemia Preconditioning

NCT01118000 | Completed DMC

• 1 other identifier: GDNSFC7001664
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Numerous studies Have shown that limb ischemic preconditioning can protect vital organs (including the heart) from ischemia-reperfusion injury,which has a broad application prospect.But its mechanism is still unclear. Evidence showed that humoral mechanisms may play an important role. This study was carried out on the limb ischemic preconditioning in healthy volunteers, collected their serum at different time points before and after treatment,classified and identified the serum proteins during different periods of limb ischemic preconditioning,by using methods including high abundant protein removal,Two-dimensional electrophoresis chromatography and mass spectrometry,then analysed activation and synthesis of the proteins in order to search for the proteins or peptides whose synthesis was activated by ischemic preconditioning. This study will be the first systemic explore of the changes in serum proteins of limb ischemic preconditioning in human body,and lay theoretical basis for the clinical application of limb ischemic preconditioning and for further explore of its humoral mechanism,thus provide clues for searching for protein or peptide having protective effects for organs.

Conditions

Ischemic Preconditioning

Keywords

ischemic preconditioning,serum proteins,human

Outcome Measures

Primary Outcomes (1)

• Transient limb ischemia would change expression of several serum proteins in humans,indicating a humoral mechanism for its organ protection.

  Proteomics analysis of the serum proteins.

  two years

Secondary Outcomes (1)

• A questionnaire study of the acceptability of transient limb ischemia in healthy conscious subjects.

  one year

Study Arms (1)

limb ischemia preconditioning

EXPERIMENTAL

limb ischemic preconditioning consists of three 5-min cycles of left upper limb ischemia induced by a blood pressure cuff placed on the left upper arm and inflated to 200 mmHg,with an intervention 5-min of reperfusion during which the cuff was deflated.

Procedure: limb ischemia preconditioning

Interventions

limb ischemia preconditioning PROCEDURE

limb ischemic preconditioning consists of three 5-min cycles of left upper limb ischemia induced by a blood pressure cuff placed on the left upper arm and inflated to 200 mmHg,with an intervention 5-min of reperfusion during which the cuff was deflated.

Also known as: remote ischemia preconditioning

limb ischemia preconditioning

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy volunteers aging 20-30 years old
• no cardiac，hepatic or renal，peripheral vascular diseases
• be willing to enter our study and sign an written informed consent

You may not qualify if:

• younger than 20 years old or older than 30 years old
• having cardiac，hepatic or renal，peripheral vascular diseases
• refuse to enter our study or sign an written informed consent

Sponsors & Collaborators

• Sun Yat-sen University: lead

Study Sites (1)

proteomics lab of Sun Yat-sen University

Guangzhou, Guangdong, 510080, China

Location

Related Links

• PUMED
• Effect of remote ischaemic preconditioning on myocardial injury in patients undergoing coronary artery bypass graft surgery: a randomised controlled trial.
• Ischemic preconditioning prevents endothelial injury and systemic neutrophil activation during ischemia-reperfusion in humans

Study Officials

• Jin S qing, Doctor

  Sixth Affiliated Hospital, Sun Yat-sen University

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: April 26, 2010
• First Posted: May 6, 2010
• Study Start: September 1, 2009
• Primary Completion: November 1, 2009
• Study Completion: March 1, 2010
• Last Updated: July 28, 2010

Record last verified: 2010-04

Locations

CN (1)