Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome

NCT01028898 | Unknown DMC

• 1 other identifier: IBS and metabolism
• Study Type: observational
• Target: 180
• Locations: 0 countries
• Sites: N/A

Brief Summary

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Conditions

Irritable Bowel Syndrome; Digestive System Diseases; Gastrointestinal Diseases; Intestinal Diseases; Colonic Diseases; Colonic Diseases, Functional

Keywords

IBS; proteomics; fluorescent quantitation polymerase chain reaction; Western blot; immunohistochemistry staining (IHC); ATP content; micRNA

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

IBS patients (IBS-D and IBS-C)and healthy control

You may qualify if:

• Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

You may not qualify if:

• Patients with organic illnesses or a history of major abdominal surgery
• Major psychotic episode, mental retardation or dementia
• Pregnant women and lactational women
• Postinfectious IBS, PI-IBS

Sponsors & Collaborators

• Chinese PLA General Hospital: lead

Related Publications (1)

• Wang WF, Li X, Guo MZ, Chen JD, Yang YS, Peng LH, Wang YH, Zhang CY, Li HH. Mitochondrial ATP 6 and 8 polymorphisms in irritable bowel syndrome with diarrhea. World J Gastroenterol. 2013 Jun 28;19(24):3847-53. doi: 10.3748/wjg.v19.i24.3847.

  PMID: 23840124 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

The investigators enrolled IBS-D and IBS-C patients on the Rome III criteria and healthy volunteers as normal control group. Biopsies were taken from the cecum and sigmoid colon under direct vision. Five pieces were taken separately at each site. Four pieces were processed by cold saline water and frozen in liquid nitrogen immediately while the other one was fixed in formalin and then embedded in paraffin three days later.

MeSH Terms

Conditions

Irritable Bowel Syndrome; Digestive System Diseases; Gastrointestinal Diseases; Intestinal Diseases; Colonic Diseases; Colonic Diseases, Functional

Study Officials

• YANG Yun-sheng, MD

  Chinese PLA General Hospital

  STUDY CHAIR

• ZHANG Chun-yan, doctor

  Chinese PLA General Hospital

  PRINCIPAL INVESTIGATOR

• WANG Weifeng, doctor

  Chinese PLA General Hospital

  PRINCIPAL INVESTIGATOR

• YAO XIN, DOCTOR

  Chinese PLA General Hospital

  PRINCIPAL INVESTIGATOR

• PENG LIHUA, DOCTOR

  Chinese PLA General Hospital

  PRINCIPAL INVESTIGATOR

• YU YUANZI, DOCTOR

  Chinese PLA General Hospital

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: December 8, 2009
• First Posted: December 9, 2009
• Study Start: January 1, 2006
• Primary Completion: August 1, 2011
• Study Completion: December 1, 2012
• Last Updated: February 11, 2011

Record last verified: 2006-12