TRANSCRIBE (Transcriptomic Analysis of Left Ventricular Gene Expression)

NCT00985049 | Recruiting

• 2 other identifiers: 2007p002323R01HL118266
• Study Type: observational
• Target: 350
• Locations: 1 country
• Sites: 1

Brief Summary

Hypothesis: The investigators hypothesize that genetic variants will alter gene expression in response to ischemia during cardiopulmonary bypass. To test this, the investigators will be taking small pieces of heart tissue from patients undergoing heart surgery before and after this period of ischemia. This will be compared to blood gene expression in blood cells and analyzed with the patients' genetic profile. This might allow us to assess the amount of inflammation that occurs when patients are on the heart-lung machine as well as examine the effects genes have on this critical time period.

Conditions

Cardiovascular Diseases

Outcome Measures

Primary Outcomes (1)

• Differential gene expression

  Immediate

Eligibility Criteria

• Age: 20 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Adult patients undergoing cardiac surgery

You may qualify if:

• Aged greater than 20 years
• Undergoing aortic valve surgery with or without CABG surgery
• Receive and apical vent as part of the surgery

You may not qualify if:

• Will not receive an apical left ventricular vent

Sponsors & Collaborators

• Brigham and Women's Hospital: lead
• American Heart Association: collaborator
• Harvard Medical School (HMS and HSDM): collaborator
• National Heart, Lung, and Blood Institute (NHLBI): collaborator

Study Sites (1)

Brigham and Women's Hospital

Boston, Massachusetts, 02115, United States

RECRUITING

Related Publications (5)

• Heydarpour M, Ejiofor J, Gilfeather M, Stone G, Gorham J, Seidman CE, Seidman JG, Yammine M, Body SC, Aranki SF, Muehlschlegel JD. Molecular Genetics of Lidocaine-Containing Cardioplegia in the Human Heart During Cardiac Surgery. Ann Thorac Surg. 2018 Nov;106(5):1379-1387. doi: 10.1016/j.athoracsur.2018.06.016. Epub 2018 Jul 17.

  PMID: 30028983 RESULT

• Saddic LA, Nicoloro SM, Gupta OT, Czech MP, Gorham J, Shernan SK, Seidman CE, Seidman JG, Aranki SF, Body SC, Fitzgibbons TP, Muehlschlegel JD. Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia. Cardiovasc Diabetol. 2017 Jul 7;16(1):87. doi: 10.1186/s12933-017-0567-x.

  PMID: 28687077 RESULT

• Saddic LA, Sigurdsson MI, Chang TW, Mazaika E, Heydarpour M, Shernan SK, Seidman CE, Seidman JG, Aranki SF, Body SC, Muehlschlegel JD. The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle. Circ Cardiovasc Genet. 2017 Jan;10(1):e001534. doi: 10.1161/CIRCGENETICS.116.001534.

  PMID: 28115490 RESULT

• Sigurdsson MI, Saddic L, Heydarpour M, Chang TW, Shekar P, Aranki S, Couper GS, Shernan SK, Seidman JG, Body SC, Muehlschlegel JD. Allele-specific expression in the human heart and its application to postoperative atrial fibrillation and myocardial ischemia. Genome Med. 2016 Dec 6;8(1):127. doi: 10.1186/s13073-016-0381-1.

  PMID: 27923400 RESULT

• Saddic LA, Chang TW, Sigurdsson MI, Heydarpour M, Raby BA, Shernan SK, Aranki SF, Body SC, Muehlschlegel JD. Integrated microRNA and mRNA responses to acute human left ventricular ischemia. Physiol Genomics. 2015 Oct;47(10):455-62. doi: 10.1152/physiolgenomics.00049.2015. Epub 2015 Jul 14.

  PMID: 26175501 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

DNA, EDTA , peripheral blood leucocytes, and left ventricle myocardium are all stored in -80C frreezer at BWH.

MeSH Terms

Conditions

Cardiovascular Diseases

Study Officials

• Jochen D. Muehlschlegel, MD, MMSc, MBA

  Johns Hopkins University

  PRINCIPAL INVESTIGATOR

• Sergey Karamnov, MD

  Brigham and Women's Hospital

  STUDY DIRECTOR

Central Study Contacts

Jochen D Muehlschlegel, MD, MMSc, MBA

CONTACT

4109558408; dmuehlsch@jhu.edu

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor of Anesthesia

Study Record Dates

• First Submitted: September 24, 2009
• First Posted: September 28, 2009
• Study Start: March 1, 2009
• Primary Completion: December 1, 2025
• Study Completion: December 1, 2025
• Last Updated: August 22, 2024

Record last verified: 2024-08

Locations

US (1)