NCT00960752

Brief Summary

The goal of this clinical research study is to learn if the vaccines, gp100(g209-2M), and MAGE-3, when given in combination with resiquimod (R848), can help to stimulate the immune system against melanoma.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
47

participants targeted

Target at P25-P50 for phase_2

Timeline
Completed

Started May 2010

Longer than P75 for phase_2

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 17, 2009

Completed
1 day until next milestone

First Posted

Study publicly available on registry

August 18, 2009

Completed
9 months until next milestone

Study Start

First participant enrolled

May 20, 2010

Completed
10.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 20, 2020

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

July 20, 2020

Completed
1.1 years until next milestone

Results Posted

Study results publicly available

August 13, 2021

Completed
Last Updated

August 13, 2021

Status Verified

July 1, 2021

Enrollment Period

10.2 years

First QC Date

August 17, 2009

Results QC Date

July 20, 2021

Last Update Submit

July 20, 2021

Conditions

Keywords

gp100 peptideMAGE-3 peptideR848ResiquimodToll Like ReceptorTLRT-cellsTumorMetastaticLesionsInjection Site

Outcome Measures

Primary Outcomes (1)

  • Comparison of Immune Responses of Vaccine+R848 to Vaccine Alone

    T-cell response to gp100(g209-2M) +/- MAGE3 measured at 8 weeks using a tetramer/multimer assay measured by flow cytometry. Based on the number of gp100(g209-2M) +/- MAGE3 T-cells measured, categorized as either immune responders or immune non-responders. T-cell response to the gp100(g209-2M) + /- MAGE3 peptide in each participant defined as ≥0.1% gp100(g209-2M) tetramer+ cells in the CD8+ T-cell population or ≥0.1% MAGE3 multimer cells in the CD4+ T-cell population.

    8 weeks

Secondary Outcomes (1)

  • Composite of Immune Cell Infiltration for pDCs, mDCs, and NK Cells in Tumor Biopsy Samples

    8 weeks

Study Arms (3)

Group 1: gp100 and MAGE-3 + R848

ACTIVE COMPARATOR

1 ml of gp100 peptide vaccine and 1 ml of MAGE-3 peptide vaccine daily for 8 weeks with R848 Gel applied to gp100 injection site immediately. Injections given intradermally and subcutaneously at 2 separate sites in separate extremities. Half of each dose given one way, and half given the other way. Vaccines given on the same day. Participant receives a total of 4 injections each week. After 8 weeks, if participant has melanoma lesions, R848 applied to half of the lesions on body for 16 weeks.

Drug: gp100Drug: R848 gelDrug: MAGE-3

Group 2: gp100 and MAGE-3

ACTIVE COMPARATOR

1 ml of gp100 peptide vaccine and 1 ml of MAGE-3 peptide vaccine daily for 8 weeks. Injections given intradermally and subcutaneously at 2 separate sites in separate extremities. Half of each dose given one way, and half given the other way. Vaccines given on the same day. Participant receives a total of 4 injections each week. After 8 weeks, if participant has melanoma lesions, R848 applied to half of the lesions on body for 16 weeks.

Drug: gp100Drug: MAGE-3

Group 3-Metastatic Melanoma: gp100 + MAGE-3 + R848

ACTIVE COMPARATOR

1 ml of gp100 peptide vaccine and 1 ml of MAGE-3 peptide vaccine daily for 8 weeks with R848 Gel applied to gp100 injection site immediately. Injections given intradermally and subcutaneously at 2 separate sites in separate extremities. Half of each dose given one way, and half given the other way. Vaccines given on the same day. Participant receives a total of 4 injections each week. After 8 weeks, if participant has melanoma lesions, R848 applied to half of the lesions on body for 16 weeks.

Drug: gp100Drug: R848 gelDrug: MAGE-3

Interventions

gp100DRUG

1 ml of gp100 peptide vaccine: 0.5 ml administered intradermally and the other 0.5 ml subcutaneously in the same extremity weekly (every 7 days +/- 2 days) for 8 weeks.

Also known as: gp100 peptide, g209-2M
Group 1: gp100 and MAGE-3 + R848Group 2: gp100 and MAGE-3Group 3-Metastatic Melanoma: gp100 + MAGE-3 + R848

Applied to the gp100 vaccine injection site immediately following the injection, allowed to air dry for 30 minutes then covered with gauze dressing.

Also known as: Resiquimod
Group 1: gp100 and MAGE-3 + R848Group 3-Metastatic Melanoma: gp100 + MAGE-3 + R848
MAGE-3DRUG

1 ml of MAGE-3 peptide vaccine: 0.5 ml administered intradermally and the other 0.5 ml subcutaneously in the same extremity weekly (every 7 days +/- 2 days) for 8 weeks.

Also known as: MAGE-3 peptide
Group 1: gp100 and MAGE-3 + R848Group 2: gp100 and MAGE-3Group 3-Metastatic Melanoma: gp100 + MAGE-3 + R848

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • HLA-A\*0201 positive (to enable immunization with the HLA class I restricted gp100(g209-2M) peptide). Stage IIB or IIC patients will be enrolled after review and approval by the PI. (a tool to determine the projected survival at 5 years, like, but not limited to, the nomogram at www.melanomaprognosis.org. If the projected survival is less than 50% at 5 years, then the patient is considered for enrollment. This is with the recognition that the adjuvant, if effective offers a significant impact in that group of stage II patients.)
  • Patients \>/= 18 years old with histologically documented metastatic melanoma with a. (Metastatic disease cohort) Measurable disease, stage IIIB, IIIC (in transit lesions with or without nodal metastases) that includes lesions accessible for biopsies or IV M1B b. (Adjuvant cohort) subjects who are NED and stage III or IV. This includes patients with stage IV disease resected to NED. Stage IIB or IIC patients will be enrolled after review and approval by the PI.
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0-1
  • At least 2 biopsiable easily accessible cutaneous and subcutaneous lesions in patients in the metastatic disease cohort
  • White Blood Count (WBC) \>/= 3000/mm\^3 (part 1 \& 2)
  • Platelet count \>/= 90,000/mm\^3 (part 1 \& 2)
  • Serum alanine aminotransferase (ALT) and Aspartate Aminotransferase (AST) \</= 3 X upper limit of normal (ULN) (part 1 \& 2)
  • Total bilirubin \</= 2 X ULN, except for patients with Gilbert's syndrome who must have a total bilirubin less than 3.0 mg/dl Total bilirubin \</= 2 X ULN, except for patients with Gilbert's syndrome who must have a total bilirubin less than 3.0 mg/dl (part 1 \& 2)
  • Seronegative for human immunodeficiency virus (HIV) antibody. (The experimental treatment being evaluated in this protocol depends on an intact immune system. Patients who are HIV seropositive can have decreased immune competence and thus may be less responsive to the experimental treatment and more susceptible to its toxicities.)
  • Negative pregnancy test for women of childbearing potential (WOCBP) A WOCBP has not undergone a hysterectomy or who has not been naturally postmenopausal for at least 12 consecutive months (i.e., who has had menses at any time in the preceding 12 consecutive months)
  • Patients of both gender must practice a barrier method of birth control while participating in this trial.

You may not qualify if:

  • Active autoimmune disease requiring active therapy with any form of steroid or immunosuppressive therapy or a documented history of any of the following: inflammatory bowel disease; regional enteritis; systemic lupus erythematosis; Sjogren's syndrome; inflammatory neurologic disorder such as multiple sclerosis; or any immune mediated disease that can cause life-threatening symptoms or severe organ/tissue damage in the opinion of the principle investigator.
  • Concurrent systemic or inhaled steroid therapy
  • Any form of active primary or secondary immunodeficiency
  • Prior malignancy except for the following: adequately treated basal cell or squamous cell skin cancer, in-situ cervical cancer, thyroid cancer (except anaplastic) or any cancer from which the patient has been disease-free for 2 years
  • History of immunization with gp100(g209-2M)
  • Active systemic infections requiring intravenous antibiotics
  • Women who are breastfeeding
  • Prior systemic therapy, radiation therapy or intracavitary surgery (intra-thoracic, intra-abdominal or intracranial) within 28 days of starting study treatment.
  • Patients on chronic anticoagulation such as Aspirin, Plavix, or Coumadin who cannot have anticoagulation held for procedures are not eligible due to the need for leukapheresis

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Texas MD Anderson Cancer Center

Houston, Texas, 77030, United States

Location

Related Links

MeSH Terms

Conditions

MelanomaNeoplasmsNeoplasm Metastasis

Interventions

gp100 Melanoma Antigeng209-2M melanoma peptideresiquimodMAGEA3 protein, human

Condition Hierarchy (Ancestors)

Neuroendocrine TumorsNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms by Histologic TypeNeoplasms, Nerve TissueNevi and MelanomasSkin NeoplasmsNeoplasms by SiteSkin DiseasesSkin and Connective Tissue DiseasesNeoplastic ProcessesPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Membrane ProteinsProteinsAmino Acids, Peptides, and ProteinsMelanoma-Specific AntigensNeoplasm ProteinsAntigens, NeoplasmAntigensBiological Factors

Results Point of Contact

Title
Michael Davis, PHD., Chair, Melanoma Medical Oncology
Organization
UT MD Anderson Cancer Center

Study Officials

  • Michael A. Davies, PHD

    M.D. Anderson Cancer Center

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
FACTORIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 17, 2009

First Posted

August 18, 2009

Study Start

May 20, 2010

Primary Completion

July 20, 2020

Study Completion

July 20, 2020

Last Updated

August 13, 2021

Results First Posted

August 13, 2021

Record last verified: 2021-07

Locations