NCT00867854

Brief Summary

This laboratory-based sub-study of ATN 061 and ATN 071 will examine the effect of early treatment followed by treatment de-intensification to atazanavir/ritonavir (ATV/r) monotherapy on steady-state frequencies of replication-competent CD4+ T cell Human Immunodeficiency Virus (HIV)-1 reservoirs or cell-associated infectivity (CAI) and persistent low-level viremia (LLV), and their contribution to successful long-term control of HIV-1 replication among HIV-1 infected adolescents and young adults.

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
34

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Feb 2009

Typical duration for all trials

Geographic Reach
2 countries

17 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

February 1, 2009

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

March 22, 2009

Completed
2 days until next milestone

First Posted

Study publicly available on registry

March 24, 2009

Completed
2.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

October 1, 2011

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

October 1, 2011

Completed
Last Updated

February 28, 2017

Status Verified

March 1, 2016

Enrollment Period

2.7 years

First QC Date

March 22, 2009

Last Update Submit

February 27, 2017

Conditions

Keywords

de-intensificationHAARTmonotherapypersistent low-level viremia (LLV)treatment experienced

Outcome Measures

Primary Outcomes (4)

  • Steady-state frequencies of replication-competent CD4+ T cell HIV-1 reservoirs in participants starting HAART before DHHS guidelines (CD4+ T cell levels < 350 cells/mm3) vs. those initiating HAART by current DHHS guidelines.

    80 weeks

  • Quantitative changes in LLV between 6.5 and 50 copies/ml in participants starting early therapy & de-intensifying to ATV/r monotherapy vs. those initiating HAART at CD4+ T cell levels < 350 cells/mm3 & maintaining standard HAART.

    80 weeks

  • Quantitative changes in viral diversity during HAART in participants initiating early therapy & de-intensifying to ATV/r monotherapy vs those initiating HAART at CD4+ T cell levels < 350 cells/mm3 & maintaining standard HAART.

    80 weeks

  • Effect of viral diversity in replication-competent CD4+ T cell reservoirs & low viremia variants before de-intensification on successful control of HIV-1 replication during ATV/r maintenance in participants starting HAART before DHHS guidelines.

    80 weeks

Secondary Outcomes (1)

  • To examine the contribution of LLV genotypes, through analysis of the Gag/protease and RT, among subjects who developed rebound viremia above 50 copies/ml during treatment de-intensification to ATV/r.

    80 weeks

Study Arms (2)

Experimental

25 evaluable subjects from the experimental arm of ATN 061 who undergo de-intensification to boosted atazanavir (ATV) with VL suppression of \< 100 copies/ml and CD4+ T cells \> 350 cells/mm3 at week 48 and maintain VL suppression to \< 400 copies/ml with stable CD4+ T cell counts after week 48.

Other: Blood draw

Control

25 evaluable subjects from ATN 071 will also be enrolled. These subjects will have initiated HAART according to current DHHS guidelines (CD4+ T cells \< 350 cells/mm3), had viral load suppression to \< 100 copies/ml at 24 through 48 weeks on HAART and maintained suppression to \< 400 copies/ml through week 80.

Other: Blood draw

Interventions

This sub-study does not involve additional treatment of any ATN 061 or ATN 071 study subjects. The only intervention involved is the requirement for whole blood collection (40 ml and 60 ml) to be drawn at the same time as four ATN 061 study visits (36, 48, 56, and 80 weeks) for subjects co-enrolled into ATN 061. When these time points coincide with ATN 061 Central Laboratory samples, the 60 ml blood sample will not be collected. For subjects co-enrolled into ATN 071, there are also two samples of whole blood collection (40 ml and 60 ml) required to be drawn at four time points but at weeks 36, 48, 56, and 80 after the initiation of HAART.

ControlExperimental

Eligibility Criteria

Age18 Years - 24 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)
Sampling MethodNon-Probability Sample
Study Population

Twenty-five subjects enrolled in Arm A of ATN 061 who started highly-active antiretroviral therapy (HAART) at CD4+ T cells \> 350 cells/cubic millimeter (mm3) and are undergoing treatment de-intensification at week 48 of HAART. Twenty-five "control" subjects initiating HAART based on current Department of Health and Human Services (DHHS) guidelines at CD4+ T cell levels \< 350 cells/mm3 and maintained on standard HAART.

You may qualify if:

  • Participants
  • Currently on treatment with an ATV/r-based HAART regimen (ATV/r, FTC, TDF is the preferred regimen);
  • HIV-1 viral load \< 100 copies at week 24;
  • CD4+ T cell count \> 350 cells/mm3 at week 24; and
  • Able to provide informed consent for the sub-study and adhere to the protocol.
  • Participants
  • Initiated HAART according to current DHHS guidelines (CD4+ T cells \< 350 cells/ mm3);
  • Currently on treatment with a PI-containing HAART regimen; subjects taking a protease inhibitor OTHER than ATV/r must receive approval by the team via the ATN QNS;
  • Plasma HIV-1 viral load \< 100 copies at week 24 on HAART; measurement to be collected from clinical care results contained in the medical record at the clinical site within +/- 30 days of week 24 on therapy;
  • CD4+ T cell count \> 350 cells/mm3 at week 24 on HAART; measurement to be collected from clinical care results contained in the medical record at the clinical site within +/- 30 days of week 24 on therapy; and
  • Able to provide informed consent for the sub-study and adhere to the protocol.

You may not qualify if:

  • Currently enrolled in the Standard Care Arm of ATN 061;
  • Pregnancy or breast feeding;
  • Severe (Grade ≥ 3) anemia or other conditions that would not allow adequate blood volume to be drawn;
  • Active treatment for systemic infections;
  • Treatment with immune modulators, including immunosuppressive or immune modulating therapy (IL-2, intravenous gammaglobulin, and therapeutic or other experimental vaccines including HIV-1 vaccine given for primary prevention at any time (short courses (\<14 days) of prednisone for reactive airway disease (RAD) are permitted);
  • Active hepatitis B infection as defined by Hepatitis B antigen (Ag) positive;
  • Disallowed Medications (see Section 5.3.2);
  • Active drug or alcohol use or dependence that, in the opinion of the site personnel, would interfere with adherence to the study; or
  • History of chronic renal insufficiency or Grade 3 or greater serum creatinine.
  • History of an Acquired Immunodeficiency Syndrome (AIDS)-defining illness;
  • Meets any ATN 061 premature study discontinuation criteria.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (17)

Children's Hospital of Los Angeles

Los Angeles, California, 90027, United States

Location

University of California at San Francisco

San Francisco, California, 94118, United States

Location

Children's National Medical Center

Washington D.C., District of Columbia, 20010, United States

Location

Howard University - IMPAACT Site

Washington D.C., District of Columbia, 20060, United States

Location

Children's Diagnostic and Teatment Center

Fort Lauderdale, Florida, 33316, United States

Location

University of Miami School of Medicine

Miami, Florida, 33101, United States

Location

University of South Florida College of Medicine

Tampa, Florida, 33606, United States

Location

John Stroger Jr. Hospital of Cook County

Chicago, Illinois, 60612, United States

Location

Children's Memorial Hospital

Chicago, Illinois, 60614, United States

Location

Tulane Medical Center

New Orleans, Louisiana, 70112, United States

Location

University of Maryland

Baltimore, Maryland, 21201, United States

Location

Johns Hopkins University - IMPAACT Site

Baltimore, Maryland, 21287, United States

Location

Mount Sinai Medical Center

New York, New York, 10128, United States

Location

Montefiore Medical Center

The Bronx, New York, 10467, United States

Location

Children's Hospital of Philadelphia

Philadelphia, Pennsylvania, 19104, United States

Location

St. Jude Children's Research Hospital

Memphis, Tennessee, 38105, United States

Location

University of Puerto Rico

San Juan, 00927, Puerto Rico

Location

Related Links

Biospecimen

Retention: SAMPLES WITH DNA

Whole blood

MeSH Terms

Conditions

HIV Infections

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Blood-Borne InfectionsCommunicable DiseasesInfectionsSexually Transmitted Diseases, ViralSexually Transmitted DiseasesLentivirus InfectionsRetroviridae InfectionsRNA Virus InfectionsVirus DiseasesGenital DiseasesUrogenital DiseasesImmunologic Deficiency SyndromesImmune System Diseases

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • Deborah Persaud, M.D.

    Adolescent Trials Network

    STUDY CHAIR

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 22, 2009

First Posted

March 24, 2009

Study Start

February 1, 2009

Primary Completion

October 1, 2011

Study Completion

October 1, 2011

Last Updated

February 28, 2017

Record last verified: 2016-03

Locations