NCT00760383

Brief Summary

The general hypothesis is that in older adults muscle regrowth after an acute musculoskeletal stress will be positively influenced by traditional physical rehabilitation, and further enhanced by nutritional supplementation. Using state-of-the-art stable isotope methodologies for the study of muscle metabolism and methodologies for the measurement of cell signaling, we will test the following specific hypotheses: 1) Total knee arthroplasty (TKA) induces an acute net protein catabolism mainly by reducing muscle protein synthesis; 2) TKA induced catabolism is attenuated by the ingestion of essential amino acids (EAA); 3) EAA supplementation in combination with physical therapy (PT) will stimulate muscle protein synthesis and mTOR signaling to a greater extent than PT with Placebo; and 4) EAA supplementation during TKA PT rehabilitation will improve muscle strength, muscle volume and functional outcomes to a greater extent than PT with Placebo. Public Benefit: This research will focus rehabilitation efforts on specific and currently unresolved mechanisms responsible for muscle loss following total knee replacement in older adults. While knee pain due to bone arthritis is often alleviated after knee replacement, complete return of physical function and independence is difficult to achieve. This research will help to restore physical function and independence in the rapidly growing population of older adults with knee arthritis.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jun 2008

Longer than P75 for not_applicable

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2008

Completed
4 months until next milestone

First Submitted

Initial submission to the registry

September 25, 2008

Completed
1 day until next milestone

First Posted

Study publicly available on registry

September 26, 2008

Completed
5.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2013

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2013

Completed
1 year until next milestone

Results Posted

Study results publicly available

December 4, 2014

Completed
Last Updated

December 4, 2014

Status Verified

December 1, 2014

Enrollment Period

5.5 years

First QC Date

September 25, 2008

Results QC Date

April 17, 2014

Last Update Submit

December 1, 2014

Conditions

Keywords

Total Knee ArthroplastyOsteoarthritisMuscle MetabolismRehabilitationPhysical Therapy

Outcome Measures

Primary Outcomes (2)

  • Stair Time up

    6 weeks

  • Quadriceps Muscle Strength

    6 weeks

Secondary Outcomes (1)

  • Mid-thigh Muscle Volume

    6 weeks

Study Arms (2)

EAA+PT

EXPERIMENTAL

20 g EAA daily for 7 days prior to TKA surgery and for 14 days after surgery.

Dietary Supplement: Essential amino acids

ALA+PT

PLACEBO COMPARATOR

20 g NEAA daily for 7 days prior to TKA surgery and for 14 days after surgery.

Dietary Supplement: Alanine

Interventions

Essential amino acidsDIETARY_SUPPLEMENT

Subjects will ingest 20 grams of essential amino acids (EAA) daily for 7 days prior to total knee arthroplasty (TKA) surgery and for 14 days after surgery daily. On the days they are seen by physical therapy (PT) they will ingest the EAA supplement 30 minutes after the end of each PT rehabilitation session.

Also known as: Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Valine
EAA+PT
AlanineDIETARY_SUPPLEMENT

Subjects will ingest 20 grams of non-essential amino acid (NEAA) daily for 7 days prior to total knee arthroplasty (TKA) surgery and for 14 days after surgery daily. On the days they are seen by physical therapy (PT) they will ingest the NEAA supplement 30 minutes after the end of each PT rehabilitation session.

ALA+PT

Eligibility Criteria

Age60 Years - 80 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Total Knee Arthroplasty surgical candidate

You may not qualify if:

  • Overt muscle disease

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

University of Oregon

Eugene, Oregon, 97401-1240, United States

Location

Slocum Center for Orthopedics and Sports Medicine

Eugene, Oregon, 97401, United States

Location

Related Publications (9)

  • Dreyer HC, Drummond MJ, Pennings B, Fujita S, Glynn EL, Chinkes DL, Dhanani S, Volpi E, Rasmussen BB. Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am J Physiol Endocrinol Metab. 2008 Feb;294(2):E392-400. doi: 10.1152/ajpendo.00582.2007. Epub 2007 Dec 4.

    PMID: 18056791BACKGROUND
  • Fujita S, Dreyer HC, Drummond MJ, Glynn EL, Cadenas JG, Yoshizawa F, Volpi E, Rasmussen BB. Nutrient signalling in the regulation of human muscle protein synthesis. J Physiol. 2007 Jul 15;582(Pt 2):813-23. doi: 10.1113/jphysiol.2007.134593. Epub 2007 May 3.

    PMID: 17478528BACKGROUND
  • Dreyer HC, Fujita S, Cadenas JG, Chinkes DL, Volpi E, Rasmussen BB. Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle. J Physiol. 2006 Oct 15;576(Pt 2):613-24. doi: 10.1113/jphysiol.2006.113175. Epub 2006 Jul 27.

    PMID: 16873412BACKGROUND
  • Fujita S, Dreyer HC, Drummond MJ, Glynn EL, Volpi E, Rasmussen BB. Essential amino acid and carbohydrate ingestion before resistance exercise does not enhance postexercise muscle protein synthesis. J Appl Physiol (1985). 2009 May;106(5):1730-9. doi: 10.1152/japplphysiol.90395.2008. Epub 2008 Jun 5.

    PMID: 18535123BACKGROUND
  • Drummond MJ, Dreyer HC, Pennings B, Fry CS, Dhanani S, Dillon EL, Sheffield-Moore M, Volpi E, Rasmussen BB. Skeletal muscle protein anabolic response to resistance exercise and essential amino acids is delayed with aging. J Appl Physiol (1985). 2008 May;104(5):1452-61. doi: 10.1152/japplphysiol.00021.2008. Epub 2008 Mar 6.

    PMID: 18323467BACKGROUND
  • Dreyer HC, Blanco CE, Sattler FR, Schroeder ET, Wiswell RA. Satellite cell numbers in young and older men 24 hours after eccentric exercise. Muscle Nerve. 2006 Feb;33(2):242-53. doi: 10.1002/mus.20461.

    PMID: 16315322BACKGROUND
  • Dreyer HC, Volpi E. Role of protein and amino acids in the pathophysiology and treatment of sarcopenia. J Am Coll Nutr. 2005 Apr;24(2):140S-145S. doi: 10.1080/07315724.2005.10719455.

    PMID: 15798081BACKGROUND
  • Ratchford SM, Bailey AN, Senesac HA, Hocker AD, Smolkowski K, Lantz BA, Jewett BA, Gilbert JS, Dreyer HC. Proteins regulating cap-dependent translation are downregulated during total knee arthroplasty. Am J Physiol Regul Integr Comp Physiol. 2012 Mar 15;302(6):R702-11. doi: 10.1152/ajpregu.00601.2011. Epub 2011 Dec 28.

  • Dreyer HC, Strycker LA, Senesac HA, Hocker AD, Smolkowski K, Shah SN, Jewett BA. Essential amino acid supplementation in patients following total knee arthroplasty. J Clin Invest. 2013 Nov;123(11):4654-66. doi: 10.1172/JCI70160. Epub 2013 Oct 25.

Related Links

MeSH Terms

Conditions

Osteoarthritis

Interventions

Amino Acids, EssentialHistidineIsoleucineLeucineLysineMethioninePhenylalanineThreonineValineAlanine

Condition Hierarchy (Ancestors)

ArthritisJoint DiseasesMusculoskeletal DiseasesRheumatic Diseases

Intervention Hierarchy (Ancestors)

Amino AcidsAmino Acids, Peptides, and ProteinsAmino Acids, CyclicAmino Acids, Branched-ChainAmino Acids, BasicAmino Acids, DiaminoAmino Acids, SulfurSulfur CompoundsOrganic ChemicalsAmino Acids, NeutralAmino Acids, Aromatic

Results Point of Contact

Title
Hans C. Dreyer, PT, PhD
Organization
Human Physiology, University of Oregon

Study Officials

  • Hans C Dreyer, PT, PhD

    Assistant Professor, Department of Human Physiology

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
Yes
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
University of Oregon

Study Record Dates

First Submitted

September 25, 2008

First Posted

September 26, 2008

Study Start

June 1, 2008

Primary Completion

December 1, 2013

Study Completion

December 1, 2013

Last Updated

December 4, 2014

Results First Posted

December 4, 2014

Record last verified: 2014-12

Locations