Cellular Proteome From Leukocytes of Glaucoma Patients in Comparison With Patients With Alzheimer's Disease

NCT00691340 | Withdrawn

• 1 other identifier: 075-WUK-2008-002
• Study Type: observational
• Target: N/A
• Locations: 0 countries
• Sites: N/A

Brief Summary

Glaucoma is a worldwide leading cause of blindness. The key feature of this ocular neuropathy is characterized by an excavating optic nerve head. Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma. It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism. In earlier studies the investigators could demonstrate that the process of apoptosis is reflected in circulating leukocytes by different parameters, like differential mRNA expression and an increased fragmentation of the DNA. Such alterations point out a relationship between cellular stress and apoptotic events. Based on the results of mRNA-expression the investigators also expect alterations on the protein level. This study is, therefore, designed to characterize the proteome related to the proteins involved in cell death related pathways. Thus, the expression pattern of several proteins in leukocytes from patients with primary open angle glaucoma will be analyzed by techniques like Western-blot and tandem mass spectrometry. These samples will be compared with samples from healthy controls. In addition, they will also be compared with samples from patients with Alzheimer's disease. Since glaucoma is a neurodegenerative disease, these patients will be included as positive controls in this study.

Conditions

Alzheimer's Disease; Glaucoma

Keywords

POAG; Alzheimer's disease; cellular proteome; human leucocytes; vasospasm; Healthy subjects

Study Arms (4)

1

Control 1 (young subjects)

2

Control 2 (old subjects)

3

Glaucoma patients

4

Alzheimer patients

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Glaucoma patients Alzheimer's patients

You may qualify if:

• German native speakers
• Age between 18-85 years
• Primary open-angle glaucoma (POAG) with and without vasospasm

You may not qualify if:

• Any history of:
• Ocular or systemic diseases other than glaucoma or Alzheimer's disease
• Drug or alcohol abuse
• Any condition potentially interfering with the visual field results. Visual fields will be obtained from the chart.
• Mental impairment interfering with the ability to cooperate and understand the purpose of this study.

Sponsors & Collaborators

• University Hospital, Basel, Switzerland: lead
• University Hospital, Bonn: collaborator

Biospecimen

Retention: NONE RETAINED

plasma

MeSH Terms

Conditions

Alzheimer Disease; Glaucoma

Condition Hierarchy (Ancestors)

Dementia; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Tauopathies; Neurodegenerative Diseases; Neurocognitive Disorders; Mental Disorders; Ocular Hypertension; Eye Diseases

Study Officials

• Selim Orguel, MD

  University Hospital, Basel, Switzerland

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 3, 2008
• First Posted: June 5, 2008
• Study Start: June 1, 2008
• Primary Completion: August 1, 2011
• Study Completion: August 1, 2011
• Last Updated: March 10, 2015

Record last verified: 2015-03