NCT00537147

Brief Summary

West Nile (WN) virus infection is an emerging disease. Infection with WN virus may lead to paralysis, coma, and death. The purpose of this study is to determine the safety of and immune response to a two-dose regimen of a WN vaccine in healthy adults. The vaccine is based on a live attenuated vaccine developed against dengue virus.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
26

participants targeted

Target at P25-P50 for phase_1

Timeline
Completed

Started Mar 2008

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 27, 2007

Completed
1 day until next milestone

First Posted

Study publicly available on registry

September 28, 2007

Completed
5 months until next milestone

Study Start

First participant enrolled

March 1, 2008

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2009

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2009

Completed
Last Updated

January 3, 2013

Status Verified

December 1, 2012

Enrollment Period

1.3 years

First QC Date

September 27, 2007

Last Update Submit

December 31, 2012

Conditions

Keywords

Chimeric West Nile VirusWest Nile VirusDengue Virus

Outcome Measures

Primary Outcomes (2)

  • Frequency of vaccine-related adverse events, as classified by both intensity and severity through active and passive surveillance

    Throughout study

  • Immunogenicity, determined by anti-WN/DEN4 neutralizing antibody titer

    At study entry, Days 28 and 42 after first vaccination, and Days 180, 208, and 222 after second vaccination

Secondary Outcomes (3)

  • Assess the frequency, quantity, and duration of viremia after each dose of vaccine by dose cohort (10^4 or 10^5 PFU)

    Throughout study

  • Determine the number of vaccinees infected with WN/DEN4delta30 in each dose cohort (10^4 or 10^5 PFU)

    Throughout study

  • Compare the infectivity rates, safety, and immunogenicity between dose 1 and dose 2 within a cohort and between cohorts

    At study completion

Study Arms (3)

1

EXPERIMENTAL

1 vaccination of a 10\^4 plaque-forming units (PFU) dose of WN/DEN4delta30 vaccine administered as 0.5 ml subcutaneously in deltoid at study entry and Day 180.

Biological: WN/DEN4delta30 vaccine

2

EXPERIMENTAL

1 vaccination of a 10\^5 PFU dose of WN/DEN4delta30 vaccine administered as 0.5 ml subcutaneously in deltoid at study entry and Day 180.

Biological: WN/DEN4delta30 vaccine

3

PLACEBO COMPARATOR

1 vaccination of a placebo administered as 0.5 ml subcutaneously in deltoid at study entry and Day 180.

Biological: Placebo

Interventions

Live attenuated WN/DEN4delta30 vaccine (one of two doses)

12
PlaceboBIOLOGICAL

Placebo for WN/DEN4delta30 vaccine

3

Eligibility Criteria

Age18 Years - 50 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Good general health
  • Available for the duration of the trial
  • Willing to use acceptable forms of contraception for the duration of the study

You may not qualify if:

  • Clinically significant neurologic, heart, lung, liver, rheumatologic, autoimmune, or kidney disease
  • Behavioral, cognitive, or psychiatric disease that, in the opinion of the investigator, affects the ability of the volunteer to understand and cooperate with the study
  • Neutropenia (abnormally low neutrophil count)
  • Alcohol or drug abuse within 12 months prior to study entry
  • Elevated levels of alanine aminotransferase (ALT) and serum creatinine
  • History of severe allergic reaction or anaphylaxis
  • Severe asthma
  • HIV-1 infected
  • Hepatitis C virus infected
  • Hepatitis B surface antigen positive
  • Known immunodeficiency syndrome
  • Use of corticosteroids or immunosuppressive drugs within 30 days of study entry. Participants who have used topical or nasal corticosteroids are not excluded.
  • Live vaccine within 4 weeks prior to study entry
  • Killed vaccine within 2 weeks prior to study entry
  • Surgical removal of spleen
  • +6 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Center for Immunization Research, Johns Hopkins School of Public Health (DC Location)

Washington D.C., District of Columbia, 20037, United States

Location

Center for Immunization Research, Johns Hopkins School of Public Health (MD Location)

Baltimore, Maryland, 21205, United States

Location

Related Publications (2)

  • Chang GJ, Kuno G, Purdy DE, Davis BS. Recent advancement in flavivirus vaccine development. Expert Rev Vaccines. 2004 Apr;3(2):199-220. doi: 10.1586/14760584.3.2.199.

    PMID: 15056045BACKGROUND
  • Pletnev AG, Claire MS, Elkins R, Speicher J, Murphy BR, Chanock RM. Molecularly engineered live-attenuated chimeric West Nile/dengue virus vaccines protect rhesus monkeys from West Nile virus. Virology. 2003 Sep 15;314(1):190-5. doi: 10.1016/s0042-6822(03)00450-1.

    PMID: 14517072BACKGROUND

Related Links

MeSH Terms

Conditions

West Nile Fever

Condition Hierarchy (Ancestors)

Encephalitis, ArbovirusEncephalitis, ViralCentral Nervous System Viral DiseasesCentral Nervous System InfectionsInfectionsInfectious EncephalitisArbovirus InfectionsVector Borne DiseasesMosquito-Borne DiseasesVirus DiseasesRNA Virus InfectionsFlavivirus InfectionsFlaviviridae InfectionsEncephalitisBrain DiseasesCentral Nervous System DiseasesNervous System DiseasesNeuroinflammatory Diseases

Study Officials

  • Anna Durbin, M.D.

    Center for Immunization Research (CIR), Johns Hopkins School of Public Health

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 27, 2007

First Posted

September 28, 2007

Study Start

March 1, 2008

Primary Completion

June 1, 2009

Study Completion

June 1, 2009

Last Updated

January 3, 2013

Record last verified: 2012-12

Locations