NCT00322946

Brief Summary

Dengue fever, which is caused by dengue viruses, is a major health problem in tropical and subtropical regions of the world. The purpose of this study is to evaluate the safety and immune response to the dengue vaccine DEN4delta30-4995 in healthy adults.

Trial Health

80
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
84

participants targeted

Target at P75+ for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 4, 2006

Completed
4 days until next milestone

First Posted

Study publicly available on registry

May 8, 2006

Completed
8 months until next milestone

Study Start

First participant enrolled

January 1, 2007

Completed
2.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 1, 2009

Completed
Last Updated

August 7, 2009

Status Verified

November 1, 2008

Enrollment Period

2.6 years

First QC Date

May 4, 2006

Last Update Submit

August 5, 2009

Conditions

Keywords

Dengue FeverDengue VaccineDengue VirusDengue Hemorrhagic FeverDengue Shock Syndrome

Outcome Measures

Primary Outcomes (2)

  • Frequency of vaccine-related adverse events, graded by severity for each dose

    Throughout study

  • Immunogenicity of the rDEN4delta30-4995 vaccine against DEN4 virus by measurement of plaque reduction neutralization titers (PRNT)

    At Days 28 and 42

Secondary Outcomes (3)

  • Durability of antibody responses to DEN4 virus

    At Month 6

  • Frequency, quantity, and duration of viremia in each dose cohort studied based on the mean peak viremia, mean day onset of viremia, and mean duration of viremia of each dose cohort

    Throughout study

  • Number of vaccinees infected with the rDEN4delta30-4995 chimeric vaccine

    Throughout study

Study Arms (4)

1

EXPERIMENTAL

One subcutaneous vaccination with rDEN4delta30-4995 vaccine (10\^5 PFU dose) into the deltoid region of either arm.

Biological: rDEN4delta30-4995

2

EXPERIMENTAL

One subcutaneous vaccination with rDEN4delta30-4995 vaccine (10\^3 PFU dose) into the deltoid region of either arm. This arm will enroll after Arm 1.

Biological: rDEN4delta30-4995

3

EXPERIMENTAL

One subcutaneous vaccination with rDEN4delta30-4995 vaccine (10\^1 PFU dose) into the deltoid region of either arm. This arm will enroll after Arms 1 and 2.

Biological: rDEN4delta30-4995

4

PLACEBO COMPARATOR

One subcutaneous vaccination with placebo into the deltoid region of either arm.

Biological: Placebo

Interventions

Live attenuated rDEN4delta30-4995 vaccine (one of three doses)

123
PlaceboBIOLOGICAL

Placebo for rDEN4delta30-4995

4

Eligibility Criteria

Age18 Years - 50 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Adult males and non-pregnant females between 18 and 50 years of age
  • Good general health
  • Available for the duration of the study
  • Willing to use acceptable methods of contraception for the duration of the study

You may not qualify if:

  • Significant neurologic, cardiac, lung, liver, rheumatologic, autoimmune, or kidney disease
  • Behavioral, cognitive, or psychiatric disease that, in the opinion of the investigator, may interfere with the study
  • Significant laboratory abnormalities
  • Medical, work, or family problems as a result of alcohol or illegal drug use within 12 months prior to study entry
  • History of severe allergic reaction or anaphylaxis
  • Severe asthma
  • HIV-1 serotype infected
  • Hepatitis C virus (HCV) infected
  • Hepatitis B surface antigen positive
  • Immunodeficiency syndrome
  • Use of corticosteroids or immunosuppressive medications within 2 weeks prior to study entry. Individuals using topical or nasal corticosteroids are not excluded.
  • Live vaccine within 4 weeks prior to study entry
  • Killed vaccine within 2 weeks prior to study entry
  • Absence of spleen
  • Blood products within 6 months prior to study entry
  • +6 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Vanderbilt University School of Medicine

Nashville, Tennessee, 37232-2581, United States

Location

Related Publications (4)

  • Bhamarapravati N, Sutee Y. Live attenuated tetravalent dengue vaccine. Vaccine. 2000 May 26;18 Suppl 2:44-7. doi: 10.1016/s0264-410x(00)00040-2.

    PMID: 10821973BACKGROUND
  • Blaney JE Jr, Durbin AP, Murphy BR, Whitehead SS. Development of a live attenuated dengue virus vaccine using reverse genetics. Viral Immunol. 2006 Spring;19(1):10-32. doi: 10.1089/vim.2006.19.10.

    PMID: 16553547BACKGROUND
  • Durbin AP, Whitehead SS, McArthur J, Perreault JR, Blaney JE Jr, Thumar B, Murphy BR, Karron RA. rDEN4delta30, a live attenuated dengue virus type 4 vaccine candidate, is safe, immunogenic, and highly infectious in healthy adult volunteers. J Infect Dis. 2005 Mar 1;191(5):710-8. doi: 10.1086/427780. Epub 2005 Jan 27.

    PMID: 15688284BACKGROUND
  • Durbin AP, Karron RA, Sun W, Vaughn DW, Reynolds MJ, Perreault JR, Thumar B, Men R, Lai CJ, Elkins WR, Chanock RM, Murphy BR, Whitehead SS. Attenuation and immunogenicity in humans of a live dengue virus type-4 vaccine candidate with a 30 nucleotide deletion in its 3'-untranslated region. Am J Trop Med Hyg. 2001 Nov;65(5):405-13. doi: 10.4269/ajtmh.2001.65.405.

    PMID: 11716091BACKGROUND

MeSH Terms

Conditions

DengueSevere Dengue

Condition Hierarchy (Ancestors)

Mosquito-Borne DiseasesVector Borne DiseasesInfectionsArbovirus InfectionsVirus DiseasesFlavivirus InfectionsFlaviviridae InfectionsRNA Virus InfectionsHemorrhagic Fevers, Viral

Study Officials

  • Anna Durbin, MD

    Center for Immunization Research (CIR), Johns Hopkins School of Public Health

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH

Study Record Dates

First Submitted

May 4, 2006

First Posted

May 8, 2006

Study Start

January 1, 2007

Primary Completion

August 1, 2009

Last Updated

August 7, 2009

Record last verified: 2008-11

Locations