NCT00082446

Brief Summary

The overall goals of this study are to expand the available data on the safety and immunogenicity of MVA-BN in vaccinia-naive adults and to determine the optimum dose of MVA-BN to induce immune responses and attenuate Dryvax take reactions. Participants will include 90 healthy volunteers, ages 18-32 years. Participants will be randomly assigned to 1 of 6 study groups (groups A-F). Participants will be involved in study related procedures for up to 2 years. During this time, volunteers will return periodically for blood draws to check immune responses.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
91

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started May 2004

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2004

Completed
9 days until next milestone

First Submitted

Initial submission to the registry

May 10, 2004

Completed
1 day until next milestone

First Posted

Study publicly available on registry

May 11, 2004

Completed
3.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

August 1, 2007

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

August 1, 2007

Completed
Last Updated

December 19, 2014

Status Verified

September 1, 2008

Enrollment Period

3.3 years

First QC Date

May 10, 2004

Last Update Submit

December 18, 2014

Conditions

Keywords

Smallpox, vaccine

Outcome Measures

Primary Outcomes (1)

  • Adverse Events and side effects to the vaccines.

    Reactogenicity will be evaluated for a 2-week period post-vaccination at each time point and for the duration of study.

Secondary Outcomes (1)

  • Immunogenicity testing of antibody and cellular responses to the vaccines.

    Visit days 0, 14, 28, 42, 56, 112, 140, 182, 365, and 730.

Study Arms (6)

E

EXPERIMENTAL

Subject will receive an SC dose of MVA 1x10\^8 on day 0 and day 28. On day 112 subjects will receive a dose of placebo by scarification.

Biological: MVA Smallpox VaccineOther: Placebo

D

ACTIVE COMPARATOR

Subject will receive a SC dose of placebo on day 0 and day 28. On day 112 subjects will receive a dose of Dryvax® by scarification.

Biological: Live vaccinia virus vaccineOther: Placebo

C

EXPERIMENTAL

Subject will receive a SC dose of MVA 1x10\^8 on day 0 and day 28. On day 112 subjects will receive a dose of Dryvax® by scarification.

Biological: Live vaccinia virus vaccineBiological: MVA Smallpox Vaccine

B

EXPERIMENTAL

Subjects will receive a SC dose of MVA 5x10\^7 on day 0 and day 28. On day 112 subjects will receive a dose of Dryvax® by scarification.

Biological: Live vaccinia virus vaccineBiological: MVA Smallpox Vaccine

A

EXPERIMENTAL

Subjects will receive a SC dose of MVA 2x10\^7 on day 0 and day 28. On day 112 subjects will receive a dose of Dryvax® by scarification.

Biological: Live vaccinia virus vaccineBiological: MVA Smallpox Vaccine

F

EXPERIMENTAL

Subject will receive an IM dose of MVA 1x10\^8 on day 0 and day 28. On day 112 subjects will receive a dose of Dryvax® by scarification.

Biological: Live vaccinia virus vaccineBiological: MVA Smallpox Vaccine

Interventions

Dryvax®: 0.25 mL of vaccine will be administered by the standard route of scarification using a bifurcated needle on day 112 for Groups A, B, C, D and F.

ABCDF

Imvamune/MVA-BN 1x10\^8 will be administered intramuscularly to Group F on day 0 and day 28.

F
PlaceboOTHER

Group E will receive sterile saline placebo for injection via scarification on day 112.

E

Eligibility Criteria

Age18 Years - 32 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Ages 18-32.
  • Never received smallpox vaccination.
  • Read, signed and dated informed consent document.
  • Availability for follow-up for the planned duration of the study two years after first immunization.
  • Acceptable medical history by screening evaluation and limited physical examination.
  • For women, negative serum pregnancy test at screening and negative urine or serum pregnancy test within 24 hours prior to vaccination.
  • If the volunteer is female and of child bearing potential, she agrees to use acceptable contraception, and not become pregnant for at least 56 days after the last vaccination. A woman is considered of child bearing potential unless post-menopausal or surgically sterilized. \[Acceptable contraception methods are restricted to effective intrauterine devices (IUDs) or licensed hormonal products with use of method for a minimum of 30 days prior to vaccination.\] Women who are not sexually active must agree to use one of the acceptable contraception methods if they are of childbearing potential.
  • Negative ELISA for HIV.
  • ALT\<1.25 times institutional upper limit of normal.
  • Negative hepatitis B surface antigen and negative antibody to hepatitis C virus.
  • Negative urine glucose by dipstick or urinalysis.
  • Adequate renal function defined as a serum creatinine less than or equal to 1.4mg/dL for males and less than or equal to 1.2mg/dL for females; urine protein \< 30 mg/dL or none or trace proteinuria (by urinalysis or dipstick); and a calculated creatine clearance greater than or equal to 80 mL/min. based on the following formulas:
  • Males \[(140-age in years) X weight in kg\]/(72 X serum creatinine)
  • Females 0.85X\[(140-age in years) X weight in kg\]/(72 X serum creatinine)
  • ECG without clinical significance (e.g., all kinds of atrioventricular or intraventricular conditions or blocks such as complete left or right bundle branch block, AV-node block, QTc or PR prolongation, premature atrial contractions or other atrial arrhythmia, sustained ventricular arrhythmia, or 2 premature ventricular contractions (PVC) in a row, or ST elevation consistent with ischemia)
  • +1 more criteria

You may not qualify if:

  • History of immunodeficiency.
  • Typical vaccinia scar.
  • Known or suspected history of smallpox vaccination.
  • Military service prior to 1989 or after January 2003.
  • Known or suspected impairment of immunologic function including, but not limited to, clinically significant liver disease; diabetes mellitus; moderate to severe kidney impairment.
  • Malignancy, including squamous cell skin cancer or basal cell skin cancer at vaccination site or history of skin cancer at the vaccination site.
  • Active autoimmune disease. Persons with vitiligo or thyroid disease on thyroid replacement are not excluded.
  • History of keloid formation.
  • History of myocardial infarction, angina, congestive heart failure, cardiomyopathy, stroke or transient ischemic attack, or other heart condition under the care of a doctor.
  • History of an immediate family member (father, mother, brother or sister) who has had onset of ischemic heart disease before age 50 years.
  • Ten percent or greater risk of developing a myocardial infarction or coronary death within the next 10 years using the National Cholesterol Education Program's Risk Assessment Tool for Estimating Your 10-Year Risk of Having a Heart Attack, located at the following URL: http://hin.nhlbi.nih.gov/atpiii/calculator.asp. NOTE: This criterion applies only to volunteers 20 years of age and older.
  • Abnormal troponin I.
  • Use of immunosuppressive medication. Corticosteroid nasal sprays are permissible. Persons who have used topical steroid can be enrolled after their therapy is completed.
  • Medical or psychiatric condition or occupational responsibilities that preclude volunteer compliance with the protocol.
  • Any history of "illegal" injection drug use.
  • +17 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Saint Louis University

St Louis, Missouri, 63110, United States

Location

Related Publications (1)

  • Elizaga ML, Vasan S, Marovich MA, Sato AH, Lawrence DN, Chaitman BR, Frey SE, Keefer MC; MVA Cardiac Safety Working Group. Prospective surveillance for cardiac adverse events in healthy adults receiving modified vaccinia Ankara vaccines: a systematic review. PLoS One. 2013;8(1):e54407. doi: 10.1371/journal.pone.0054407. Epub 2013 Jan 17.

MeSH Terms

Conditions

Smallpox

Interventions

smallpox and monkeypox vaccine modified vaccinia ankara-bavarian nordic

Condition Hierarchy (Ancestors)

Poxviridae InfectionsDNA Virus InfectionsVirus DiseasesInfections

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 10, 2004

First Posted

May 11, 2004

Study Start

May 1, 2004

Primary Completion

August 1, 2007

Study Completion

August 1, 2007

Last Updated

December 19, 2014

Record last verified: 2008-09

Locations