The Effect of Aerobic Exercise on the Irisin/Klotho/Sirtuin-1/BDNF Axis

NCT07800156 | Not Yet Recruiting

• 1 other identifier: KAEK-120
• Study Type: interventional
• Target: 26
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to investigate the effects of an acute aerobic exercise on irisin, klotho, sirtuin-1, and BDNF levels in the healthy adults. The findings will help understand the physiological impacts of exercise on these biomarkers.

Conditions

Healthy Adult; Athlete

Outcome Measures

Primary Outcomes (4)

• Salivary Irisin Level

  Irisin concentration will be determined from a saliva sample using the ELISA technique.

  Baseline- immediately after exercise and 1 hours after exercise

• Salivary Klotho Level

  Klotho concentration will be determined from a saliva sample using the ELISA technique.

  Baseline- immediately after exercise and 1 hours after exercise

• Salivary Sirtuin Level

  Sirtuin concentration will be determined from a saliva sample using the ELISA technique.

  Baseline- immediately after exercise and 1 hours after exercise

• Salivary BDNF Level

  BDNF concentration will be determined from a saliva sample using the ELISA technique.

  Baseline- immediately after exercise and 1 hours after exercise

Secondary Outcomes (1)

• Saliva Flow Rate

  Baseline- İmmediately after exercise and 1 hour after exercise

Study Arms (2)

Experimental Group

EXPERIMENTAL

Aerobic exercise

Behavioral: Aerobic exercise

Control Group

NO INTERVENTION

No exercise

Interventions

Aerobic exercise BEHAVIORAL

Acute aerobic exercise until exhaustion

Experimental Group

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Being between the ages of 18 and 45
• Being in good health and having no diagnosis of any chronic disease
• Not having taken regular medication within the last 4 weeks
• Voluntary participation and providing written informed consent

You may not qualify if:

• Individuals with a history of cardiovascular, endocrine, metabolic, or renal disease
• Individuals with a history of active infection, musculoskeletal injury, or severe disability
• Individuals with any medical condition that might prevent participation in exercise
• Individuals unable to regularly participate in the exercise protocol throughout the study

Sponsors & Collaborators

• Cemre Didem Eyipinar: lead

Study Sites (1)

Gaziantep University Faculty of Sports Sciences

Gaziantep, Sehitkamil, 85142, Turkey (Türkiye)

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Central Study Contacts

Cemre Didem Eyipınar, PhD

CONTACT

+90 545 264 54 86; cemreeyipinar@gantep.edu.tr

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Research Assistant

Study Record Dates

• First Submitted: August 28, 2026
• First Posted: September 2, 2026
• Study Start: September 2, 2026
• Primary Completion (Estimated): April 2, 2027
• Study Completion (Estimated): May 2, 2027
• Last Updated: September 2, 2026

Record last verified: 2026-08

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)