The Effect of Guided Biofilm Therapy and Adjunctive Diode Laser on Periodontal Inflammation and Alveolar Bone Destruction in Patients With Stage 3 Periodontitis
Sponsor study identifier
Secondary identifiers
OTHER_GRANT · Atatürk University
interventional
60
1 country
1
Study Start
August 31, 2023
Actual (reported)Primary Completion
February 28, 2025
Actual (reported)Study Completion
February 28, 2025
Actual (reported)This randomized interventional study aims to compare the clinical and biochemical effects of conventional non-surgical periodontal therapy (NSPT), adjunctive diode laser therapy, and guided biofilm therapy (GBT) in patients with Stage 3 periodontitis. Participants are allocated to one of three treatment groups: conventional NSPT alone, NSPT combined with diode laser therapy, or NSPT combined with guided biofilm therapy. Clinical periodontal parameters, including plaque index, gingival index, probing pocket depth, clinical attachment level, and bleeding on probing, are evaluated before treatment and during follow-up. In addition, serum and gingival crevicular fluid samples are collected to determine the levels of inflammatory biomarkers, including IL-1β, IL-1 receptor antagonist (IL-1RA), sclerostin, and transforming growth factor-beta 1 (TGF-β1). The study evaluates whether adjunctive treatment modalities provide additional clinical and biological benefits compared with conventional non-surgical periodontal therapy alone.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
February 28, 2025
Actual (reported)Click on a node to explore related trials.
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Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
August 31, 2023
Actual (reported)February 28, 2025
Actual (reported)February 28, 2025
Actual (reported)August 24, 2026
Actual (reported)August 27, 2026
Actual (reported)Change in Clinical Attachment Level (CAL)
Change in clinical attachment level (CAL, mm) from baseline to 3 months after treatment. CAL was measured using a periodontal probe at six sites per tooth.
Baseline and 3 months
Change in IL-1β Levels in Gingival Crevicular Fluid
Change in gingival crevicular fluid IL-1β concentrations measured by ELISA from baseline to 3 months.
Baseline and 3 months
Change in Probing Pocket Depth (PPD)
Change in probing pocket depth (PPD, mm) measured using a periodontal probe at six sites per tooth.
Baseline and 3 months
Change in Gingival Index (GI)
Change in gingival inflammation assessed using the Gingival Index (GI).
Baseline and 3 months
Participants received conventional non-surgical periodontal therapy (NSPT), including full-mouth scaling and root planing using ultrasonic and hand instruments. No adjunctive treatment was administered in this group. Clinical and biochemical outcomes were evaluated during the follow-up period.
Participants received conventional non-surgical periodontal therapy followed by adjunctive diode laser therapy according to the study protocol. Clinical periodontal parameters and inflammatory biomarkers were evaluated during follow-up.
Conventional non-surgical periodontal therapy consisting of full-mouth scaling and root planing performed using ultrasonic and hand instruments according to the study protocol.
Department of Periodontology, Faculty of Dentistry, Ataturk University
Erzurum, 25240, Turkey (Türkiye)
Didem Özkal Eminoğlu
Ataturk University
60 participants (actual enrollment reported by the registry).
First Submitted
August 24, 2026
First Posted
August 27, 2026
Change in Plaque Index (PI)
Change in dental plaque accumulation assessed using the Plaque Index (PI).
Baseline and 3 months
Change in Bleeding on Probing (BOP)
Change in bleeding on probing (BOP) assessed during periodontal examination.
Baseline and 3 months
Change in Gingival Crevicular Fluid IL-1 Receptor Antagonist (IL-1RA) Levels
Change in gingival crevicular fluid IL-1 receptor antagonist (IL-1RA) concentrations measured using enzyme-linked immunosorbent assay (ELISA).
Baseline and 3 months
Change in Gingival Crevicular Fluid Sclerostin Levels
Change in gingival crevicular fluid sclerostin concentrations measured using enzyme-linked immunosorbent assay (ELISA).
Baseline and 3 months
Change in Gingival Crevicular Fluid Transforming Growth Factor-β1 (TGF-β1) Levels
Change in gingival crevicular fluid transforming growth factor-beta 1 (TGF-β1) concentrations measured using enzyme-linked immunosorbent assay (ELISA).
Baseline and 3 months
Change in Radiographic Alveolar Bone Level
Change in radiographic alveolar bone level assessed using standardized periapical radiographs.
Baseline and 3 months
Participants received conventional non-surgical periodontal therapy combined with guided biofilm therapy (GBT) using an air-polishing system according to the study protocol. Clinical periodontal parameters and inflammatory biomarkers were evaluated during follow-up.
Adjunctive diode laser therapy administered following conventional non-surgical periodontal therapy according to the study protocol.
Guided biofilm therapy performed using an erythritol powder air-polishing system as an adjunct to conventional non-surgical periodontal therapy according to the study protocol.
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Study Start
August 31, 2023
Actual (reported)Primary Completion
February 28, 2025
Actual (reported)Study Completion
February 28, 2025
Actual (reported)Last Updated
August 27, 2026
Record last verified: 2026-08
De-identified individual participant data underlying the results reported in the published article, including demographic characteristics, clinical periodontal measurements, radiographic measurements, and inflammatory biomarker data (IL-1β, IL-1 receptor antagonist \[IL-1RA\], sclerostin, and transforming growth factor-beta 1 \[TGF-β1\]), will be shared.
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Source: ClinicalTrials.gov.
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