Advanced Platelet-Rich Fibrin Plus in Nonsurgical Periodontal Therapy

NCT07782736 | Completed

• 1 other identifier: 3518/DHYD-HDDD
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this randomized, controlled, split-mouth clinical trial was to determine whether adding advanced platelet-rich fibrin plus (A-PRF+), an autologous fibrin membrane prepared from the participant's own blood, to routine nonsurgical periodontal therapy could improve the healing of periodontal pockets. Adults with periodontitis and periodontal sites with a probing pocket depth of 5 mm or greater and bleeding on probing were enrolled. For each participant, one side of the mouth was randomly assigned to receive scaling and root planing plus A-PRF+ membrane placement, while the other side received scaling and root planing alone. Periodontal probing depth, clinical attachment level, gingival recession, gingival index, bleeding on probing, and plaque index were assessed at baseline and after 4 and 8 weeks.

Conditions

Periodontitis

Keywords

Advanced Platelet-Rich Fibrin Plus; A-PRF+; Nonsurgical Periodontal Therapy; Split-Mouth Design

Outcome Measures

Primary Outcomes (2)

• Probing Pocket Depth

  Probing pocket depth was measured in millimeters using a periodontal probe, from the gingival margin to the base of the periodontal pocket. Lower values indicate shallower periodontal pockets.

  Baseline, 4 weeks, and 8 weeks after treatment

• Clinical Attachment Level

  Clinical attachment level was measured in millimeters using a periodontal probe, from the cementoenamel junction to the base of the periodontal pocket. Lower values indicate less clinical attachment loss.

  Baseline, 4 weeks, and 8 weeks after treatment

Secondary Outcomes (4)

• Gingival Recession

  Baseline, 4 weeks, and 8 weeks after treatment

• Gingival Index

  Baseline, 4 weeks, and 8 weeks after treatment

• Bleeding on Probing

  Baseline, 4 weeks, and 8 weeks after treatment

• Plaque Index

  Baseline, 4 weeks, and 8 weeks after treatment

Study Arms (2)

Intervention Side: Scaling and Root Planing Plus A-PRF+

EXPERIMENTAL

Eligible periodontal pockets on the randomly assigned intervention side received scaling and root planing followed by placement of an autologous A-PRF+ membrane.

Procedure: Scaling and Root Planing; Biological: Advanced Platelet-Rich Fibrin Plus

Control Side: Scaling and Root Planing Alone

ACTIVE COMPARATOR

Eligible periodontal pockets on the contralateral control side received scaling and root planing without A-PRF+ membrane placement.

Procedure: Scaling and Root Planing

Interventions

Scaling and Root Planing PROCEDURE

Nonsurgical mechanical periodontal treatment was performed to remove supra- and subgingival plaque and calculus and to debride the root surfaces.

Control Side: Scaling and Root Planing Alone; Intervention Side: Scaling and Root Planing Plus A-PRF+

Advanced Platelet-Rich Fibrin Plus BIOLOGICAL

The autologous A-PRF+ membrane was prepared from venous blood collected without anticoagulants or additives and centrifuged at 1,300 rpm for 8 minutes. Following mechanical debridement and routine pocket irrigation, intervention pockets were rinsed with sterile saline before A-PRF+ membrane placement. The membrane was placed into eligible periodontal pockets and stabilized using an adhesive periodontal patch.

Intervention Side: Scaling and Root Planing Plus A-PRF+

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Participant-level criteria:
• Adults aged 18 years or older.
• Diagnosis of periodontitis according to the 2017 World Workshop criteria.
• At least one eligible periodontal tooth/site in each half-mouth.
• Provision of written informed consent and agreement to attend the scheduled follow-up visits.
• Site-level criteria:
• Probing pocket depth of 5 mm or greater at baseline.
• Clinical attachment loss of 3 mm or greater at baseline.
• Positive bleeding on probing at baseline.

You may not qualify if:

• Participant-level criteria:
• Current use of tobacco or related products.
• Systemic conditions closely associated with periodontitis or treatment response, including diabetes mellitus, rheumatoid arthritis, or hematologic disease.
• Pregnancy or lactation.
• Periodontal surgery within the preceding 6 months.
• Use of medications affecting blood coagulation.
• Abnormal preintervention complete blood count, prothrombin time, or activated partial thromboplastin time.
• Poor oral hygiene.
• Failure to attend protocol-specified visits.
• Tooth/site-level criteria:
• Untreated dental caries or pulpal disease.
• Teeth undergoing active orthodontic treatment.
• Teeth with defective restorations.
• Third molars.
• Teeth with mobility grade II or greater.

Sponsors & Collaborators

• University of Medicine and Pharmacy at Ho Chi Minh City: lead

Study Sites (1)

Specialty Dental Clinic, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City

Ho Chi Minh City, Vietnam

Location

MeSH Terms

Conditions

Periodontitis

Interventions

Tooth Exfoliation; Root Planing

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Intervention Hierarchy (Ancestors)

Dental Physiological Phenomena; Digestive System and Oral Physiological Phenomena; Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Masking Details: Participants were not informed which side of the mouth was assigned to the intervention or control treatment. Clinical periodontal outcomes were assessed by a calibrated examiner who was unaware of the side allocation. The clinician providing the treatment could not be masked because of the nature of the intervention.
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Doctor of Dental Surgery

Model Details: Randomized within-participant split-mouth design. Each participant received both treatments concurrently: scaling and root planing plus A-PRF+ on the randomly assigned intervention side and scaling and root planing alone on the control side.

Study Record Dates

• First Submitted: August 20, 2026
• First Posted: August 24, 2026
• Study Start: October 3, 2025
• Primary Completion: April 24, 2026
• Study Completion: April 24, 2026
• Last Updated: August 24, 2026

Record last verified: 2026-08

Data Sharing

• IPD Sharing: Will not share

Locations

VN (1)