NCT07692490

Brief Summary

The ARANMP-LT study is a multicenter, open-label, randomized, parallel-group, non-inferiority clinical trial designed to evaluate whether avoiding graft recooling after normothermic machine perfusion (NMP) improves early outcomes following liver transplantation. Although graft recooling before implantation is common practice, experimental and preliminary clinical evidence suggests that abrupt temperature changes may contribute to ischemia-reperfusion injury. Adult recipients of deceased-donor liver grafts preserved with NMP or sequential hypothermic-normothermic machine perfusion (SHNMP) will be randomized in a 1:1 ratio to either standard graft implantation with recooling using cold preservation solution (control group) or implantation without recooling using a room-temperature 5% albumin flush immediately before reperfusion (intervention group). The primary objective is to compare the incidence of Early Allograft Dysfunction (EAD), defined according to the Olthoff criteria, between the two treatment groups. Secondary objectives include evaluation of histopathological ischemia-reperfusion injury using the Suzuki score, vascular and biliary complications, primary non-function, post-reperfusion syndrome, patient and graft survival, length of intensive care unit and hospital stay, perioperative transfusion requirements, and safety outcomes. A total of 170 participants will be enrolled across multiple Italian liver transplant centers and followed for 90 days after transplantation.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
170

participants targeted

Target at P75+ for not_applicable

Timeline
26mo left

Started Jul 2026

Typical duration for not_applicable

Geographic Reach
1 country

14 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress4%
Jul 2026Oct 2028

First Submitted

Initial submission to the registry

July 2, 2026

Completed
5 days until next milestone

Study Start

First participant enrolled

July 7, 2026

Completed
2 days until next milestone

First Posted

Study publicly available on registry

July 9, 2026

Completed
2.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

October 6, 2028

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

October 6, 2028

Last Updated

July 14, 2026

Status Verified

July 1, 2026

Enrollment Period

2.3 years

First QC Date

July 2, 2026

Last Update Submit

July 10, 2026

Conditions

Keywords

Liver TransplantationNormothermic Machine PerfusionIschemia-Reperfusion InjuryEarly Allograft DysfunctionOrgan PreservationLiver Graft

Outcome Measures

Primary Outcomes (1)

  • Early Allograft Dysfunction (EAD)

    Incidence of Early Allograft Dysfunction (EAD), defined according to the Olthoff criteria as bilirubin ≥10 mg/dL or INR ≥1.6 on postoperative day 7, or AST/ALT \>2000 IU/L within the first 7 postoperative days.

    Within 7 days after liver transplantation

Study Arms (2)

No Recooling

EXPERIMENTAL

No Recooling Before Liver Implantation

Procedure: No Recooling Before Liver Implantation

Traditional Recooling

ACTIVE COMPARATOR

Traditional Recooling Before Liver Implantation

Procedure: Traditional Recooling Before Liver Implantation

Interventions

After completion of normothermic machine perfusion, the liver graft is flushed with 5% albumin solution at room temperature (20-25°C) and implanted without recooling. The graft remains at near-normothermic temperature throughout the transition from ex vivo perfusion to in situ implantation, avoiding the conventional cold preservation flush before reperfusion.

No Recooling

After completion of normothermic machine perfusion, the liver graft undergoes the standard preservation procedure consisting of a cold preservation solution flush before implantation, resulting in graft recooling prior to reperfusion. The remainder of the transplantation procedure follows standard clinical practice.

Traditional Recooling

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age ≥18 years.
  • Listed for primary liver transplantation at the participating institution.
  • Assigned a liver graft preserved with normothermic machine perfusion or sequential hypothermic- normothermic machine perfusion (SHNMP).

You may not qualify if:

  • Pediatric patients (age \< 18 years)
  • Patients undergoing re-transplantation (history of previous liver transplantation)
  • Patients with acute liver failure
  • Patients undergoing multi-organ transplantation (e.g., liver-kidney, liver-heart)
  • Temporarily unconscious patients
  • Patients unable to freely express consent.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (14)

Università Politecnica delle Marche, AUO delle Marche

Ancona, 60126, Italy

NOT YET RECRUITING

Azienda Ospedaliero-Universitaria Consorziale Policlinico di Bari - Ospedale Giovanni XXIII

Bari, 70124, Italy

NOT YET RECRUITING

ASST Papa Giovanni XXIII

Bergamo, 24127, Italy

NOT YET RECRUITING

IRCCS Azienda Ospedaliero-Universitaria di Bologna

Bologna, 40138, Italy

NOT YET RECRUITING

Ospedale Università Policlinico San Martino - IRCCS

Genova, 16132, Italy

NOT YET RECRUITING

Università di Modena e Reggio Emilia

Modena, 41124, Italy

NOT YET RECRUITING

Azienda Ospedale-Università di Padova

Padova, 35128, Italy

NOT YET RECRUITING

IRCCS ISMETT - Istituto Mediterraneo per i Trapianti e le Terapie ad Alta Specializzazione

Palermo, 90127, Italy

RECRUITING

Azienda Ospedaliero Universitaria Pisana

Pisa, 56126, Italy

NOT YET RECRUITING

Fondazione PTV - Policlinico Tor Vergata

Roma, 00133, Italy

NOT YET RECRUITING

Azienda Ospedaliera San Camillo Forlanini

Roma, 00152, Italy

NOT YET RECRUITING

Azienda Ospedaliero-Universitaria Policlinico Umberto I - Sapienza Università di Roma

Roma, 00161, Italy

NOT YET RECRUITING

Fondazione Policlinico "Agostino Gemelli" IRCCS

Roma, 00168, Italy

NOT YET RECRUITING

Azienda Ospedaliera Universitaria Integrata Verona

Verona, 37126, Italy

NOT YET RECRUITING

MeSH Terms

Conditions

Reperfusion Injury

Condition Hierarchy (Ancestors)

Vascular DiseasesCardiovascular DiseasesPostoperative ComplicationsPathologic ProcessesPathological Conditions, Signs and Symptoms

Central Study Contacts

Salvatore Gruttadauria

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 2, 2026

First Posted

July 9, 2026

Study Start

July 7, 2026

Primary Completion (Estimated)

October 6, 2028

Study Completion (Estimated)

October 6, 2028

Last Updated

July 14, 2026

Record last verified: 2026-07

Data Sharing

IPD Sharing
Will not share

Locations