Comparison of Ultrasonic vs 980-nm Diode Laser Irrigant Activation for Reducing Residual Bacterial DNA in Necrotic Teeth

NCT07607028 | Completed

• 1 other identifier: No. 265/KEPK FKG-RSGMP UH/EA/I
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

This randomized clinical trial aimed to compare residual oral bacterial DNA detection after ultrasonic irrigant activation and 980-nm diode laser irrigant activation during root canal treatment of necrotic single-rooted teeth. Sixteen patients requiring endodontic treatment were randomly assigned into two groups: ultrasonic activation or 980-nm diode laser activation. Root canals were prepared using standardized chemomechanical procedures and irrigated with 3% sodium hypochlorite activated by the assigned method. Microbial samples were collected before treatment and after irrigant activation. Bacterial DNA was identified using 16S rRNA polymerase chain reaction (PCR) sequencing. The primary outcome was the presence or absence of detectable oral bacterial DNA after treatment, while the secondary outcome was the taxonomic pattern of detected bacteria before and after activation. The study was designed to explore the comparative antibacterial effects of ultrasonic and diode laser activation in infected root canals.

Conditions

Pulp Necrosis; Root Canal Infection

Keywords

Pulp necrosis; Diode laser; Ultrasonic activation; Root canal irrigation; 16S rRNA PCR; Endodontic disinfection; Root canal infection; Sodium hypochlorite

Outcome Measures

Primary Outcomes (1)

• Presence of Detectable Oral Bacterial DNA After Irrigant Activation

  Detection of residual oral bacterial DNA in root canals after irrigant activation using ultrasonic activation or 980-nm diode laser activation, assessed by 16S rRNA PCR sequencing.

  Immediately after completion of irrigant activation during root canal treatment.

Study Arms (2)

Ultrasonic Activation

EXPERIMENTAL

Participants received root canal treatment with irrigant activation using ultrasonic activation in combination with 3% sodium hypochlorite irrigation. Ultrasonic activation was performed at 45 kHz for 20 seconds per cycle for three cycles with irrigant renewal between cycles

Device: Ultrasonic Activation

980-nm Diode Laser Activation

EXPERIMENTAL

Participants received root canal treatment with irrigant activation using a 980-nm diode laser in combination with 3% sodium hypochlorite irrigation. Laser activation was performed at 1.5 W for 20 seconds per cycle for three cycles with irrigant renewal between cycles.

Device: 980-nm Diode Laser Activation

Interventions

Ultrasonic Activation DEVICE

Ultrasonic irrigant activation was performed during root canal treatment using an ultrasonic device at 45 kHz for 20 seconds per cycle for three cycles with 3% sodium hypochlorite irrigation.

Ultrasonic Activation

980-nm Diode Laser Activation DEVICE

Diode laser irrigant activation was performed during root canal treatment using a 980-nm diode laser at 1.5 W for 20 seconds per cycle for three cycles with 3% sodium hypochlorite irrigation.

980-nm Diode Laser Activation

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Adults aged 18 years or older
• Patients requiring endodontic treatment for mature single-rooted teeth diagnosed with pulp necrosis
• Physically and mentally able to undergo treatment
• No use of analgesics or antibiotics within one week before treatment
• Teeth without clinical or radiographic evidence of apical periodontitis
• Diagnosis confirmed by clinical examination, pulp vitality testing, and periapical radiography

You may not qualify if:

• Teeth with mobility
• Periodontal pockets greater than 4 mm
• Root fracture or fracture during treatment
• Internal or external root resorption
• Canal calcification or canal obliteration
• Patients with systemic conditions requiring antibiotic prophylaxis for routine dental treatment
• Recent antimicrobial exposure that could influence microbial findings

Sponsors & Collaborators

• Hasanuddin University: lead

Study Sites (1)

Dental and Oral Hospital Hasanuddin University

Makassar, South Sulawesi, 90245, Indonesia

Location

Related Publications (2)

• Siqueira JF Jr, Rocas IN. Present status and future directions: Microbiology of endodontic infections. Int Endod J. 2022 May;55 Suppl 3:512-530. doi: 10.1111/iej.13677. Epub 2022 Jan 13.

  PMID: 34958494 BACKGROUND

• Jambagi N, Kore P, Dhaded NS, Patil SA, Shankar M. Comparison of Antimicrobial Efficacy of Diode Laser, Ultrasonic Activated and Conventional Irrigation with 2.5% NaOCl during RCT: An Interventional Study. J Contemp Dent Pract. 2021 Jun 1;22(6):669-673.

  PMID: 34393125 BACKGROUND

MeSH Terms

Conditions

Dental Pulp Necrosis

Condition Hierarchy (Ancestors)

Dental Pulp Diseases; Tooth Diseases; Stomatognathic Diseases; Necrosis; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Masking Details: None (Open Label) Not applicable. No masking was applied in this study
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Endodontic Resident

Model Details: Participants were randomly assigned into two parallel groups to receive root canal irrigant activation using either ultrasonic activation or 980-nm diode laser activation during endodontic treatment of necrotic single-rooted teeth.

Study Record Dates

• First Submitted: May 19, 2026
• First Posted: May 26, 2026
• Study Start: October 13, 2025
• Primary Completion: February 28, 2026
• Study Completion: March 31, 2026
• Last Updated: May 26, 2026

Record last verified: 2026-05

Data Sharing

• IPD Sharing: Will not share

Locations

ID (1)