Profiling Gingival Crevicular Fluid β-Catenin Level With 8-OHdG and Total Antioxidants Capacity During Healing of Periodontal Pockets: A 3-month Clinical Trial

NCT07596667 | Not Yet Recruiting

• 1 other identifier: BabylonU
• Study Type: interventional
• Target: 21
• Locations: 0 countries
• Sites: N/A

Brief Summary

Background: To profile gingival crevicular fluid (GCF) β-Catenin, 8-OHdG, and total antioxidants capacity (TAC) in periodontitis patients during healing of periodontal pockets following non-surgical periodontal therapy (NSPT).Methods: Periodontitis patients (n = 21) will included in this clinical trial. Clinical periodontal parameters will recorded and GCF samples will collected from randomly selected 4-6 mm periodontal pockets at baseline (T0) as well as 4 weeks (T1) and 12 weeks (T2) after NSPT. GCF levels of β-catenin, 8-OHdG, and TAC will be assayed by enzyme linked immunosorbent assay (ELISA).

Conditions

Periodontitis, Adult; Periodontal Diseases

Keywords

periodontitis; non surgical periodontal therapy

Outcome Measures

Primary Outcomes (3)

• periodontal pocket depth

  periodontal pocket depth reduction

  3 months

• measurement of probing pocket depth (PPD)

  measuring probing pocket depth (PPD)

  3 months

• measurement of clinical attachment loss (CAL)

  clinical attachment loss (CAL)

  3 months

Secondary Outcomes (1)

• GCF levels of β-catenin, 8-OHdG, and TAC will be assayed by enzyme linked immunosorbent assay (ELISA) in which all have the same measuring units

  3 months

Study Arms (1)

periodontitis patients with pocket depth

EXPERIMENTAL

Procedure: scaling and root planing

Interventions

scaling and root planing PROCEDURE

Periodontitis patients (n = 21) will included in this clinical trial. Clinical periodontal parameters will recorded and GCF samples will collected from randomly selected 4-6 mm periodontal pockets at baseline (T0) as well as 4 weeks (T1) and 12 weeks (T2) after NSPT. GCF levels of β-catenin, 8-OHdG, and TAC will be assayed by enzyme linked immunosorbent assay (ELISA).

periodontitis patients with pocket depth

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• patients over 18 years old
• patients with periodontal pockets depth more than 4 mm

You may not qualify if:

• Pregnant women
• patients taking antibiotic within the last three months
• Patients with systemic disease ( Diabetes mellitus)

Sponsors & Collaborators

• Zeyad Nazar: lead
• Babylon University: collaborator

Related Publications (1)

• Vitkov L, Singh J, Schauer C, Minnich B, Krunic J, Oberthaler H, Gamsjaeger S, Herrmann M, Knopf J, Hannig M. Breaking the Gingival Barrier in Periodontitis. Int J Mol Sci. 2023 Feb 25;24(5):4544. doi: 10.3390/ijms24054544.

  PMID: 36901974 BACKGROUND

MeSH Terms

Conditions

Chronic Periodontitis; Periodontal Diseases; Periodontitis

Interventions

Tooth Exfoliation; Root Planing

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Dental Physiological Phenomena; Digestive System and Oral Physiological Phenomena; Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Assistant Professor

Model Details: Periodontitis patients (n = 21) will be included in this clinical trial. Clinical periodontal parameters will be recorded and GCF samples will be collected from randomly selected 4-6 mm periodontal pockets at baseline (T0) as well as 4 weeks (T1) and 12 weeks (T2) after NSPT. GCF levels of β-catenin, 8-OHdG, and TAC will be assayed by enzyme linked immunosorbent assay (ELISA).

Study Record Dates

• First Submitted: May 7, 2026
• First Posted: May 19, 2026
• Study Start (Estimated): July 15, 2026
• Primary Completion (Estimated): November 15, 2026
• Study Completion (Estimated): December 1, 2026
• Last Updated: May 19, 2026

Record last verified: 2026-05