Molecular Insights Into Post-Cardiac Arrest Brain Injury Via CSF Multi-Omics

NCT07481396 | Recruiting

• 2 other identifiers: CNUH-2025-07-044-003RS-2025-00554697
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this study is to uncover the molecular mechanisms responsible for secondary brain injury in patients with post-cardiac arrest syndrome by analyzing cerebrospinal fluid (CSF) using multi-omics techniques. The main question this study aims to answer is: Which genome-, transcriptome-, proteome-, and metabolome-level changes in CSF are associated with secondary brain injury after cardiac arrest? To address this question, CSF samples collected from post-cardiac arrest patients will undergo multi-omics analyses. Identified molecular pathways will be used to screen existing drug databases and generate new therapeutic candidates through computational modeling and compound synthesis. These findings will provide the scientific foundation needed to design and implement future preclinical experiments using cardiac arrest animal models.

Conditions

Cardiac Arrest (CA); Cerebrospinal Fluid; Multiomics; Hypoxic-Ischemic Brain Injury

Outcome Measures

Primary Outcomes (1)

• Neurological outcome

  Neurological outcome assessed using the Cerebral Performance Category (CPC) scale (range 1-5; lower scores indicate better neurological function).

  at discharge (assessed up to 3 days), 3 months after return of spontaneous circulation (ROSC)

Study Arms (2)

Favorable neurological outcome

The favorable neurological outcome group is classified as patients with a CPC score of 1 or 2 upon long-term follow-up (at discharge, 3 months after post-resuscitation)

Unfavorable neurological outcome

The favorable neurological outcome group is classified as patients with a CPC score of 3 to 5 upon long-term follow-up (at discharge, 3 months after post-resuscitation)

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The study population for this research consists of a highly specific and clinically critical group of patients: Adults who have experienced out-of-hospital or in-hospital cardiac arrest and successfully regained spontaneous circulation (ROSC), but remain at high risk for secondary brain injury.

You may qualify if:

• Patients receiving post-resuscitation care after out-of-hospital cardiac arrest for secondary brain injury treatment.
• Patients without contraindications for lumbar puncture catheter insertion for cerebrospinal fluid (CSF) collection. This includes the absence of:
• Uncontrolled diabetes.Coagulation disorders.
• Thrombocytopenia (platelet count $\< 100,000).
• A history of cirrhosis diagnosis.
• Current receipt of low molecular weight heparin.
• Current use of platelet inhibitors.
• A history of posterior spinal fusion that may interfere with catheter insertion.
• Local skin infection or rash at the puncture site.
• Signs of systemic infection or sepsis.
• A history of lumbar puncture within the past 6 hours.

You may not qualify if:

• Cerebral Edema: Patients with evidence of cerebral edema on a brain computed tomography (CT) scan performed immediately after spontaneous circulation recovery.
• Patients who underwent extracorporeal membrane oxygenation (ECMO).
• Patients who could not maintain integrated therapy for more than 24 hours after cardiac arrest.
• Patients with a history of acute or chronic brain disease.

Sponsors & Collaborators

• Chungnam National University Hospital: lead

Study Sites (1)

Chungnam National University Hospital

Daejeon, Jung-gu, 35015, South Korea

RECRUITING

Related Publications (4)

• 5. Edgren E, Enblad P, Grenvik A, et al. Cerebral blood flow and metabolism after cardiopulmonary resuscitation. A pathophysiologic and prognostic positron emission tomography pilot study. Resuscitation 2003;57:161-70.

  BACKGROUND

• 4. Buunk G, van der Hoeven JG, Meinders AE. Cerebral blood flow after cardiac arrest. Neth J Med 2000;57:106-12.

  BACKGROUND

• Madl C, Holzer M. Brain function after resuscitation from cardiac arrest. Curr Opin Crit Care. 2004 Jun;10(3):213-7. doi: 10.1097/01.ccx.0000127542.32890.fa.

  PMID: 15166839 BACKGROUND

• 1. Wiklund LM, Miclescu A, Semenas E, Rubertsson S, Sharma HS. Central nervoustissue damage after hypoxia and reperfusion in conjunction with cardiac arrest and cardiopulmonary resuscitation: mechanisms of action and possibilities for mitigation. Int Rev Neruobiol 2012;102:173-87.

  BACKGROUND

Biospecimen

Retention: SAMPLES WITHOUT DNA

The primary and exclusive biological sample collected and retained for the multi-omics analysis is the cerebrospinal fluid and serum.

MeSH Terms

Conditions

Heart Arrest; Hypoxia-Ischemia, Brain

Condition Hierarchy (Ancestors)

Heart Diseases; Cardiovascular Diseases; Brain Ischemia; Cerebrovascular Disorders; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Hypoxia, Brain; Vascular Diseases; Hypoxia; Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Central Study Contacts

Changshin Kang, MD. PhD

CONTACT

+821089928386; rosc@cnu.ac.kr

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 3 Months
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor

Study Record Dates

• First Submitted: November 27, 2025
• First Posted: March 18, 2026
• Study Start: December 1, 2025
• Primary Completion (Estimated): November 30, 2027
• Study Completion (Estimated): February 28, 2028
• Last Updated: March 18, 2026

Record last verified: 2026-03

Locations

KR (1)