Periodontal Status and Cytokine Levels in Patients With Non-Infectious Uveitis

NCT07480291 | Completed

• 1 other identifier: NIU-Perio-2020
• Study Type: observational
• Target: 52
• Locations: 1 country
• Sites: 1

Brief Summary

This cross-sectional observational study aimed to evaluate periodontal clinical parameters and inflammatory cytokine levels in gingival crevicular fluid (GCF), saliva, and serum in patients with non-infectious uveitis (NIU) compared with systemically healthy individuals. Twenty-five patients diagnosed with non-infectious uveitis and twenty-seven age- and sex-matched healthy controls were included. Clinical periodontal parameters including probing pocket depth, clinical attachment loss, plaque index, gingival index, and bleeding on probing were recorded. Biological samples including saliva, gingival crevicular fluid, and serum were collected. Levels of TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17 were measured using ELISA. The study aimed to investigate whether periodontal inflammatory status and systemic cytokine profiles are associated with non-infectious uveitis.

Conditions

Non-infectious Uveitis; Periodontal Disease

Keywords

Cytokines; Gingival crevicular fluid; serum; saliva; Periodontal status; non-infectious uveitis

Outcome Measures

Primary Outcomes (1)

• IL-10 concentration in serum (pg/L)

  IL-10 concentration was measured in serum samples using enzyme-linked immunosorbent assay (ELISA). Cytokine concentrations were calculated based on standard curves and expressed as picograms per liter (pg/L).

  Baseline

Secondary Outcomes (20)

• TNF-α concentration in gingival crevicular fluid (ng/sec)

  Baseline

• TNF-α concentration in saliva (ng/min)

  Baseline

• TNF-α concentration in serum (ng/L)

  Baseline

• IL-1β concentration in gingival crevicular fluid (pg/sec)

  Baseline

• IL-1β concentration in saliva (pg/min)

  Baseline

Study Arms (2)

Non-infectious Uveitis Group

Participants diagnosed with non-infectious uveitis who underwent periodontal examination and provided saliva, gingival crevicular fluid, and serum samples for cytokine analysis.

Healthy Control Group

Systemically healthy individuals without ocular inflammatory disease who underwent periodontal examination and biological sample collection.

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

This cross-sectional study included adult individuals diagnosed with non-infectious uveitis (NIU) and systemically healthy controls. Participants were recruited from the outpatient clinic of the Department of Ophthalmology at Selcuk University. All participants underwent comprehensive periodontal examination and biofluid sampling, including gingival crevicular fluid, saliva, and serum, for cytokine analysis.

You may qualify if:

• Participants aged 18 years or older.
• Patients diagnosed with non-infectious uveitis according to the
• Standardization of Uveitis Nomenclature (SUN) criteria.
• Presence of at least 20 natural teeth.
• Individuals who agreed to participate in the study and provided informed consent.

You may not qualify if:

• Presence of infectious uveitis.
• Pregnancy or lactation.
• Use of antibiotics, anti-inflammatory drugs, or immunosuppressive therapy within the previous 6 months.
• History of periodontal treatment within the previous 6 months.
• Presence of systemic diseases known to affect periodontal status.
• Current smokers.

Sponsors & Collaborators

• Selcuk University: lead

Study Sites (1)

Selcuk University Faculty of Dentistry

Konya, Konya, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

Saliva, gingival crevicular fluid, and serum samples were collected from participants for cytokine analysis.

MeSH Terms

Conditions

Periodontal Diseases

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases

Study Officials

• Duygu durmaz, DDS,MSc

  Pendik Oral and Dental Health Hospital, Istanbul, Turkey

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: DDS, MSc, Periodontist

Study Record Dates

• First Submitted: March 11, 2026
• First Posted: March 18, 2026
• Study Start: October 1, 2020
• Primary Completion: August 31, 2021
• Study Completion: August 31, 2021
• Last Updated: March 18, 2026

Record last verified: 2026-03

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)