Short-Term Broccoli Supplementation and Acute Oxidative Stress Recovery

NCT07454265 | Completed

• 1 other identifier: BI-TRS (M)-2024-703
• Study Type: interventional
• Target: 17
• Locations: 1 country
• Sites: 1

Brief Summary

The study aimed to evaluate the effects of short-term broccoli powder supplementation on metabolically demanding exercise performance, muscle power, and blood lactate recovery. It also investigated broccoli powder-derived sulforaphane bioavailability and its effects in attenuating exercise-induced oxidative stress. Seventeen healthy males (age 23.8 ± 4.9 years, height 182.3 ± 6.1 cm, weight 80.0 ± 12.8 kg), in a double-blind crossover design, three weeks apart, consumed ten standard doses of either broccoli powder or spinach powder as a placebo over a period of 2 weeks. They then performed a maximal progressive cycling task with concomitant analysis of expired gas composition. Plasma malondialdehyde (MDA) level was measured before and 60 min after the completion of the task, and blood lactate and muscle power (counter-movement vertical jump (CMJ) performance) were measured before and up to 60 min after exercise.

Conditions

Healthy Adult Male

Keywords

Sulforaphane; Bioavailability; Lactate; VO₂max

Outcome Measures

Primary Outcomes (4)

• Sulforaphane, mg/24hours

  Urine was collected over a 24-hours period following ingestion of the first supplementation dose in both conditions, and sulforaphane was subsequently extracted and analyzed up to one week after study completion.

• Plasma malondialdehyde concentration, nmol/mL

  Blood sample was collected before incremental cycling and 1 hour post-test in both conditions and plasma malondialdehyde concentration was subsequently analyzed up to one week after study completion.

• Peak power output, Watts

  During each experimental condition (at peak exercise during incremental ramp cycling test)

• Maximal oxygen uptake, mL/min/kg

  During each experimental condition (at peak exercise during incremental ramp cycling test)

Secondary Outcomes (3)

• Peak heart rate, beats per minute

  During each experimental condition (at peak exercise during incremental ramp cycling test)

• Blood lactate concentration, mmol/L

  Capillary blood lactate was measured before the metabolically demanding exercise task, and then 1 minute, 3 minutes, 5 minutes, 30 minutes and 60 minutes post-exercise in both conditions.

• Jump height, cm

  Countermovement jump (CMJ) height was measured before (baseline), and then 1 minute, 30 minutes, and 60 minutes after the metabolically demanding exercise task in both conditions.

Study Arms (1)

male healthy volunteers aged 18-37 years

EXPERIMENTAL

Participants completed both conditions in a crossover design with washout. They consumed 10 g broccoli powder (\~320 μg glucoraphanin) or matched spinach placebo for 14 days (10 doses), final dose 3 h pre-test.

Dietary Supplement: Broccoli powder; Dietary Supplement: Placebo (no broccoli-derived bioactives)

Interventions

Broccoli powder DIETARY_SUPPLEMENT

The intervention consisted of a single 35-mL scoop of broccoli powder (BrocAffex; 99.5% broccoli powder and 0.5% mustard seed powder), corresponding to 10 g of supplement and delivering 320 μg of glucoraphanin per serving. The supplement was mixed with 300 mL of chocolate oat milk (13 participants) or 300 mL of orange juice (4 participants).

male healthy volunteers aged 18-37 years

Placebo (no broccoli-derived bioactives) DIETARY_SUPPLEMENT

1/2 x 35 ml scoop of dried spinach powder blended with 300 ml of chocolate oat milk (13 participants) or orange juice (4 participants).

male healthy volunteers aged 18-37 years

Eligibility Criteria

• Age: 18 Years - 37 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• males;
• age:18-37 years;
• no illnesses or injuries in the month preceding data collection and were engaged in organized physical activity no more than twice per week.

You may not qualify if:

• Non-compliance with the study protocol, illness, or injury during the study period.

Sponsors & Collaborators

• Lithuanian Sports University: lead

Study Sites (1)

Lithuanian Sports University

Kaunas, Lithuania

Location

Study Officials

• Sigitas Kamandulis, Dr.

  Lithuanian Sports University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 5, 2026
• First Posted: March 6, 2026
• Study Start: November 30, 2024
• Primary Completion: January 1, 2025
• Study Completion: January 10, 2025
• Last Updated: March 6, 2026

Record last verified: 2026-02

Data Sharing

• IPD Sharing: Will not share

Locations

LI (1)