Effects of 12-Week Balanced Diet on Body Composition & Metabolic Parameters in Twins

NCT07362550 | Recruiting

• 1 other identifier: UniversityUVAS1
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Twin study in Lahore will assess genetic vs. environmental effects on diet and metabolism. Over 12 weeks, monozygotic and dizygotic twins will follow a WHO-aligned balanced diet. Body composition, metabolic markers, and adherence will be measured. Monozygotic twins are expected to show greater similarity in response, clarifying personalized nutrition strategies in Pakistan.

Conditions

Healthy Adults

Keywords

Monozygotic; Dizygotic; Metabolic Parameters

Outcome Measures

Primary Outcomes (1)

• Change in Body Fat Percentage (%)

  Change in total body fat percentage measured using the InBody analyzer to assess the effect of a 12-week balanced diet intervention.

  Baseline and after 12-Week of Intervention.

Secondary Outcomes (3)

• Change in Fasting Plasma Glucose (mg/dL)

  Baseline and after 12 weeks

• Change in Lipid Profile (mg/dL)

  Baseline and after 12 weeks

• To compare within-pair similarity of Dietary Response

  Baseline and after 12-Weeks of intervention

Study Arms (2)

Habitual Diet

NO INTERVENTION

Control group will remain on habitual Diet.

Balanced Diet

EXPERIMENTAL

12-week culturally adapted WHO-aligned balanced diet emphasizing whole grains, lean proteins, healthy fats, fruits, and vegetables, individualized based on basal metabolic rate (BMR) and physical activity levels will be provided to interventional arm.

Other: Balanced Diet

Interventions

Balanced Diet OTHER

12-week culturally adapted WHO-aligned balanced diet emphasizing whole grains, lean proteins, healthy fats, fruits, and vegetables. Macronutrient composition: 45-65% carbohydrates, 20-35% fats, 10-20% protein. Individualized based on basal metabolic rate (BMR) and physical activity. Weekly counseling and monitoring through dietary recalls and photographic diaries. This intervention aims to assess the effects of a balanced diet on body composition and metabolic parameters in monozygotic and dizygotic twins.

Balanced Diet

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male or female twins aged 18-60 years.
• Confirmed zygosity (MZ or DZ) based on a validated twin zygosity questionnaire.
• Both twins willing to participate for the entire intervention period.
• Free from chronic diseases requiring specialized diets (e.g., type 1 diabetes, celiac disease).
• Not currently enrolled in another dietary or lifestyle intervention study.

You may not qualify if:

• Pregnant or lactating women.
• Use of lipid-lowering, glucose-lowering, or weight-loss medications in the past 3 months.
• Major gastrointestinal disorders or conditions affecting nutrient absorption.
• Unwillingness to follow individualized dietary counseling or complete study assessments.

Sponsors & Collaborators

• University of Veterinary and Animal Sciences, Lahore - Pakistan: lead

Study Sites (1)

University of Veterinary and Animal Sciences, Lhr.

Lahore, Punjab Province, 54000, Pakistan

RECRUITING

Study Officials

• Qaisar Raza, Ph.D

  UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES, LHR.

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Momina Azhar Mehmood, Postgraduate

CONTACT

+92-3227642452; mominaazhar1612@gmail.com

Qaisar Raza, Ph.D

CONTACT

+92-3002479044; qaisar.raza@uvas.edu.pk

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Model Details: Parallel, randomized, twin-pair controlled interventional study in which one twin from each monozygotic and dizygotic pair receives a 12-week WHO-aligned balanced diet while the co-twin continues habitual diet, with pre- and post-intervention assessments of body composition and metabolic parameters.

Study Record Dates

• First Submitted: December 17, 2025
• First Posted: January 23, 2026
• Study Start: January 17, 2026
• Primary Completion: April 19, 2026
• Study Completion: April 25, 2026
• Last Updated: January 23, 2026

Record last verified: 2025-12

Data Sharing

• IPD Sharing: Will not share

Locations

PA (1)