Multi-Modal Longevity Protocol Including Autologous Cell-Free Conditioned Media

NCT07322224 | Completed

• 1 other identifier: BB-ART3-2024-PILOT
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

This pilot study evaluated a multi-modal longevity protocol combining lifestyle optimisation, nutritional supplementation, and autologous cell-free conditioned media derived from peripheral blood-derived pro-regenerative cells (APRC-CM). Fourteen healthy adult participants completed a 17-week programme including baseline and post-intervention biomarker assessments. The primary objective was to characterise changes in biological age using DNA methylation-based epigenetic clocks, PhenoAge and other physiological and biochemical markers.

Conditions

Biological Aging; Healthy Aging; Longevity

Keywords

Epigenetic age; Phenotypic age; Autologous conditioned media; Longevity protocol; Supplements; Lifestyle

Outcome Measures

Primary Outcomes (1)

• Change in Epigenetic Biological Age

  Biological age calculated from DNA methylation profiles using validated epigenetic clock algorithms.

  Baseline and Week 17

Secondary Outcomes (1)

• Change in Phenotypic Age

  Baseline and Week 17

Other Outcomes (1)

• Incidence of Adverse Events

  From baseline to Week 17

Study Arms (1)

Longevity Protocol Arm

EXPERIMENTAL

Participants received a structured, multi-modal longevity protocol consisting of lifestyle optimization, nutritional supplementation, and autologous cell-free conditioned media (APRC-CM) prepared individually from peripheral blood-derived pro-regenerative cells. All participants received the same intervention schedule over a 17-week period.

Behavioral: Multi-Modal Longevity Protocol

Interventions

Multi-Modal Longevity Protocol BEHAVIORAL

The intervention consisted of a structured, multi-modal longevity program that included lifestyle optimization (nutrition, physical activity, and sleep routines), nutritional supplementation, and autologous cell-free conditioned media (APRC-CM). APRC-CM was prepared individually for each participant from peripheral blood-derived pro-regenerative cells and administered as part of a physiologic, minimal-risk exploratory protocol.

Longevity Protocol Arm

Eligibility Criteria

• Age: 45 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Age 45 years or older
• Generally good overall health as assessed by the study team
• Able and willing to provide written informed consent
• Willing to participate in all components of the study protocol, including lifestyle optimisation, nutritional supplementation, blood collection, and scheduled follow-up visits
• Able to comply with study procedures for the duration of the intervention and assessments

You may not qualify if:

• Presence of a major medical condition that, in the opinion of the investigators, could affect participation or safety
• Any condition requiring hospitalization or emergency medical intervention within the past 12 months
• Receipt of anti-aging or longevity treatments within the past six months (e.g., intravenous therapies, regenerative or cellular treatments, structured age-reversal programmes)
• Active infectious disease at enrolment
• Pregnancy or breastfeeding
• Use of systemic immunomodulatory, cytotoxic, or investigational treatments in the prior 3 months
• Any condition or circumstance that, in the judgement of the investigators, may interfere with study participation or data integrity

Sponsors & Collaborators

• BlueBird Age Reversal SA: lead
• xLongevity: collaborator
• Wellbeing International Foundation: collaborator

Study Sites (1)

Wellbeing International Foundation

London, W1G 0PG, United Kingdom

Location

Study Officials

• Esteban Ortega, MD

  Wellbeing International Foundation

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Model Details: Single-arm exploratory study in which all participants received the same multi-modal longevity protocol, including lifestyle optimisation, nutritional supplementation, and autologous cell-free conditioned media (APRC-CM). No comparator group was included.

Study Record Dates

• First Submitted: December 5, 2025
• First Posted: January 7, 2026
• Study Start: August 5, 2024
• Primary Completion: December 15, 2024
• Study Completion: March 20, 2025
• Last Updated: January 7, 2026

Record last verified: 2025-12

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)