Effect of Chronic Exercise on Human Thermogenic Adipose Tissue & Elucidation of Mechanisms

NCT07299604 | Completed

• 2 other identifiers: 2025-ExBatTRR333
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Physical exercise can stimulate heat production in our bodies. This is healthy because it burns sugar and fat. Heat production is influenced, among other things, by what is known as 'brown or beige adipose tissue', which is capable of producing heat in our bodies. However, everyone reacts differently to physical exercise. In addition, different types of exercise can influence heat production. That is why the investigators want to use this study to investigate why different people show different reactions in brown and beige adipose tissue. This is important for our metabolism and our health. The study will last approximately 6 weeks. The participants are healthy individuals who are participating in the study. The test subjects will be divided into two groups by drawing lots, so that there are equal numbers of men and women in each group. One group will complete a 6-week endurance training programme. The other group (control) will not do any additional training and will serve as a control group. However, the accompanying examinations will be the same for both groups. By comparing the two groups, the investigators will be able to see whether training has a better effect on heat production and thus health. As part of this study, the investigators want to investigate how 6 weeks of endurance training affects the function of brown and beige adipose tissue. To do this, the investigators will take some blood and adipose tissue samples before and after the training period.

Conditions

Endurance Training; No Training

Keywords

brown adipose tissue; activation; endurance training; oxygen consumption

Outcome Measures

Primary Outcomes (1)

• UCP-1 gene expression in cultured adipocytes treated with conditioned human sera

  The investigators collect human serum samples before/after training/control period and before/after cold exposure and exercise testing. Human adipose tissue cells will be treated with the conditioned serum samples of the training and control groups. The primary outcome measure is the change of mRNA expression of UCP-1 in adipose tissue cells by PCR.

  Serum samples will be collected at baseline and after six week of endurance training or six week of control period. Cell culture experiments will start when the last participant completed the study.

Secondary Outcomes (6)

• Energy expenditure in reaction to cold exposure

  Cold exposure will be done before and after the training or control period of six weeks.

• Physical performance analysis (oxygen consumption in ml/kg body weight)

  Physical performance analysis will be done before and after the training or control period of six weeks.

• Physical performance analysis (heart rate in bpm)

  Physical performance analysis will be done before and after the training or control period of six weeks.

• Physical performance analysis (blood lactate in mmol/L)

  Physical performance analysis will be done before and after the training or control period of six weeks.

• Physical performance analysis (power output in W)

  Physical performance analysis will be done before and after the training or control period of six weeks.

Other Outcomes (5)

• exomiRs screening by PCR/sequencing

  Serum samples will be collected at baseline and after six week of endurance training or six week of control period. Cell culture experiments will start when the last participant.

• Metabolomics screening (relative quantities)

  Plasma samples will be collected at baseline and after six week of endurance training or six week of control period.

• Proteomics screening (relative quantities)

  Plasma samples will be collected at baseline and after six week of endurance training or six week of control period.

Study Arms (2)

Endurance training

EXPERIMENTAL

The participants fulfill an individualized and supervised training. The 6 weelk training period consits of three training sessions per week.

Behavioral: Endurance Training

Control group

NO INTERVENTION

In parallel to the intervention group the control group only fulfill the inclusion measures and the follow-up measusres without any additional training. They will be advised to keep constant there normal physical activity, which has to be documented in dairy book.

Interventions

Endurance Training BEHAVIORAL

6 weeks of endurance training, 3time per week

Endurance training

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• BMI 18-25
• male/female
• less than 3 hours/week of regular training
• must be able to perform performance analysis and endurance training by cycling
• must be able to do six weeks of training

You may not qualify if:

• cardiovascular, pulmonary, neoplastic, orthopaedic, metabolic, or chronic diseases
• problems that preclude performance testing or endurance testing
• pregnancy (only female)
• regular medication
• smoking
• dependency relationship with the TUM Professorship of Exercise Biology

Sponsors & Collaborators

• Technical University of Munich: lead
• German Research Foundation: collaborator
• University Hospital, Bonn: collaborator
• Helmholtz Center Munich: collaborator

Study Sites (1)

University Munich, Professorship of Exercise Biolog

Munich, Bavaria, 80809, Germany

Location

Related Links

• Project website

MeSH Terms

Interventions

Endurance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Alexander Braunsperger, PhD candidate

  Technical University Munich, Professorship of Exercise Biology, Am Olympacampus 11, 80809 Munich

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: The investigators will recruit 24 participants, who will be randomised into two arms: 1) 6 weeks of individualised/supervised endurance training; 2) a control group without any additional training.

Study Record Dates

• First Submitted: November 14, 2025
• First Posted: December 23, 2025
• Study Start: January 1, 2025
• Primary Completion: June 30, 2025
• Study Completion: June 30, 2025
• Last Updated: December 23, 2025

Record last verified: 2025-12

Data Sharing

• IPD Sharing: Will not share

Locations

DE (1)