NCT07294963

Brief Summary

This study investigates whether a structured lifestyle program can help improve thinking skills and liver health in adults with Non-Alcoholic Fatty Liver Disease (NAFLD). We are enrolling 45 participants, aged 18-42, who will be randomly assigned to one of three groups for six months: one receiving general health advice, a second following a supervised Mediterranean diet plan, and a third combining the same diet with a regular walking program. The main goal is to see if these diet and exercise interventions can lead to better scores on memory and reasoning tests, reduce liver stiffness measured by a painless scan (FibroScan), and improve related blood markers of inflammation and hormone balance.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
45

participants targeted

Target at P25-P50 for not_applicable

Timeline
2mo left

Started Oct 2025

Shorter than P25 for not_applicable

Geographic Reach
1 country

2 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress85%
Oct 2025Jun 2026

Study Start

First participant enrolled

October 1, 2025

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

December 8, 2025

Completed
11 days until next milestone

First Posted

Study publicly available on registry

December 19, 2025

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 28, 2026

Completed
2 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 15, 2026

Expected
Last Updated

December 19, 2025

Status Verified

December 1, 2025

Enrollment Period

7 months

First QC Date

December 8, 2025

Last Update Submit

December 8, 2025

Conditions

Keywords

Hepatocerebral SyndromeMetabolic dysfunction-Associated SteatoHepatitisCognitive DeclineLiver FibrosisNon-Alcoholic Fatty Liver Disease

Outcome Measures

Primary Outcomes (4)

  • Composite of Heart Failure Hospitalization or Cardiovascular Death

    Time to first adjudicated event of either hospitalization for worsening heart failure or cardiovascular death. Events will be confirmed by a blinded Clinical Events Committee (CEC).

    From randomization up to study completion (maximum follow-up of 30 months).

  • Change in Kansas City Cardiomyopathy Questionnaire (KCCQ) Overall Summary Score

    The Kansas City Cardiomyopathy Questionnaire (KCCQ) is a 23-item, self-administered disease-specific questionnaire. The Overall Summary Score (OSS) aggregates the physical limitation, symptom frequency, quality of life, and social limitation domains. Scores range from 0 to 100, where higher scores indicate better health status. The outcome is the change in KCCQ-OSS from baseline.

    Baseline, Month 12.

  • Change in N-terminal pro-brain natriuretic peptide (NT-proBNP)

    The ratio of change in serum NT-proBNP level from baseline. NT-proBNP will be measured in pg/mL by a central laboratory using a standardized assay.

    Baseline, Month 6.

  • All-cause Mortality

    Time from randomization to death from any cause.

    From randomization up to study completion (maximum follow-up of 30 months).

Study Arms (3)

Mediterranean Diet Intervention

EXPERIMENTAL

Participants will follow a structured, culturally adapted Mediterranean diet plan for 6 months, designed and supervised by a qualified dietitian. The diet emphasizes whole grains, vegetables, fruits, legumes, nuts, olive oil, and lean proteins (detailed 7-day plan in Annexure 7). Support includes individualized counseling, provision of meal plans, weekly food diaries, 24-hour dietary recalls, and monthly check-ins. Outcome assessments occur at T0, T1 (6 months), and T2 (12 months).

Behavioral: Structured Mediterranean Diet

Control / General Advice

ACTIVE COMPARATOR

Participants will receive general verbal and written advice on healthy eating and physical activity, consistent with standard care. No structured diet plan or supervised exercise will be provided. They will undergo all outcome assessments (FibroScan, FIB-4, MoCA, biomarker blood draws) at Baseline (T0), 6 months (T1), and 12 months (T2).

Behavioral: Control / General Advice

Combined Diet and Exercise Intervention

EXPERIMENTAL

Participants will receive the identical Mediterranean diet intervention as Arm 2 plus a structured, progressive aerobic exercise program. The exercise consists of a 60-minute walking session, 5 days per week, for 6 months, progressing through conditioning, strength-building, and endurance phases (detailed in Annexure III). Compliance is monitored via exercise logs, fortnightly contact, and monthly check-ins. Outcome assessments occur at T0, T1 (6 months), and T2 (12 months).

Behavioral: Structured Mediterranean DietBehavioral: Control / General Advice

Interventions

A culturally adapted, structured dietary plan based on the Mediterranean diet principles, delivered through individual dietitian counseling, provision of weekly meal plans, and ongoing compliance support (food diaries, recalls, monthly check-ins).

Combined Diet and Exercise InterventionMediterranean Diet Intervention

This intervention consists of providing standardized, non-structured verbal and written information on the principles of a balanced diet and the benefits of regular physical activity, as per routine clinical practice. It serves as the active comparator representing minimal intervention.

Combined Diet and Exercise InterventionControl / General Advice

Eligibility Criteria

Age18 Years - 42 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Age 18 to 42 years.
  • Diagnosed with fatty liver (NAFLD) via imaging (e.g., ultrasound).
  • Liver fibrosis assessed and staged via FibroScan and the FIB-4 index.
  • Literate (to consent and complete cognitive assessments/questionnaires).

You may not qualify if:

  • History of alcohol intake \>20g/day.
  • Chronic viral hepatitis B or C.
  • Major neuropsychiatric illnesses.
  • Pregnancy or breastfeeding.
  • Chronic illnesses including Diabetes Mellitus and thyroid dysfunction.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Khyber Teaching Hospital

Peshawar, KPK, 25100, Pakistan

RECRUITING

Northwest General Hospital

Peshawar, KPK, 25100, Pakistan

RECRUITING

Related Publications (4)

  • Kjaergaard K, Mikkelsen ACD, Wernberg CW, Gronkjaer LL, Eriksen PL, Damholdt MF, Mookerjee RP, Vilstrup H, Lauridsen MM, Thomsen KL. Cognitive Dysfunction in Non-Alcoholic Fatty Liver Disease-Current Knowledge, Mechanisms and Perspectives. J Clin Med. 2021 Feb 9;10(4):673. doi: 10.3390/jcm10040673.

    PMID: 33572481BACKGROUND
  • Semmler G, Datz C, Reiberger T, Trauner M. Diet and exercise in NAFLD/NASH: Beyond the obvious. Liver Int. 2021 Oct;41(10):2249-2268. doi: 10.1111/liv.15024. Epub 2021 Aug 21.

    PMID: 34328248BACKGROUND
  • Whitehead AL, Julious SA, Cooper CL, Campbell MJ. Estimating the sample size for a pilot randomised trial to minimise the overall trial sample size for the external pilot and main trial for a continuous outcome variable. Stat Methods Med Res. 2016 Jun;25(3):1057-73. doi: 10.1177/0962280215588241. Epub 2015 Jun 19.

    PMID: 26092476BACKGROUND
  • Ishiba H, Sumida Y, Tanaka S, Yoneda M, Hyogo H, Ono M, Fujii H, Eguchi Y, Suzuki Y, Yoneda M, Takahashi H, Nakahara T, Seko Y, Mori K, Kanemasa K, Shimada K, Imai S, Imajo K, Kawaguchi T, Nakajima A, Chayama K, Saibara T, Shima T, Fujimoto K, Okanoue T, Itoh Y; Japan Study Group of Non-Alcoholic Fatty Liver Disease (JSG-NAFLD). The novel cutoff points for the FIB4 index categorized by age increase the diagnostic accuracy in NAFLD: a multi-center study. J Gastroenterol. 2018 Nov;53(11):1216-1224. doi: 10.1007/s00535-018-1474-y. Epub 2018 May 9.

    PMID: 29744597BACKGROUND

MeSH Terms

Conditions

Non-alcoholic Fatty Liver DiseaseNeurocognitive DisordersCognitive DysfunctionLiver Cirrhosis

Condition Hierarchy (Ancestors)

Fatty LiverLiver DiseasesDigestive System DiseasesMental DisordersCognition DisordersFibrosisPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • Dr Ayesha Qaiser, PhD Scholar

    Khyber Medical University Peshawar

    PRINCIPAL INVESTIGATOR
  • Dr Inayat Shah, PhD

    Institute of Basic Medical Sciences, Khyber Medical University

    PRINCIPAL INVESTIGATOR
  • Dr Arshad Hussain, PhD

    Northwest General Hospital, Peshawar

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Dr Ayesha Qaiser, MBBS, PhD Scholar

CONTACT

Dr Inayat Shah, PhD

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Masking Details
Outcome assessors conducting the Montreal Cognitive Assessment (MoCA) and laboratory technicians performing biomarker analyses (ELISA for T-Tau, cytokines, hormones) will be blinded to group assignment. Participants and the interventionists (dietitians, physiotherapists) cannot be blinded due to the behavioral nature of the lifestyle interventions.
Purpose
TREATMENT
Intervention Model
PARALLEL
Model Details: A three-arm parallel-group randomized controlled trial will be conducted. Participants will be assigned to the Mediterranean Diet intervention, Control (general advice 2), and combined Mediterranean Diet + Structured Exercise intervention.
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 8, 2025

First Posted

December 19, 2025

Study Start

October 1, 2025

Primary Completion

April 28, 2026

Study Completion (Estimated)

June 15, 2026

Last Updated

December 19, 2025

Record last verified: 2025-12

Data Sharing

IPD Sharing
Will share

De-identified individual participant data that underlie the results reported in the primary publication (including demographic, clinical, laboratory, and cognitive assessment data) will be made available to qualified researchers upon reasonable request. The data will be shared to achieve the aims in the approved proposal, subject to a data use agreement.

Shared Documents
STUDY PROTOCOL, SAP, ICF, CSR
Time Frame
Data will become available 9 months after the publication of the primary results and will be accessible for 5 years.
Access Criteria
Data access requests should be directed to the corresponding author (Dr. Ayesha Qaiser) and will be reviewed by the trial's steering committee. Requestors will need to provide a methodologically sound proposal and sign a data use/access agreement.

Locations