Use of PNF and Tissue Flossing to Treat Hamstring Tightness in Young Female Students

NCT07178132 | Completed

• 1 other identifier: 2250/HG
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to learn tissue flossing and proprioceptive neuromuscular facilitation effect in treating hamstring muscle tightness. The main questions it aims to answer are:

1. 1.which technique is more useful in giving instant relaxation of hamstring muscle
2. 2.can these technique increase muscle flexibility and knee motion
3. 3.be provided one technique either tissue flossing or PNF in first day
4. 4.physiotherapist will immediately measure the the change in flexibility or knee motion

Conditions

Hamstring Tightness; Muscle Tightness

Keywords

tightness; tissue flossing; proprioceptive neuromuscular facilitation; Hamstring tightness; flexibility

Outcome Measures

Primary Outcomes (4)

• Active Knee Extension Test

  With the participant supine and the hip flexed to 90°, the participant actively extends the knee. A goniometer was used to records the knee extension angle. Three trials are performed and the mean is used for analysis.

  From enrollment to the end of treatment at 1 week

• Passive Knee Extension Test

  With the participant supine and the hip flexed to 90°, the examiner passively extends the knee until the first onset of resistance. A goniometer is used to records the knee extension angle. Three trials are performed and the mean is used.

  From enrollment to the end of treatment at 1 week

• Straight Leg Raise

  With the participant supine, the examiner passively raises the extended leg until resistance . A goniometer at the hip measures maximum hip flexion angle (degrees). Three trials are performed and the mean is used.

  From enrollment to the end of treatment at 1 week

• Sit-and-Reach Test

  The participant sits with legs extended and reaches forward along a standard sit-and-reach box . After a warm-up, three trials are performed and the mean score (cm) is recorded.

  From enrollment to the end of treatment at 1 week

Study Arms (2)

Tissue Flossing Arm

EXPERIMENTAL

Application of tissue floss band to hamstring muscle at moderate tension for 2 minutes, followed by flexibility testing via AKET (Active Knee Extension Test), PKET (Passive Knee Extension Test), SLR (Straight Leg Raise), and Sit-and-Reach.

Other: tissue floss band

PNF Hold-Relax Arm

ACTIVE COMPARATOR

Standard PNF hold-relax stretching technique of hamstring, followed by flexibility testing via AKET (Active Knee Extension Test), PKET (Passive Knee Extension Test), SLR (Straight Leg Raise), and Sit-and-Reach.

Other: Proprioceptive Neuromuscular Facilitation

Interventions

tissue floss band OTHER

Floss band is wrapped in thigh from distal to proximal for 2 minutes. during this period patient is asked to actively perform movement for 2 minute, after that the band is removed and repeated once more. Immediately followed by flexibility measurements

Also known as: Floss band

Tissue Flossing Arm

Proprioceptive Neuromuscular Facilitation OTHER

Proprioceptive Neuromuscular Facilitation (PNF) Stretching using the hold-relax method: participants perform an isometric contraction of the hamstring at the end of its stretch range , followed by relaxation and passive stretch, then flexibility assessed similarly

PNF Hold-Relax Arm

Eligibility Criteria

• Age: 18 Years - 25 Years
• Gender Eligibility Details: Only female participants were eligible for inclusion in this study. The study focused on young women because sex-related differences in hamstring flexibility, joint range of motion, and neuromuscular properties have been reported in the literature. Restricting the sample to female students provided a more homogeneous group and reduced variability related to gender differences, thereby enhancing internal validity
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female subjects were included in the study
• Subjects with hamstring muscle tightness were included
• Subjects between the age group of 18-25 years were included

You may not qualify if:

• Male subjects
• Subject who didn't fulfill the age criteria
• Subject with hamstring tear or other knee ligament injury
• Any history of lower limb surgery dating back a year
• Subjects with Lower back pain or any neurological compromise in the lower limb
• Any acute injury like a sprain, strain, and open wound
• Subjects who had latex allergies
• Subjects with venous thrombotic disease, heart disease, respiratory illness, and dermatitis, in their lower legs were excluded

Sponsors & Collaborators

• Sakshi Negi: lead
• Guru Nanak Dev University: collaborator

Study Sites (1)

Guru Nanak Dev University

Amritsar, Punjab, India

Location

MeSH Terms

Interventions

Muscle Stretching Exercises

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Manpreet Kaur, MPT

  Guru Nanak Dev University

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: September 10, 2025
• First Posted: September 17, 2025
• Study Start: February 2, 2024
• Primary Completion: April 10, 2024
• Study Completion: April 15, 2024
• Last Updated: September 24, 2025

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will not share

Locations

IN (1)