Effects of One HIIT Session on Adiponectin Isoforms

NCT07146867 | Completed DMC

• 1 other identifier: AcuteHIIT
• Study Type: interventional
• Target: 21
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this observational quasi-experimental study is to evaluate whether a single session of high-intensity interval training (HIIT) can acutely modulate metabolic markers, particularly adiponectin isoforms, in insufficiently active young adults (men and women, 18-29 years, free of cardiometabolic disease). The main questions it aims to answer are: Does a single HIIT session increase circulating medium- and high-molecular-weight adiponectin? Does HIIT acutely alter lipid and glucose metabolism markers such as non-HDL cholesterol, insulin, and lactate? Participants completed the following tasks: Underwent baseline assessments (anthropometry, body composition, and resting cardiovascular parameters). Performed one HIIT session consisting of six 3-minute intervals at 80% heart rate reserve, each followed by 3 minutes of active recovery. Provided blood samples before and immediately after exercise to analyze adiponectin isoforms (LMW, MMW, HMW) via Western blot and to measure glucose, insulin, lipid profile, liver enzymes, and lactate. Summary of findings: A single HIIT session significantly increased medium-molecular-weight adiponectin (\~19%), decreased the HMW/MMW ratio, and elevated lactate and non-HDL cholesterol. No significant changes were observed in LMW or HMW adiponectin. These results suggest that MMW adiponectin is an early, sensitive marker of acute exercise response in sedentary young adults, though further research is needed to confirm long-term implications

Conditions

Inactivity, Physical

Keywords

adiponectin; high intensity interval training

Outcome Measures

Primary Outcomes (2)

• Adiponectin isoforms

  Before and immediately after the exercise session

• Adiponectin isoforms

  Before and immediately after the exercise session (30 minutes)

Study Arms (1)

High intensity interval training

EXPERIMENTAL

One session of high intensity interval training

Behavioral: High intensity interval training (exercise)

Interventions

High intensity interval training (exercise) BEHAVIORAL

One 30 minute session of high intensity interval training

High intensity interval training

Eligibility Criteria

• Age: 18 Years - 29 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age between 18 and 29 years
• Engage in less than 150 minutes of moderate-intensity or 75 minutes of vigorous-intensity physical activity per week)
• Having no positive responses on the PAR-Q \& YOU questionnaire

You may not qualify if:

• Diagnosis of cardiometabolic disease
• Use of anti-inflammatory treatment during the week prior to participation

Sponsors & Collaborators

• Universidad San Sebastián: lead

Study Sites (1)

Universidad San Sebastián

Valdivia, Los Ríos Region, 5090000, Chile

Location

Related Publications (1)

• Alvarado-Carrasco D, Gonzalez-Maraboli A, Reyes-Montalva M, Videla-Aguilar J, Bentes A, Penailillo L, Sandoval V, Martinez-Huenchullan S. Acute effects of high-intensity interval training on adiponectin isoforms in inactive young adults: a quasi-experimental study. BMC Sports Sci Med Rehabil. 2025 Dec 30;17(1):387. doi: 10.1186/s13102-025-01433-7.

  PMID: 41469733 DERIVED

MeSH Terms

Conditions

Sedentary Behavior

Interventions

High-Intensity Interval Training; Exercise

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Researcher

Study Record Dates

• First Submitted: August 21, 2025
• First Posted: August 28, 2025
• Study Start: March 1, 2023
• Primary Completion: December 1, 2024
• Study Completion: January 15, 2025
• Last Updated: May 5, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, ICF

Locations

CL (1)