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Assessment of Biochemical, Metabolic, Inflammatory, and Hematological Parameters Following Acute Exercise in Normal-Weight and Overweight/Obese Adolescent Girls.
Sponsor study identifier
interventional
35
1 country
1
Study Start
August 10, 2025
Actual (reported)Primary Completion
August 13, 2025
Actual (reported)Study Completion
August 13, 2025
Actual (reported)The goal of this interventional clinical trial is to compare the acute physiological and biochemical responses to maximal physical exercise (Spartacus test). in two groups of adolescent girls: in normal-weight and overweight/obese adolescent. The main question it aims to answer:
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
August 13, 2025
Actual (reported)Click on a node to explore related trials.
Some registry-reported names shown below are not yet linked in this graph.
Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
July 18, 2025
Actual (reported)August 5, 2025
Actual (reported)August 10, 2025
Actual (reported)August 13, 2025
Actual (reported)August 13, 2025
Actual (reported)Concentration of Blood Glucose (mg/dL)
Blood glucose levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
iron
Serum iron concentration will be measured using a colorimetric assay in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Total Cholesterol (mg/dL)
Total cholesterol will be assessed using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Body mass index (BMI) between the 5th and \<85th percentile for age.
BMI ≥ 85th percentile for age.
The Spartacus test was conducted on a 750 m (75 × 10 m) rectangular course marked with cones at regular intervals to indicate different speeds (from 7 to 18 km/h). Each participant began running at 7 km/h, with speed increasing by 1 km/h every three minutes.During the 3-minute intervals (for each speed stage), participants had 15 seconds to reach the corresponding marker, followed by 15 seconds of rest. Participants had to adjust their running pace to reach the target cone in synchrony with an audio signal. The test ended when a participant failed to reach the next cone within the given time. This test is validated for assessing maximal aerobic capacity (MAS) in obese adolescents. During the Spartacus test, maximal heart rate (HRmax) was recorded using a heart rate monitor (S810; Polar, Kempele, Finland).
Registry-reported names are shown in full. Names without a link have not yet been matched to a Syfrah profile.
High Institute of Sports and Physical Education of Kef
Boulifa, Kef Governorate, 7100, Tunisia
Wissal abassi, Dr
Research Unit "Sport Sciences, Health and Movement"(UR22JS01) High Institute of Sport and Physical Education of Kef, University of Jendouba, 7100 Kef, Tunisia.
35 participants (actual enrollment reported by the registry).
First Submitted
July 18, 2025
First Posted
August 5, 2025
Study Start
August 10, 2025
Actual (reported)Concentration of HDL Cholesterol (mg/dL)
HDL cholesterol will be analyzed using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Triglycerides (mg/dL)
Triglyceride levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Calculated LDL Cholesterol (mg/dL)
LDL cholesterol will be estimated using the Friedewald formula based on total cholesterol, HDL cholesterol, and triglycerides measured via enzymatic colorimetric assays in a certified clinical laboratory. LDL-C (mg/dL)=Cholesterol total-HDL Cholesterol-( Triglycerides /5)
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of C-Reactive Protein (CRP) (mg/L)
CRP levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Erythrocyte Sedimentation Rate (ESR) (mm/hr)
ESR will be determined using standard laboratory methods in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Aspartate Aminotransferase (AST) (U/L)
AST levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Alanine Aminotransferase (ALT) (U/L)
ALT levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Lactate Dehydrogenase (LDH) (U/L)
LDH levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Creatine Kinase (CK) (U/L)
CK levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Creatinine (mg/dL)
Creatinine levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Urea (mg/dL)
Urea levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Concentration of Serum Uric Acid (mg/dL)
Serum concentrations of uric acid will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Glomerular Filtration Rate
The glomerular Filtration Rate (GFR) has been computed using the Cockcroft formula, as follows: GFR (mL/min) = \[(140 - age) × weight ×0.85\]/( Serum Creatinine× 72).
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
White Blood Cell Count (WBC) (10³/µL)
WBC will be measured using standard hematology analyzers in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Red Blood Cell Count (RBC) (10³/µL)
RBC will be measured using standard hematology analyzers in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Hemoglobin Concentration (Hb) (g/dL)
Hb concentration will be measured using standard hematology analyzers in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Hematocrit (Hct) (%)
Hematocrit will be measured using standard hematology analyzers in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Platelet Count (10³/µL)
Platelet count will be measured using standard hematology analyzers in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Mean Corpuscular Hemoglobin Content (MCH) (pg)
Blood levels of mean corpuscular hemoglobin content will be measured from a 5 ml blood sample and analyzed using a multichannel automated hematology analyzer in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Mean Hemoglobin Concentration (MCHC) (g/dL)
Blood levels of mean hemoglobin concentration will be measured from a 5 ml blood sample and analyzed using a multichannel automated hematology analyzer in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Mean Corpuscular Volume (MCV) (fL)
Blood levels of mean corpuscular volume will be measured from a 5 ml blood sample and analyzed using a multichannel automated hematology analyzer in a certified clinical laboratory
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Plasma volume variations
Plasma volume variations (PVV) was calculated based on measured hematocrit (Ht) and hemoglobin (Hb) valuesaccording to the method developed by Costill and Fink (1974). %PVV=100 ×\[(HbA/HbB) ×(100 - HtB) / (100 - HtA)\] - 1, where 0 is the value measured before exercise and 1 is the value measured after exercise.
Plasma volume variations (PVV) was calculated Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Sodium Concentration (mmol/L)
Serum sodium levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Potassium Concentration (mmol/L)
Serum potassium levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Chloride Concentration (mmol/L)
Serum chloride levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Calcium Concentration (mmol/L)
Serum calcium levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Phosphorus Concentration (mmol/L)
Serum phosphorus levels will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Magnesium Concentration (mg/dL)
Serum magnesium concentration will be determined using a spectrophotometric colorimetric method in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Serum Iron Concentration (µg/dL)
Serum iron concentration will be measured using enzymatic colorimetric assays in a certified clinical laboratory.
At baseline, Immediately after Spartacus, and 30 minutes post-Spartacus.
Contact the study team to confirm eligibility.
Primary Completion
August 13, 2025
Actual (reported)Study Completion
August 13, 2025
Actual (reported)Last Updated
September 11, 2025
Record last verified: 2025-07
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Source: ClinicalTrials.gov.
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