Comparison of Skin Condition After Hyaluronic Acid Application on Dry vs. Moist Skin

NCT07078474 | Completed

• 1 other identifier: 2181-198-03-04-25-0064
• Study Type: interventional
• Target: 23
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to compare the effects of two different methods of applying hyaluronic acid (HA) on skin parameters in healthy individuals. HA is a widely used ingredient in skincare products due to its hydrating properties. However, it is unclear whether applying HA to dry or pre-moistened skin provides better results in terms of skin hydration and texture. In this non-invasive, interventional trial, 23 healthy volunteers aged 18 to 50 will be randomly assigned to two groups. One group will apply HA to dry skin, while the other will apply HA to moistened skin. Skin parameters will be measured before and after the intervention to assess hydration, transepidermal water loss, and overall skin condition. The aim is to determine the optimal method of HA application that maximizes its effectiveness in improving skin quality. Participants may benefit from improved skin hydration and increased awareness of the best application method for their skin type. No significant risks are expected from participation.

Conditions

Skin Hydration; Hyaluronic Acid

Outcome Measures

Primary Outcomes (1)

• Change in skin hydration level

  Skin hydration will be measured at four time points using a corneometer or comparable non-invasive skin analysis device. Measurements will be taken before the intervention (baseline) and at the end of each week to assess cumulative and comparative effects of hyaluronic acid application.

  Baseline, Week 1, Week 2, Week 3

Study Arms (2)

Dry Skin Application

EXPERIMENTAL

Participants in this group applied hyaluronic acid topically to clean, dry skin. The aim was to evaluate hydration and skin parameter changes following this method of application.

Other: Topical Hyaluronic Acid

Moist Skin Application

EXPERIMENTAL

Participants in this group applied hyaluronic acid topically to pre-moistened skin. The goal was to assess whether applying the product to damp skin enhances its hydrating effect.

Other: Topical Hyaluronic Acid

Interventions

Topical Hyaluronic Acid OTHER

Hyaluronic acid was applied topically to the facial skin of healthy participants once daily for a period of 3 weeks. Two application methods were used: on dry skin and on pre-moistened skin. Skin measurements were performed at baseline and after each week of intervention (Week 1, Week 2, and Week 3) to assess hydration and other skin parameters.

Dry Skin Application; Moist Skin Application

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy individuals aged 18 to 50 years
• Willingness to apply the product daily for 3 weeks
• Ability to attend all scheduled skin assessments (baseline + weekly)
• Provided written informed consent

You may not qualify if:

• Known allergy or sensitivity to hyaluronic acid or any component of the product
• Active skin diseases or dermatological conditions in the facial area
• Use of systemic or topical corticosteroids or retinoids within 4 weeks prior to study
• Pregnancy or breastfeeding
• Participation in another clinical trial within the past 30 days

Sponsors & Collaborators

• University of Split, School of Medicine: lead

Study Sites (1)

University of Split School of Medicine

Split, Split-Dalmatia County, 21000, Croatia

Location

MeSH Terms

Interventions

Hyaluronic Acid

Intervention Hierarchy (Ancestors)

Glycosaminoglycans; Polysaccharides; Carbohydrates

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: July 11, 2025
• First Posted: July 22, 2025
• Study Start: June 18, 2025
• Primary Completion: July 9, 2025
• Study Completion: July 9, 2025
• Last Updated: July 22, 2025

Record last verified: 2025-06

Data Sharing

• IPD Sharing: Will not share

Locations

CR (1)