NCT07069075|Completed
Gene Polymorphisms, Skin Barrier, and Inflammation in Acne
The Relationship Between MMP-2 and TIMP-2 Gene Polymorphisms and Skin Barrier Function, Inflammatory Cytokine Levels in Acne Patients
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The Relationship Between MMP-2 and TIMP-2 Gene Polymorphisms and Skin Barrier Function, Inflammatory Cytokine Levels in Acne Patients
Sponsor study identifier
observational
300
1 country
1
Study Start
August 1, 2018
Actual (reported)Primary Completion
September 30, 2021
Actual (reported)Study Completion
September 30, 2021
Actual (reported)This study investigated the relationship between Matrix Metalloproteinase-2 (MMP-2) and Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) gene polymorphisms and acne pathogenesis. The study aimed to determine whether specific genotypes (MMP-2-CC and TIMP-2-CC) are associated with impaired skin barrier function (measured by transepidermal water loss and skin hydration) and elevated levels of inflammatory cytokines (IL-1β, TNF-α) in acne patients compared to healthy controls.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
September 30, 2021
Actual (reported)Click on a node to explore related trials.
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Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
August 1, 2018
Actual (reported)September 30, 2021
Actual (reported)September 30, 2021
Actual (reported)July 5, 2025
Actual (reported)July 16, 2025
Actual (reported)Genotype Frequencies of MMP-2 and TIMP-2
Comparison of the genotype distribution (CC vs. CT/TT for MMP-2; CC vs. GC/GG for TIMP-2) between acne patients and healthy controls to assess association with acne susceptibility. \[Time Frame: Data collected at a single study visit\] Skin Barrier Function Assessment: Measurement of Transepidermal Water Loss (TEWL) and skin hydration levels in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Skin Barrier Function Assessment
Measurement of Transepidermal Water Loss (TEWL) and skin hydration levels in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Serum Interleukin-1β (IL-1β) Level
Measurement of serum levels of IL-1β in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Serum Tumor Necrosis Factor-α (TNF-α) Level
Measurement of serum levels of TNF-α in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Correlation Analysis
Analysis was performed after the completion of data collection in September 2021.
Individuals aged 18 years or older who were diagnosed with acne vulgaris by a dermatologist. Participants provided blood samples for genotyping and cytokine analysis, and underwent non-invasive skin function tests.
Healthy volunteers aged 18 years or older without any systemic or skin diseases, recruited during the same period. Participants provided blood samples for genotyping.
Participants were genotyped for the MMP-2 rs243865 polymorphism. Genomic DNA was extracted from peripheral blood, and the specific genotype (CC, CT, or TT) was determined using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. This allowed for the stratification of participants based on their genetic variation at this locus for subsequent association analysis.
Patients with acne vulgaris and healthy volunteers recruited from the dermatology department of Shijiazhuang TCM Hospital.
Shijiazhuang TCM Hospital
Shijiazhuang, Hebei, 050000, China
Huang S, Fang L, Zhang M, Sun S, Zhi H, Zhu Y, Zhang H. The relationship between MMP-2 and TIMP-2 gene polymorphisms and skin barrier function, inflammatory cytokine levels in acne patients. Expert Rev Clin Immunol. 2026 Feb;22(2):233-241. doi: 10.1080/1744666X.2026.2636646. Epub 2026 Mar 4.
Venous blood samples were collected from all participants. Genomic DNA was extracted from whole blood for genotyping of MMP-2 rs243865 and TIMP-2 rs8179090. Serum was separated for measuring inflammatory cytokine levels (IL-1β, TNF-α) via ELISA.
300 participants (actual enrollment reported by the registry).
First Submitted
July 5, 2025
First Posted
July 16, 2025
Study Start
August 1, 2018
Actual (reported)Participants were genotyped for the TIMP-2 rs8179090 polymorphism. Genomic DNA was extracted from peripheral blood, and the specific genotype (CC, GC, or GG) was determined using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. This allowed for the stratification of participants based on their genetic variation at this locus for subsequent association analysis.
Registry-reported names are shown in full. Names without a link have not yet been matched to a Syfrah profile.
Contact the study team to confirm eligibility.
Primary Completion
September 30, 2021
Actual (reported)Study Completion
September 30, 2021
Actual (reported)Last Updated
July 16, 2025
Record last verified: 2025-07
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Source: ClinicalTrials.gov.
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